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Савельева, Татьяна Александровна

Научные группы

Научная группа

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Савельева

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Татьяна Александровна

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Теперь показываю 1 - 10 из 30

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Intraoperative control of hemoglobin oxygen saturation in the intestinal wall during anastomosis surgery
(2021) Kustov, D. M.; Kharnas, S. S.; Levkin, V. V.; Gorbunov, A. S.; Savelieva, T. A.; Mironov, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.During surgery for colon cancer, monitoring of the oxygen saturation of hemoglobin in the tissues under study makes it possible to assess the degree of blood supply to the anastomosis areas of the colon. Adequate blood supply in this area is decisive in terms of the consistency of the anastomosis and can significantly reduce the risk leakage of anastomosis. In this work, we propose a new approach to assessing the hemoglobin oxygen saturation based on measuring both the diffuse reflectance and transmittance spectra of the colon wall tissues. The proposed method is based on the use of two fiber-optic tools for irradiation from both sides—the intestinal lumen and the outside of the intestinal wall. The spectra are recorded from the external side. To determine the degree of hemoglobin saturation, two algorithms, both based on the Taylor series expansion of the coefficient of light attenuation by tissues, are proposed. The results of a clinical study of the proposed approach on volunteers were obtained, allowing to draw a conclusion about the applicability of the approach in a clinical setting.
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Fast Spectroscopic Technique of Optical Biopsy of Intracranial Tumors
(2024) Romanishkin, I. D.; Ospanov A.; Savelieva, T. A.; Shugai, S. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
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Method for rapid intraoperative analysis of the optical properties of multilayered walls of hollow organs
(2024) Savelieva, T. A.; Krivetskaya, A. A.; Levkin, V. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Кривецкая, Анна Александровна; Лощенов, Виктор Борисович
Открытый доступ
Study of possibility of cell recognition in brain tumors
(2020) Maklygina, Y. S.; Skobeltsin, A. S.; Savelieva, T. A.; Pavlova, G. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
The brain has an exceptionally high requirement for energy metabolism, with glucose serving as the exclusive energy source. Cancers, including glioblastoma, have a high glucose uptake and rely on aerobic glycolysis for energy metabolism. The alternation of high-efficiency oxidative phosphorylation to a low-efficiency aerobic glycolysis pathway (Warburg effect) provides macromolecules for biosynthesis and proliferation. Current research indicates that the specific metabolism in the tumor tissue and normal brain tissue in the glioma allows the use of 5-aminolevulinic acid (5 ALA)-induced protoporphyrin IX (PpIX) and methylene blue (MB) to monitor and correct the development of the tumor. The focus is on the detection of the differences between tumor cells and tumor-associated macrophages/microglia using spectroscopic and microscopic methods, based on the fluorescent signals and the difference in the drug accumulation of photosensitizers (PSs). Since 5 ALA has long been used effectively in the clinic for fluorescent surgical navigation, it was employed as an agent to identify the localization of tumor tissue and study its composition, particularly tumor and immune cells (macrophages), which have also been shown to actively accumulate PpIX. However, since PpIX is photodynamically active, it can be considered effective as the main target of tumor tissue for further successful photodynamic therapy. MB was employed to visualize resident microglia, which is important for their activation/deactivation to prevent the reprogramming of the immune cells by the tumor. Thus, using two drugs, it is possible to prevent crosstalk between tumor cells and the immune cells of different geneses.
Только метаданные
Evaluating the dynamics of brain tissue oxygenation using near-infrared spectroscopy on various experimental models
(2019) Kustov, D. M.; Sharova, A. S.; Makarov, V. I.; Borodkin, A. V.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2019 Astro Ltd.In this paper we consider a method for researching the dynamics of blood flow in the cerebral cortex, on an optical phantom that reproduces the parameters of real human and mice brain structures, through the use of near and infrared ranges of laser radiation. For the investigation of real tissue we chose a laboratory mouse brain in vivo. An algorithm for non-invasive diagnostics of the degree of oxygenation was identified and optimal parameters of installation components were selected for taking information about hemodynamic indicators. Output was verified by the reference method for assessing oxygenation by degree of absorption of hemoglobin in the visible range, which indicates that data have a high correlation with classical methods. With further development, this algorithm can be used in various areas of research and diagnostics.
Только метаданные
Combined spectroscopic and video fluorescent instrument for intraoperative navigation when removing a glial tumor
