Персона:
Рябова, Анастасия Владимировна

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

Фамилия

Рябова

Имя

Анастасия Владимировна

Полная страница элемента

Результаты поиска

Теперь показываю 1 - 10 из 27

Только метаданные
Fluorescence diagnostics and photodynamic therapy of grain crops pathogenic fungi
(2020) Bikmukhametova, I. R.; Akhlyustina, E. V.; Pominova, D. V.; Ryabova, A. V.; Grachev, P. V.; Makarov, V. I.; Kartabaeva, B. B.; Ахлюстина, Екатерина Витальевна; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Макаров, Владимир Игоревич
© 2020 IEEE.The studies show the dynamics of photosensitizers accumulation in various grain areas during germination and their photodynamic activity against pathogenic microflora (Fusarium, Bipolaris, Alternaria). Four photosensitizers (methylene blue, Chlorin E6, aluminum phthalocyanine in molecular- and nanoform) were used in the work. The accumulation level of methylene blue and aluminum phthalocyanine in molecular form in infected by Alternaria and Fusarium fungi grains was 4-5 times higher than in control on the 4th day. The possibility of pathogenic microflora inactivation using aluminum phthalocyanine was shown.
Только метаданные
IMMUNE TRAINING OF MONOCYTES FROM ATHEROSCLEROTIC PATIENTS IS ASSOCIATED WITH INCREASED INTRACELLULAR CHOLESTEROL LEVEL AND MITOCHONDRIAL ACTIVITY
(2020) Nikiforov, N. G.; Kubekina, M.; Ryabova, A.; Romanishkin, I.; Рябова, Анастасия Владимировна
Только метаданные
Fluorescence lifetime of indocyanine green in molecular and nano-forms in the cellular model of a brain tumor in vitro
(2021) Farrakhova, D. S.; Romanishkin, I. D.; Maklygina, Yu. S.; Yakovlev, D. V.; Ryabova, A. V.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
© 2021 Institute of Physics Publishing. All rights reserved.Any surgical intervention to a central nervous system requires special accuracy and selectivity of the effect on the cancer cells. The application of laser-spectroscopic methods provides a unique opportunity to non-invasively determine the most significant parameters that characterize the tissue states. Moreover, non-invasive state assessment of the brain tumor tissue and surrounding tissues is essential for performing a relapse-free operation. Indocyanine Green (ICG) is a photosensitizer approved for clinical practice and has absorption peak in the near-infrared range corresponding to the spectral transparency window of biological tissue. Also, the aqueous colloidal solution of ICG aggregates was used for spectroscopic properties research in glioma tumor cells. The comparative analysis of ICG in molecular and nanoforms demonstrate the difference between spectral values which allow distinguishing monomers and aggregates in tumor tissue.
Только метаданные
PHOTO-INDUCED PROCESSES OF IRON OXIDE NANOPARTICLES TO ENHANCE LASER THERAPY фОТОИНдУЦИРОвАННЫЕ пРОЦЕССЫ НАНОчАСТИЦ ОКСИдА ЖЕЛЕЗА дЛя УСИЛЕНИя ЛАЗЕРНОи ТЕРАпИИ
(2021) Romanishkin, I. D.; Plotnikova, E. A.; Morozova, N. B.; Wittig, R.; Pominova, D. V.; Loschenov, V. B.; Steiner, R. W.; Ryabova, A. V.; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович; Штайнер, Рудольф Вольфганг; Рябова, Анастасия Владимировна
© 2021 Russian Photodynamic Association. All rights reserved.Nanoparticles are used as drug carriers to increase the selectivity and effectiveness of therapy, as well as for combined therapy that utilizes different effects. Iron oxide nanoparticles are promising in this aspect. Due to magnetic properties, they can be used as a contrast agent for magnetic resonance imaging. Also, iron oxide nanoparticles could be coated with a photosensitizer for photodynamic therapy and their laser or magnetic heating can be used for phototherapy. Local enhancement of the electromagnetic field near iron oxide nanoparticles can increase the fluorescence intensity of photosensitizers and the efficiency of singlet oxygen generation. This paper presents the results of a study of iron oxide nanoparticles focused on the photophysical aspects of the formation of “hot spots” under laser irradiation. The photoinduced effects of iron oxide nanoparticles observed in in vitro experiments lead to the rupture of lysosomes. Theoretical modeling showed that the heating of iron oxide nanoparticles with a radius of 35 nm under the action of laser radiation is about 89°C and 19°C for wavelengths of 458 and 561 nm, respectively. Local field enhancement occurs in pairs of nanoparticles of various sizes and strongly depends on the distance between them. The maximum gain is achieved at small distances between nanoparticles. For a dimer of nanoparticles with radii of 10 and 35 nm at a distance of 1 nm, an enhancement factor of two orders of magnitude was obtained. The investigated phenomenon of «hot spots» is in demand for precision therapy, because the photo-induced processes occur at small distances between nanoparticles, in areas of their high accumulation.
Только метаданные
Detection of Changes in Macrophage Polarization as a Result of 5-Aminolevulinic Acid Photodynamic Therapy Using Fluorescence-Lifetime Imaging Microscopy
(2022) Ryabova, A.; Skobeltsin, A.; Markova, I.; Pominova, D.; Loschenov, V.; Рябова, Анастасия Владимировна; Скобельцин, Алексей Сергеевич; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович
