Персона: Сарапульцева, Елена Игоревна
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Елена Игоревна
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- ПубликацияТолько метаданныеRadiation-induced transgenerational effects in animals(2022) Dubrova, Y. E.; Sarapultseva, E. I.; Сарапульцева, Елена Игоревна© Copyright © 2020 Taylor and Francis Group LLC.According to the results of recent studies, parental exposure to ionizing radiation not only leads to mutation induction in the germline of irradiated animals but also affects their non-exposed offspring. These radiation-induced transgenerational effects belong to an epigenetic phenomenon that could not be defined as a transmission of altered phenotypes from the irradiated parents to their non-exposed offspring. In this review, we present the results of laboratory studies aimed to evaluate the transgenerational effects of parental irradiation on a number of traits in the offspring of exposed parents. The results of animal studies showing compromised viability, fertility and genome stability among the non-exposed offspring of irradiated parents are presented and discussed. So far, the epigenetic phenomenon of radiation-induced transgenerational effects has been established in laboratory studies. Future work should address the important issue of manifestation of radiation-induced transgenerational effects in populations inhabiting radioactive-contaminated areas, as well as the mechanisms of transgenerational effects.
- ПубликацияТолько метаданныеAnalytical Study of Patterns of the Survival Curves of Experimental Objects in Model Experiments on Daphnia magna(2021) Michalski, A. I.; Savina, N. B.; Bychkovskaya, I. B.; Sarapultseva, E. I.; Сарапульцева, Елена Игоревна© 2021, Pleiades Publishing, Ltd.Abstract: The phenomenon of multiphasic survival curves found in drosophila is confirmed in experiments on daphnids. Mathematical modeling showed that the multiphasic nature of daphnids survival curves reflected abrupt changes in the death rate of objects during phase transition. The phase of a sharp increase in mortality observed at an early age in intact drosophila occurs in daphnids only as reaction to a negative impact. The induction of reactive changes by weak external signals resulted in decreased survival for young daphnids. A logical conclusion was made about the reaction readiness for each experimental individual. It is proven that the predisposition to reaction in daphnia persists for an indefinitely large number of generations. It is postulated that this reaction has an epigenetic nature. Earlier experimental materials suggest that a reaction of this type is widespread in natural surroundings.
- ПубликацияОткрытый доступРАДИОСЕНСИБИЛИЗИРУЮЩИЕ СВОЙСТВА НАНОЧАСТИЦ ЗОЛОТА ПРИ ОБЛУЧЕНИИ ПРОТОНАМИ В ОПЫТАХ IN VIVO(НИЯУ МИФИ, 2024) Дегтярева, Д. Т.; Ускалова, Д. В.; Сабуров, В. О.; Кузьмичева, О. В.; Шахов, П. В.; Сарапульцева, Е. И.; Сарапульцева, Елена Игоревна; Шахов, Павел ВладимировичРадиосенсибилизаторы способны повышать чувствительногсть раковых клеток к облучению. Проведен анализ механизмов соче- танного действия наночастиц золота (AuНЧ) и облучения протонами на модели беспозвоночных животных Daphnia magna в опытах in vivo.
