Publication:
Detection of Influenza Virus Using a SOI-Nanoribbon Chip, Based on an N-Type Field-Effect Transistor

Файлы
W3157023377.pdf(1.64 MB)
Дата
2021
Авторы
Malsagova, K. A.
Pleshakova, T. O.
Kozlov, A. F.
Galiullin, R. A.
Nikitaev, V. G.
Pronichev, A. N.
Journal Title
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Научные группы
Организационные подразделения
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Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
Выпуск журнала
Аннотация
The detection of influenza A virions with a nanoribbon detector (NR detector) has been demonstrated. Chips for the detector have been fabricated based on silicon-on-insulator nanoribbon structures (SOI nanoribbon chip), using a complementary metal-oxide-semiconductor (CMOS)-compatible technology-by means of gas-phase etching and standard optical photolithography. The surface of the SOI nanoribbon chip contains a matrix of 10 nanoribbon (NR) sensor elements. SOI nanoribbon chips of n-type conductance have been used for this study. For biospecific detection of target particles, antibodies against influenza virus have been covalently immobilized onto NRs. Influenza A virus detection was performed by real-time registration of the source-drain current through the NRs. The detection of the target viral particles was carried out in buffer solutions at the target particles concentration within the range from 10(7) to 10(3) viral particles per milliliter (VP/mL). The lowest detectable concentration of the target viral particles was 6 x 10(-16) M (corresponding to 10(4) VP/mL). The use of solutions containing similar to 10(9) to 10(10) VP/mL resulted in saturation of the sensor surface with the target virions. In the saturation mode, detection was impossible.
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Цитирование
Detection of Influenza Virus Using a SOI-Nanoribbon Chip, Based on an N-Type Field-Effect Transistor / Malsagova, KA [et al.] // Biosensors. - 2021. - 11. - № 4. - 10.3390/bios11040119
URI
https://www.doi.org/10.3390/bios11040119
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85105134700&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000642807600001
 https://openrepository.mephi.ru/handle/123456789/24063
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