Publication:
Biofunctionalized polyelectrolyte microcapsules encoded with fluorescent semiconductor nanocrystals for highly specific targeting and imaging of cancer cells

Простая страница элемента
dc.contributor.authorBaryshnikova, M.
dc.contributor.authorGomes, F. R.
dc.contributor.authorAlves, F.
dc.contributor.authorKaraulov, A.
dc.contributor.authorNifontova, G.
dc.contributor.authorKalenichenko, D.
dc.contributor.authorNabiev, I.
dc.contributor.authorНифонтова, Галина Олеговна
dc.contributor.authorКалениченко, Дарья Владимировна
dc.contributor.authorНабиев, Игорь Руфаилович
dc.date.accessioned2024-11-21T15:33:53Z
dc.date.available2024-11-21T15:33:53Z
dc.date.issued2019
dc.description.abstract© 2019 by the authors.Fluorescent semiconductor nanocrystals or quantum dots (QDs) are characterized by unique optical properties, including a high photostability, wide absorption spectrum, and narrow, symmetric fluorescence spectrum. This makes them attractive fluorescent nanolabels for the optical encoding of microcarriers intended for targeted drug delivery, diagnosis, and imaging of transport processes on the body, cellular, and subcellular levels. Incorporation of QDs into carriers in the form of polyelectrolyte microcapsules through layer-by-layer adsorption of oppositely charged polyelectrolyte polymers yields microcapsules with a bright fluorescence signal and adaptable size, structure, and surface characteristics without using organic solvents. The easily modifiable surface of the microcapsules allows for its subsequent functionalization with capture molecules, such as antibodies, which ensures specific and selective interaction with cells, including tumor cells, with the use of the bioconjugation technique developed here. We obtained stable water-soluble nanolabels based on QDs whose surface was modified with polyethylene glycol (PEG) derivatives and determined their colloidal and optical characteristics. The obtained nanocrystals were used to encode polyelectrolyte microcapsules optically. The microcapsule surface was modified with humanized monoclonal antibodies (Abs) recognizing a cancer marker, epidermal growth factor receptor (EGFR). The possibility of effective, specific, and selective delivery of the microcapsules to tumor cells expressing EGFR has been demonstrated. The results show that the QD-encoded polyelectrolyte microcapsules functionalized with monoclonal Abs against EGFR can be used for targeted imaging and diagnosis.
dc.identifier.citationBiofunctionalized polyelectrolyte microcapsules encoded with fluorescent semiconductor nanocrystals for highly specific targeting and imaging of cancer cells / Baryshnikova, M. [et al.] // Photonics. - 2019. - 6. - № 4. - 10.3390/photonics6040117
dc.identifier.doi10.3390/photonics6040117
dc.identifier.urihttps://www.doi.org/10.3390/photonics6040117
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85076626395&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000505522300027
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/19053
dc.relation.ispartofPhotonics
dc.titleBiofunctionalized polyelectrolyte microcapsules encoded with fluorescent semiconductor nanocrystals for highly specific targeting and imaging of cancer cells
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue4
oaire.citation.volume6
relation.isAuthorOfPublication37a86d6e-f0fc-46c6-bf24-2383b1570296
relation.isAuthorOfPublication3dcb6007-a35b-4021-9b6c-5c382c02a625
relation.isAuthorOfPublicationa4923e3c-317e-498d-a747-cad6d3054e23
relation.isAuthorOfPublication.latestForDiscovery37a86d6e-f0fc-46c6-bf24-2383b1570296
relation.isOrgUnitOfPublicationc8407a6f-7272-450d-8d99-032352c76b55
relation.isOrgUnitOfPublication.latestForDiscoveryc8407a6f-7272-450d-8d99-032352c76b55
Файлы
Original bundle
Теперь показываю 1 - 1 из 1
Уменьшенное изображение
Name:
W2983928622.pdf
Size:
2.27 MB
Format:
Adobe Portable Document Format
Description:
Download
Коллекции
Публикации