- ПубликацияОткрытый доступEnhancement of the Cytotoxic Effect of Proton Irradiation by Gold Nanoparticles(2024) Petrosova, D. T.; Uskalova, D. V.; Kuzmicheva, O. V.; Sarapultseva, E. I.; Сарапульцева, Елена ИгоревнаPurpose: To evaluate the bioefficiency and biosafety of proton irradiation in combination with gold nanoparticles (AuNPs) on a model of a higher invertebrate animal Daphnia magna from the suborder crustaceans in in vivo experiments. Materials and methods: The synthesis of AuNPs was carried out by a single-stage method of femtosecond laser ablation. A laboratory culture of Daphnia magna (D. magna) was used. Animals were cultured in a climatostat (model P2). Biological parameters (viability, fertility and cytotoxicity) were evaluated in two consecutive generations (F0) and (F1). Only animals of the parental generation (F0) were exposed to acute irradiation with a scanning proton beam (energy 150 MeV) on the Prometheus proton complex. Viability and fertility D. magna were evaluated in a 21-day experiment on a daily basis. A total of 10 to 60 individuals were analyzed in control and experimental groups. Cytotoxicity was analyzed by an MTT test modified for studies of the effect on invertebrates in in vivo experiments. From 11 to 97 samples were analyzed for cytotoxicity. There were 20 animals in each sample. The results are analyzed by methods of mathematical statistics adjusted for multiple comparisons. Results: Irradiation at doses of 10 and 30 Gy caused a decrease in animal viability, which was increased in 1.35 times. Reproductive dysfunction was found in both irradiated and first-generation animals. The use of AuNPs did not cause oxidative stress in D. magna, but increased the cytotoxic effect of proton irradiation. AuNPs contributed to the cytotoxic effect. Conclusions: Since the results obtained are consistent with the data published in the cited articles on vertebrates, it is possible to assume a universal mechanism of cytotoxic effect of proton irradiation in combination with AuNPs on both invertebrates and vertebrates, including humans, and the possibility of using AuNPs as radiosensitizers to enhance the effect of irradiation in binary proton therapy technologies.
- ПубликацияТолько метаданныеBiological effects of low-intensity radiofrequency fields and risk assessment for biota(2020) Uskalova, D. V.; Sarapultseva, E. I.; Ustenko, K. V.; Сарапульцева, Елена Игоревна© 2020, Institut meditsiny truda RAMN. All rights reserved.Despite the fact that there are still conflicting opinions about the damage caused by modern wireless communication technologies, most scientists report on the negative biological effects of low-intensity radio frequency electromagnetic radiation at different levels of the organization of live nature. There is no doubt that there is a need not only for a sanitary and hygienic assessment of man-made electromagnetic effects on humans, but also for an environmental assessment for biota. The purpose of the study was to assess the potential environmental risk of electromagnetic impact in the centimeter range on natural ecosystems. The initial data were the authors' own results in the field of radiobiology of non-ionizing radiation, as well as published of other researchers. The article analyzes the biological effects of radio frequency electromagnetic fields detected in organisms of different systematic groups and levels of organization. The data on the non-thermal biological effects of electromagnetic fields indicate a high sensitivity of different species to this factor. The analyzed research results emphasize the need to take into account the features of non-thermal effects of electromagnetic radiation on biota, since these radiations can have a negative impact on different hierarchical levels in natural ecosystems.
- ПубликацияТолько метаданныеBiophysical Methods for Testing Nanomaterials for Biomedical and Ecotoxicological Purposes Using Daphnia magna as a Model Organism(2024) Savina, N. B.; Uskalova, D. V.; Petrosova, D. T.; Sarapultseva, E. I.; Сарапульцева, Елена Игоревна
- ПубликацияТолько метаданныеTransgenerational changes in Daphnia magna under radio frequency radiation in the juvenile and puberty period(2023) Tikhonov, V. N.; Ivanov, I. A.; Tikhonov, A. V.; Sarapultseva, E. I.; Uskalova, D. V.; Ustenko, K. V.; Сарапульцева, Елена Игоревна© Copyright © 2022 Taylor and Francis Group LLC.Purpose: To analyze the results of direct and transgenerational effects of radio frequency electromagnetic fields (RF-EMF) on the model organism of crustaceans Daphnia magna. Materials and methods: D. magna were chronically exposed at 900 GHz EMF with an energy flux density (EFD) of about 1 mW/cm2 in the juvenile and pubertal periods of their ontogenesis. The cytotoxicity of exposure as well as survival, fertility and teratogenic effect of directly exposed daphnids and their progeny across three generations were analyzed. Results and conclusions: The results of our study show that exposure of RF-EMF at juvenile period can significantly affect the fertility and size of irradiated daphnids and their offspring of the first generation. The decrease in fertility may be associated with a cytotoxic effect on the cells of irradiated animals. The reduction in the size of the terminal spine and the body of individuals is an indicator of the negative impact of radiation on the protective strategy of the crustacean population. The reproductive process is restored by the second generation. The results of our study provide further insights into the possible mechanisms underlying the in vivo effects of RF-EMF.
