Персона: Лощенов, Виктор Борисович
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Виктор Борисович
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- ПубликацияТолько метаданныеMechanisms of the photodynamic effect with polycationic photosensitizers on the foci of bacterial and oncological diseases(2024) Meerovich, G. A.; Akhlyustina, E. V.; Makarova, E. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
- ПубликацияТолько метаданныеOn the possibility of photodynamic inactivation of tracheobronchial tree pathogenic microbiota using methylene blue (in vitro study)(2022) Tiganova, I. G.; Zulufova, I. D.; Ovchinnikov, R. S.; Solovyev, A. I.; Meerovich, G. A.; Akhlyustina, E. V.; Kozlikina, E. I.; Nekhoroshev, A. V.; Glechik, D. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович© 2022Background: The treatment of patients after mechanical ventilation of lungs suffering from a multi-species infection of the tracheobronchial tree can be complicated. The situation is aggravated in patients with post-intubation tracheal stenosis, where infection plays a leading pathogenetic role in damage to the tracheal wall. As a result of such a pathological process, cicatricial stenosis of the trachea of purulent-inflammatory infectious genesis or infected tracheal stenosis (ITS) may occur. Methods: In this work, we studied the possibility of photodynamic inactivation of pathogenic microbiota typical for patients with ITS using methylene blue (MB) as a photosensitizer. Results: 13 clinical isolates of 8 species of bacteria from 9 patients were susceptible to photodynamic inactivation with MB. 30 μM of MB at a light irradiation dose of 25 J/cm2 and incubation with MB for 15 min allows to completely inactivate bacteria found in the tracheobronchial secretions of patients with ITS. Conclusions: MB retains its optico-physical properties in the range of 3–30 μM and provides effective inactivation of isolated Gram-positive and Gram-negative bacteria, including multi- and pan-resistant to antibiotics.
- ПубликацияТолько метаданныеPHOTO-INDUCED PROCESSES OF IRON OXIDE NANOPARTICLES TO ENHANCE LASER THERAPY фОТОИНдУЦИРОвАННЫЕ пРОЦЕССЫ НАНОчАСТИЦ ОКСИдА ЖЕЛЕЗА дЛя УСИЛЕНИя ЛАЗЕРНОи ТЕРАпИИ(2021) Romanishkin, I. D.; Plotnikova, E. A.; Morozova, N. B.; Wittig, R.; Pominova, D. V.; Loschenov, V. B.; Steiner, R. W.; Ryabova, A. V.; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович; Штайнер, Рудольф Вольфганг; Рябова, Анастасия Владимировна© 2021 Russian Photodynamic Association. All rights reserved.Nanoparticles are used as drug carriers to increase the selectivity and effectiveness of therapy, as well as for combined therapy that utilizes different effects. Iron oxide nanoparticles are promising in this aspect. Due to magnetic properties, they can be used as a contrast agent for magnetic resonance imaging. Also, iron oxide nanoparticles could be coated with a photosensitizer for photodynamic therapy and their laser or magnetic heating can be used for phototherapy. Local enhancement of the electromagnetic field near iron oxide nanoparticles can increase the fluorescence intensity of photosensitizers and the efficiency of singlet oxygen generation. This paper presents the results of a study of iron oxide nanoparticles focused on the photophysical aspects of the formation of “hot spots” under laser irradiation. The photoinduced effects of iron oxide nanoparticles observed in in vitro experiments lead to the rupture of lysosomes. Theoretical modeling showed that the heating of iron oxide nanoparticles with a radius of 35 nm under the action of laser radiation is about 89°C and 19°C for wavelengths of 458 and 561 nm, respectively. Local field enhancement occurs in pairs of nanoparticles of various sizes and strongly depends on the distance between them. The maximum gain is achieved at small distances between nanoparticles. For a dimer of nanoparticles with radii of 10 and 35 nm at a distance of 1 nm, an enhancement factor of two orders of magnitude was obtained. The investigated phenomenon of «hot spots» is in demand for precision therapy, because the photo-induced processes occur at small distances between nanoparticles, in areas of their high accumulation.