Non-invasive detection of polarized macrophages in tumors is an urgent task in terms of combined antitumor therapy. By analyzing the fluorescence lifetime of the metabolic cofactorsвЂ”the reduced form of nicotinamide adenine dinucleotide (NADH) and flavinsвЂ”differences in cellular metabolism of normal tissue, tumor, inflammatory and anti-inflammatory macrophages were demonstrated. In this work we studied changes in the polarization of macrophages obtained from THP-1 monocytes in response to photodynamic therapy with 5-aminolevulinic acid (ALAвЂ“PDT). Moderate ALAвЂ“PDT in vitro led to changes in M0 macrophages metabolism towards M1 polarization, wherein M1 and M2 macrophages died and were replaced by non-polarized cells. The interstitial distribution of polarized macrophages after ALAвЂ“PDT was studied in a mouse tumor model of grafted Lewis lung carcinoma. In response to ALAвЂ“PDT, there was an increase in the inflammatory macrophages fraction in the tumor node. Metabolic fluorescence-lifetime imaging microscopy (FLIM) was performed for macrophages in vitro and for tumor cryosections. It was shown that analysis of phasor diagrams for the NADH, flavins, and 5-ALA-induced protoporphyrin IX (PpIX) fluorescence lifetime helps to determine the change in metabolism in response to different modes of PDT at the cellular and tissue levels. These data can be used for post-surgery tissue inspection.
Только метаданные
Photodynamic therapy with 5-ALA induced PpIX effect on macrophages polarization
(2022) Ryabova, A. V.; Pominova, D. V.; Skobeltcin, A. S.; Romanishkin, I. D.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор Борисович
In this work, we have carried out the assessment of changes in the polarization of macrophages in response to photodynamic treatment according to the changes in the fluorescence lifetime of respiratory chain enzymes. The analysis of the time-resolved fluorescence of metabolic signatures and PpIX makes it possible to determine the cell metabolism types. © 2022 IEEE.
Только метаданные
Modeling of local field enhancement and laser heating effects in iron oxide nanoparticles
(2022) Pominova, D. V.; Romanishkin, I. D.; Ryabova, A. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
In this work, we have carried out theoretical modeling of heating of iron oxide nanoparticles under the action of laser radiation, modeling of scattering and absorption of exciting laser radiation on iron oxide nanoparticles and their dimers, as well as local field enhancement near individual iron oxide nanoparticles and between two nanoparticles of different sizes forming a dimer. © 2022 IEEE.
Только метаданные
Synthesis and spectroscopic studies of nanostructures based on upconversion nanoparticles coated with a photosensitizer for photodynamic therapy with infrared excitation
(2022) Pominova, D. V.; Proydakova, V. Y.; Romanishkin, I. D.; Ryabova, A. V.; Kuznetsov, S. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
In this work, we studied the processes of energy transfer from upconversion nanoparticles to photosensitizer molecules. The possibility of photodynamic therapy with infrared excitation using synthesized nanostructures has been demonstrated. © 2022 IEEE.
Только метаданные
Theranostic Properties of Crystalline Aluminum Phthalocyanine Nanoparticles as a Photosensitizer
(2022) Makarov, V. I.; Pominova, D. V.; Ryabova, A. V.; Romanishkin, I. D.; Voitova, A. V.; Steiner, R. W.; Loschenov, V. B.; Макаров, Владимир Игоревич; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
The study of phthalocyanines, known photosensitizers, for biomedical applications has been of high research interest for several decades. Of specific interest, nanophotosensitizers are crystalline aluminum phthalocyanine nanoparticles (AlPc NPs). In crystalline form, they are water-insoluble and atoxic, but upon contact with tumors, immune cells, or pathogenic microflora, they change their spectroscopic properties (acquire the ability to fluoresce and become phototoxic), which makes them upcoming agents for selective phototheranostics. Aqueous colloids of crystalline AlPc NPs with a hydrodynamic size of 104 В± 54 nm were obtained using ultrasonic dispersal and centrifugation. Intracellular accumulation and localization of AlPc were studied on HeLa and THP-1 cell cultures and macrophages (M0, M1, M2) by fluorescence microscopy. Crystallinity was assessed by XRD spectroscopy. Time-resolved spectroscopy was used to obtain characteristic fluorescence kinetics of AlPc NPs upon interaction with cell cultures. The photodynamic efficiency and fluorescence quantum yield of AlPc NPs in HeLa and THP-1 cells were evaluated. After entering the cells, AlPc NPs localized in lysosomes and fluorescence corresponding to individual AlPc molecules were observed, as well as destruction of lysosomes and a rapid decrease in fluorescence intensity during photodynamic action. The photodynamic efficiency of AlPc NPs in THP-1 cells was almost 1.8-fold that of the molecular form of AlPc (Photosens). A new mechanism for the occurrence of fluorescence and phototoxicity of AlPc NPs in interaction with cells is proposed.
Только метаданные
Synthesis Strategy of Tetrapyrrolic Photosensitizers for Their Practical Application in Photodynamic Therapy
(2022) Koifman, O. I.; Ageeva, T. A.; Kuzmina, N. S.; Ryabova, A. V.; Pominova, D. V.; Makarov, V. I.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Макаров, Владимир Игоревич; Лощенов, Виктор Борисович