(2020) Loshchenov, M. V.; Borodkin, A. V.; Linkov, K. G.; Kosyrkova, A. V.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2020 SPIE.The new approach to intraoperative navigation during glial brain tumors removal is presented. A combined method is proposed for simultaneous spectroscopic and video fluorescence analysis of the state of tissues in the destruction zone using the applied part performed in the form of a neurosurgical aspirator cannula. In the walls of the applied part there are tubular channels into which lighting and receiving optical fibers are integrated. At the end of the cannula, the channels for optical fibers are arranged so as to perform spectroscopic analysis in contact with the surface of the biological tissue, as well as video fluorescence analysis at the working distance to the surface of the tissue. The joint use of fiber-optic systems for recording the video stream and spectral dependences allows real-time assessment of the degree of pathological tissue changes in the field of view of the video system, which are also located in the aspiration zone, with the simultaneous quantification of diagnostically significant spectroscopic criteria. System testing was carried out on samples of human intracranial tumors obtained during neurosurgical operations. During the removal of a tumor from different sites (tumor center, perifocal area), the degree of in vivo fluorescence signal from the tumor site was determined intraoperatively using a Zeiss Opmi Pentero intraoperative microscope in Blue 400 mode. From the selected area of the tumor, biopsy material was taken (presumably homogeneous in its properties) with subsequent measurement of spectra and combined images using the developed device. A high correlation was shown between the level of the fluorescence signal recorded spectroscopically and the brightness of the fluorescence image in the endoscopic channel of the device. The level of the fluorescent signal showed a high correlation with the degree of malignancy of tissues according to the results of pathomorphological examination.
Только метаданные
The optical estimation of glioma cell composition using fluorescence lifetime imaging
(2020) Maklygina, Y. S.; Romanishkin, I. D.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
Gliomas are one of the most common brain tumors occurring in children and adults. Gliomas are primary, diffusely infiltrating brain tumors. There are few effective therapies for these type of cancer, and patients with malignant glioma fare poorly, even after aggressive surgery, chemo- and radiotherapy.
Открытый доступ
Attenuation correction technique for fluorescence analysis of biological tissues with significantly different optical properties
(2020) Savelieva, T. A.; Kuryanova, M. N.; Akhlyustina, E. V.; Linkov, K. G.; Meerovich, G. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Ахлюстина, Екатерина Витальевна; Меерович, Геннадий Александрович; Лощенов, Виктор Борисович
During intraoperative fluorescence navigation to remove various neoplasms and during pharmacokinetic studies of photosensitizers in laboratory animals, in many cases, the ratio of photosensitizer accumulation in the tumor and normal tissue can reach > 10-fold, which inevitably changes their optical properties. At the same time, the tumor formation process causes various metabolic and structural changes at cellular and tissue levels, which lead to changes in optical properties. A hardware-software complex for the spectral-fluorescence studies of the content of fluorochromes in biological tissues with significantly different optical properties was developed, and it was tested on optical phantoms with various concentrations of photosensitizers, absorbers, and scatterers. To correct the influence of optical properties on the photosensitizer concentration analysis by fluorescence spectroscopy, we propose the spectrum-processing algorithm, which combines empirical and theory-based approaches.
Только метаданные
Raman spectroscopy for the development of a method for glial brain tumors diagnostics
(2020) Bikmukhametova, L. R.; Romanishkin, I. D.; Savelieva, T. A.; Orlov, A. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
Reducing the frequency of relapses after surgical removal of glial tumors is a non-trivial task due to their infiltrative growth. The main way to solve this problem is fluorescent intraoperative navigation. However, in the absence of accumulation of a fluorescent marker in the tumor tissues, other diagnostic parameters are required to find the boundaries of such tumors. Raman spectroscopy has the advantages of optical spectroscopy such as speed and non-invasiveness. With an exhaustive database of reference spectra of those components that can be expressed in glial tumors, Raman scattering spectroscopy allows multivariate diagnosis of such tumors and their intraoperative demarcation.
Только метаданные
Spontaneous raman spectroscopy for intracranial tumors diagnostics ex vivo
(2020) Romanishkin, I. D.; Bikmukhametova, L. R.; Goryaynov, S. A.; Kosyrkova, A. V.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2020 Russian Photodynamic Association. All rights reserved.Neurosurgery of intracranial tumors, especially of glial origin, is a non-trivial task due to their infiltrative growth. In recent years, optical methods of intraoperative navigation have been actively used in neurosurgery. However, one of the most widely used approaches based on the selective accumulation of fluorescent contrast medium (5-ALA-induced protoporphyrin IX) by the tumor cannot be applied to a significant number of tumors due to its low accumulation. On the contrary, Raman spectroscopy, which allows analyzing the molecular composition of tissues while preserving all the advantages of the method of fluorescence spectroscopy, does not require the use of an exogenous dye and may become a method of choice when composing a system for intraoperative navigation or optical biopsy. This work presents the first results of using the principal component method to classify Raman spectra of human glioblastoma with intermediate processing of spectra to minimize possible errors from the fluorescence of both endogenous fluorophores and photosensitizers used in fluorescence navigation. As a result, differences were found in the principal component space, corresponding to tissue samples with microcystic components, extensive areas of necrosis, and foci of fresh hemorrhages. It is shown that this approach can serve as the basis for constructing a system for automatic intraoperative tissue classification based on the analysis of Raman spectra.