- ПубликацияТолько метаданныеTwo diagnostic criteria of optical spectroscopy for bladder tumor detection: Clinical study using 5-ALA induced fluorescence and mathematical modeling(2020) Loshchenov, M.; Amouroux, M.; Daul, C.; Kudashev, B.; Kalyagina, N.; Loschenov, V.; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович© 2020 Elsevier B.V.Background: The study proposes to improve bladder cancer diagnosis by photodynamic diagnosis (PDD) using red-light excitation (632.8 nm) of 5-ALA induced-protoporphyrin IX. Employing 9 patients’ bladders, two types of signals were used to improve diagnostic accuracy for malignancy and we also present numerical modeling of the scattering coefficient to provide biological explanation of the results obtained. Methods: Two modalities of bladder cancer spectral diagnosis are presented: conventional PDD and intensity assessment of the diffusely reflected laser light by fiber-optic spectroscopy. Experiments are done in clinical conditions and as a series of numerical simulations. Results: High-grade cancerous bladder tissues display twice a higher relative fluorescence intensity (mean value 1, n = 9) than healthy (0.39, n = 9), dysplastic (0.44, n = 5) tissues and CIS (0.39, n = 2). The laser back-scattering signal allows to discriminate most effectively high-grade cancerous and dysplastic tissues from normal. Numerical modeling of diffuse reflectance spectra reveals that spectral behavior of the back-scattered light depends on both, nuclear size and nuclear density of tumoral cells. Conclusions: Unlike the fluorescence signal, where its value is higher in the case of pathological tissues, the tendency of the laser signal to, both, decrease or increase in comparison with the signal from normal urothelium, should be perceived as a sign towards neoplasm. Numerical simulation reveals that such a double-analysis at a multiwavelength mode potentially may be used to provide diagnostic accuracy.
- ПубликацияТолько метаданныеPhototheranostics of cervical neoplasms with chlorin e6 photosensitizer(2022) Gilyadova, A.; Ishchenko, A.; Shiryaev, A.; Karpova, R.; Alekseeva, P.; Efendiev, K.; Loschenov, V.; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович© 2022 by the authors. Licensee MDPI, Basel, Switzerland.(1) Purpose: Improving the treatment effectiveness of intraepithelial neoplasia of the cervix associated with human papillomavirus infection, based on the application of the method of photodynamic therapy with simultaneous laser excitation of fluorescence to clarify the boundaries of cervical neoplasms. (2) Methods: Examination and treatment of 52 patients aged 22 to 53 years with morphologically and cytologically confirmed mild to severe intraepithelial cervix neoplasia, preinvasive, micro-invasive, and squamous cell cervix carcinoma. All patients were carriers of human papillomavirus infection. The patients underwent photodynamic therapy with simultaneous laser excitation of fluorescence. The combined use of video and spectral fluorescence diagnostics for cervical neoplasms made it possible to control the photodynamic therapy process at all stages of the procedure. Evaluation of the photodynamic therapy of intraepithelial cervical neoplasms was carried out with colposcopic examination, cytological conclusion, and morphological verification of the biopsy material after the photodynamic therapy course. The success of human papillomavirus therapy was assessed based on the results of the polymerase chain reaction. (3) Results. The possibility of simultaneous spectral fluorescence diagnostics and photodynamic therapy using a laser source with a wavelength of 660 nm has been established, making it possible to assess the fluorescence index in real-time and control the photobleaching of photosensitizers in the irradiated area. The treatment of all 52 patients was successful after the first photodynamic therapy procedure. According to the PCR test of the discharge from the cervical canal, the previously identified HPV types were not observed in 48 patients. Previously identified HPV types were absent after repeated PDT in four patients (CIN III (n = 2), CIS (n = 2)). In 80.8% of patients, regression of the lesion was noted. (4) Conclusions. The high efficiency of photodynamic therapy with intravenous photosensitizer administration of chlorin e6 has been demonstrated both in relation to eradication therapy of human papillomavirus and in relation to the treatment of intraepithelial lesions of the cervix.
- ПубликацияТолько метаданныеNon-invasive high-contrast infrared imaging of blood vessels in biological tissues by the backscattered laser radiation method(2020) Grachev, P.; Moskalev, A.; Efendiev, K.; Loschenov, V.; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович© 2020 Elsevier B.V.The work is devoted to non-invasive visualization of human blood vessels in the near-infrared range by backscattered laser radiation. This article presents an effective approach to infrared imaging using the backscattered laser method. The most optimal range of wavelengths (760–800 nm) for infrared imaging of blood vessels has been determined. The results of the simulation of laser radiation in human skin models by the Monte-Carlo method are presented. A system has been developed for conducting non-invasive infrared imaging of the bloodstream.
