Publication:
Biofunctionalized polyelectrolyte microcapsules encoded with fluorescent semiconductor nanocrystals for highly specific targeting and imaging of cancer cells

Файлы
W2983928622.pdf(2.27 MB)
Дата
2019
Авторы
Baryshnikova, M.
Gomes, F. R.
Alves, F.
Karaulov, A.
Nifontova, G.
Kalenichenko, D.
Nabiev, I.
Нифонтова, Галина Олеговна
Калениченко, Дарья Владимировна
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Научные группы
Организационные подразделения
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Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Аннотация
© 2019 by the authors.Fluorescent semiconductor nanocrystals or quantum dots (QDs) are characterized by unique optical properties, including a high photostability, wide absorption spectrum, and narrow, symmetric fluorescence spectrum. This makes them attractive fluorescent nanolabels for the optical encoding of microcarriers intended for targeted drug delivery, diagnosis, and imaging of transport processes on the body, cellular, and subcellular levels. Incorporation of QDs into carriers in the form of polyelectrolyte microcapsules through layer-by-layer adsorption of oppositely charged polyelectrolyte polymers yields microcapsules with a bright fluorescence signal and adaptable size, structure, and surface characteristics without using organic solvents. The easily modifiable surface of the microcapsules allows for its subsequent functionalization with capture molecules, such as antibodies, which ensures specific and selective interaction with cells, including tumor cells, with the use of the bioconjugation technique developed here. We obtained stable water-soluble nanolabels based on QDs whose surface was modified with polyethylene glycol (PEG) derivatives and determined their colloidal and optical characteristics. The obtained nanocrystals were used to encode polyelectrolyte microcapsules optically. The microcapsule surface was modified with humanized monoclonal antibodies (Abs) recognizing a cancer marker, epidermal growth factor receptor (EGFR). The possibility of effective, specific, and selective delivery of the microcapsules to tumor cells expressing EGFR has been demonstrated. The results show that the QD-encoded polyelectrolyte microcapsules functionalized with monoclonal Abs against EGFR can be used for targeted imaging and diagnosis.
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Biofunctionalized polyelectrolyte microcapsules encoded with fluorescent semiconductor nanocrystals for highly specific targeting and imaging of cancer cells / Baryshnikova, M. [et al.] // Photonics. - 2019. - 6. - № 4. - 10.3390/photonics6040117
URI
https://www.doi.org/10.3390/photonics6040117
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85076626395&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000505522300027
 https://openrepository.mephi.ru/handle/123456789/19053
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