Publication:
Optimization of upconversion luminescence excitation mode for deeper in vivo bioimaging without contrast loss or overheating

Файлы
W3007667324.pdf(984.91 KB)
Дата
2020
Авторы
Pominova, D. V.
Romanishkin, I. D.
Proydakova, V. Y.
Grachev, P. V.
Ryabova, A. V.
Loschenov, V. B.
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Научные группы
Организационные подразделения
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Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Аннотация
Upconversion nanoparticles have attracted considerable attention as luminescent markers for bioimaging and sensing due to their capability to convert near-infrared (NIR) excitation into visible or NIR luminescence. However, the wavelength of about 970 nm is commonly used for the upconversion luminescence excitation, where the strong absorption of water is observed, which can lead to laser-induced overheating effects. One of the strategies for avoiding such laser-induced heating involves shifting the excitation into shorter wavelengths region. However, the influence of wavelength change on luminescent images quality has not been investigated yet. In this work, we compare wavelengths of 920, 940 and 970 nm for upconversion luminescence excitation in the thickness of biological tissues in terms of detected signal intensity and obtained image quality (contrast and signal-to-background ratio). Studies on biological tissue phantoms with various scattering and absorbing properties were performed to analyze the influence of optical parameters on the depth and contrast of the images obtained under 920-970 nm excitation. It was shown that at the same power the excitation wavelength shift reduces the detected signal intensity and the resulting image contrast. Visualization of biological tissue samples using shorter excitation wavelengths 920 and 940 nm also reduces signal-to-background ratio (S/B) of the obtained images. The S/B of the obtained images amounted to 2, 6 and 8 for 920, 940 and 970 nm, respectively. It was demonstrated that pulse-periodic excitation mode is preferable for obtaining high quality luminescent images of biological tissues deep layers and minimize overheating. Short pulse durations (duty cycle 20%) don't result in heating even for 1 W cm(-2) pumping power density and allow obtaining high luminescence intensity, which provides good images quality.
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Optimization of upconversion luminescence excitation mode for deeper in vivo bioimaging without contrast loss or overheating / Pominova, DV [et al.] // Methods and Applications in Fluorescence. - 2020. - 8. - № 2. - 10.1088/2050-6120/ab7782
URI
https://www.doi.org/10.1088/2050-6120/ab7782
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85081129791&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000518678400001
 https://openrepository.mephi.ru/handle/123456789/20402
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