- ПубликацияОткрытый доступAttenuation correction technique for fluorescence analysis of biological tissues with significantly different optical properties(2020) Savelieva, T. A.; Kuryanova, M. N.; Akhlyustina, E. V.; Linkov, K. G.; Meerovich, G. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Ахлюстина, Екатерина Витальевна; Меерович, Геннадий Александрович; Лощенов, Виктор БорисовичDuring intraoperative fluorescence navigation to remove various neoplasms and during pharmacokinetic studies of photosensitizers in laboratory animals, in many cases, the ratio of photosensitizer accumulation in the tumor and normal tissue can reach > 10-fold, which inevitably changes their optical properties. At the same time, the tumor formation process causes various metabolic and structural changes at cellular and tissue levels, which lead to changes in optical properties. A hardware-software complex for the spectral-fluorescence studies of the content of fluorochromes in biological tissues with significantly different optical properties was developed, and it was tested on optical phantoms with various concentrations of photosensitizers, absorbers, and scatterers. To correct the influence of optical properties on the photosensitizer concentration analysis by fluorescence spectroscopy, we propose the spectrum-processing algorithm, which combines empirical and theory-based approaches.
- ПубликацияТолько метаданныеSpectroscopic measurement of methylene blue distribution in organs and tissues of hamadryas baboons during oral administration(2021) Pominova, D. V.; Ryabova, A. V.; Rudenko, N. S.; Kulik, O. G.; Kozlikina, E. I.; Efendiev, K. T.; Skobeltsin, A. S.; Loschenov, V. B.; Эфендиев, Канамат Темботович; Скобельцин, Алексей Сергеевич; Лощенов, Виктор Борисович© 2021 by the authors. Licensee MDPI, Basel, Switzerland.New research on Methylene Blue (MB), carried out in 2020, shows that it can be an effective antiviral drug as part of COVID-19 treatment. According to the research findings, MB has potential as a direct antiviral drug for the prevention and treatment of COVID-19 in the first stages of the disease. However, the MB accumulation by various types of tissues, as well as by immune cells, has not been previously studied. Therefore, the objective of this study was to obtain spectral data on the interstitial distribution of the administered drug in endothelial tissues in primates. The data on interstitial MB distribution obtained by spectroscopic measurement at both macro-and microlevels during oral administration to Hamadryas baboon individuals demonstrate that MB accumulates in mucous membranes of gastrointestinal tract and the tissues of the respiratory, cardiovascular, immune, and nervous systems. Additionally, it was found that MB was present in lung and brain myeloid cells in significant concentrations, which makes it potentially useful for protection from autoimmune response (cytokine storm) and as a tool for the correction of immunocompetent cells’ functional state during laser irradiation. Since the cytokine storm starts from monocytic cells during SARS-CoV-2 cellular damage and since tumor-associated macrophages can significantly alter tumor metabolism, accumulation of MB in these cells provides a reason to conclude that the immune response correction in COVID-19 patients and change in macrophages phenotype can be achieved by deactivation of inflammatory macrophages in tissues with MB using laser radiation of red spectral range.
- ПубликацияТолько метаданныеInvestigation of a combined absorption-fluorescence-Raman spectroscopy approach to brain tumor tissue differentiation ex vivo(2022) Romanishkin, I. D.; Savelieva, T. A.; Poletaeva, I. Yu.; Shugai, S. V.; Goryaynov, S. A.; Golbin, D. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор БорисовичIn this work, we used a combined absorption-fluorescence-Raman approach to the optical investigation of brain tissue. The proposed approach allowed taking into account the blood fill, oxygenation, accumulation of fluorescent biomarker, and Raman signal to estimate a degree of malignancy of the tissue. © 2022 IEEE.
- ПубликацияОткрытый доступOptical spectroanalyzer with extended dynamic range for pharmacokinetic investigations of photosensitizers in biotissue Оптическии спектроанализатор с расширенным динамическим диапазоном для фармакокинетических исследовании флуоресцирующих препаратов в биотканях(2019) Linkov, K. G.; Meerovich, G. A.; Akhlyustina, E. V.; Savelieva, T. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович© 2019 Russian Photodynamic Association. All Rights Reserved. Currently, the most promising method for the study of pharmacokinetics of drugs with fluorescent properties is the spectral-fluorescent method. In this article, we propose an algorithm for expanding the dynamic range of the spectrum analyzer by automatically monitoring the maximum spectral density in the recorded fluorescence spectrum and automatically controlled changes in the accumulation time depending on this value, followed by compensation of the output signal with regard to this change, as well as hardware circuit solutions that allow this algorithm. Testing of LESA-01-"Biospeс" spectrum analyzer, upgraded using the proposed approach, was carried out on photosensitizer dispersions based on tetra-3-phenylthiophthalocyanine hydroxyaluminium of various concentrations (from 0.01 mg/l to 50 mg/l), approximately corresponding to the concentrations realized in the process of studying pharmacokinetics in calibration samples and tissues of experimental animals. The proposed solutions that implement the algorithm for recording fluorescence spectra with automatic change of accumulation time depending on the signal level, ensured a significant expansion of the dynamic range of the spectrum analyzer (up to 3.5 orders of magnitude) and improved accuracy in pharmacokinetic studies.