- ПубликацияТолько метаданныеAn analytical study of the patterns of the survival curves of experimental objects in model experiments on Daphnia magna(2020) Michalski, A. I.; Savina, N. B.; Bychkovskaya, I. B.; Sarapultseva, E. I.; Сарапульцева, Елена ИгоревнаThe phenomenon of multiphase survival curves found in drosophila is confirmed in experiments on daphnia. Mathematical modeling showed that the multiphase nature of the daphnia survival curves reflects abrupt changes in the intensity of death of crustaceans during the transition from phase to phase. In intact daphnia there is no phase of a sharp increase in mortality observed at an early age in drosophila. It arises as a reaction to weak negative influences, which leads to a sharp decrease in the survival of young individuals. A logical conclusion was made about the readiness for reaction of each experimental individual. The effect was observed in a series of experiments posed in different years. It is proved that in daphnia, the predisposition to reaction persists for an indefinitely large number of generations. It is postulated that this reaction has an epigenetic nature. Previous experimental materials suggest that a reaction of this type is widespread in natural surroundings. Феномен многофазовости кривых дожития, найденный у дрозофилы, подтвержден в экспериментах на дафниях. Математическое моделирование показало, что многофазовость кривых дожития дафний отражает скачкообразные изменения интенсивности гибели объектов при переходе от фазы к фазе. У дафний фаза резкого повышения смертности, наблюдаемая в раннем возрасте у интактных дрозофил, появляется только как реакция на негативное воздействие. Индукция реактивных изменений слабыми внешними сигналами приводит к снижению выживаемости молодых дафний. Сделано логическое заключение о готовности к реакции каждой из экспериментальных особей. Доказано, что у дафний предрасположенность к реакции сохраняется в неопределенно большом ряду поколений. Постулируется, что эффект обусловлен эпигенетическими механизмами. Рассматриваются предыдущие экспериментальные материалы, позволяющие предположить, что реакция данного типа широко распространена в природе.
- ПубликацияОткрытый доступThe effects of electromagnetic field and γ-radiation on cell proliferation and regeneration in planarian Schmidtea mediterranea(2019) Matchuk, O. N.; Sarapultseva, E. I.; Uskalova, D.; Markina, H.; Savina, N.; Zamulaeva, I. A.; Сарапульцева, Елена Игоревна© Published under licence by IOP Publishing Ltd. We have shown that γ-irradiation at dose of 10 Gy and low-intensity electromagnetic fields (EMF, 900 MHz, 100 μW/cm 2 , 180 min) significantly compromised cell proliferation of planarian S. mediterranea regenerating after decapitation. Using flow cytometry we have shown that 24 hours after exposure to γ-rays and EMF the index of proliferation established as the total amount of cells at S and G2/M phases was 2.8 and 1.8 lower than that in controls respectively. Our data therefore provide the first experimental evidence for the effects of medium-dose γ-irradiation on regeneration in planaria which is attributed to radiation-induced compromised proliferation activity.
- ПубликацияТолько метаданныеReview of radiation technologies for the treatment of covid-19 coronavirus infection(2021) Gameeva, E. V.; Sarapultseva, E. I.; Garmash, A. A.; Gromushkina, E. V.; Maksarova, D. D.; Сарапульцева, Елена Игоревна; Гармаш, Александр Александрович; Громушкина, Елена Вячеславовна; Максарова, Дарима Дамбаевна© 2021 State Research Center, Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. All rights reserved.Due to the long-term lack of effective pharmacological concepts, the situation with the spread of a new coronavirus infection in 2019 (COVID-19) has aroused interest in considering the possible use of radiation technologies, including historical reports on the treatment of patients with pneumonia using low-dose radiation therapy. A brief review of articles on clinical trials of radiation technologies in the fight against COVID-19 is conducted. The authors of most of the analyzed articles, as well as the authors of this review, conclude that the available scientific data do not justify clinical trials of low-dose radiation therapy for the treatment of COVID-19 pneumonia due to the unclear benefits and risks of mortality from radiation-induced diseases, including radiogenic cancer and diseases of the circulatory system.