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Олейников, Владимир Александрович

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Олейников

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Владимир Александрович

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Теперь показываю 1 - 10 из 28

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Structure of Supramers Formed by the Amphiphile Biotin-CMG-DOPE
(2020) Zalygin, A.; Solovyeva, D.; Vaskan, I.; Henry, S.; Oleinikov, V.; Олейников, Владимир Александрович
The synthetic function-spacer-lipid (FSL) amphiphile biotin-CMG-DOPE is widely used for delicate ligation of living cells with biotin residues under physiological conditions. Since this molecule has an "apolar-polar-hydrophobic" gemini structure, the supramolecular organization is expected to differ significantly from the classical micelle. Its organization is investigated with experimental methods and molecular dynamics simulations (MDS). Although the linear length of a single biotin-CMG-DOPE molecule is 9.5 nm, the size of the dominant supramer globule is only 14.6 nm. Investigations found that while the DOPE tails form a hydrophobic core, the polar CMG spacer folds back upon itself and predominantly places the biotin reside inside the globule or planar layer. MDS demonstrates that <10 % of biotin residues on the highly water dispersible globules and only 1 % of biotin residues in layer coatings are in an linear conformation and exposing biotin into the aqueous medium. This explains why in biotin-CMG-DOPE apolar biotin residues both in water dispersible globules and coatings on solid surfaces are still capable of interacting with streptavidin.
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Heating ability of elongated magnetic nanoparticles
(2021) Gubanova, E. M.; Usov, N. A.; Oleinikov, V. A.; Губанова, Елизавета Михайловна; Усов, Николай Александрович; Олейников, Владимир Александрович
Low-frequency hysteresis loops and specific absorption rate (SAR) of various assemblies of elongated spheroidal magnetite nanoparticles have been calculated for a range of particle semiaxis ratios a/b = 1.0-3.0. The SAR of a dilute randomly oriented assembly of magnetite nanoparticles in an alternating magnetic field of moderate frequency, f = 300 kHz, and amplitude H0 = 100-200 Oe is shown to decrease significantly with an increase in the aspect ratio of nanoparticles. In addition, there is a narrowing and shift of the intervals of optimal particle diameters towards smaller particle sizes. However, the orientation of a dilute assembly of elongated nanoparticles in a magnetic field leads to an almost twofold increase in SAR at the same frequency and amplitude of the alternating magnetic field, the range of optimal particle diameters remaining unchanged. The effect of the magneto-dipole interaction on the SAR of a dilute assembly of oriented clusters of elongated magnetite nanoparticles has also been investigated depending on the volume fraction of nanoparticles in a cluster. It has been found that the SAR of the assembly of oriented clusters decreases by approximately an order of magnitude with an increase in the volume fraction of nanoparticles in a cluster in the range of 0.04-0.2.
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Ultrastructural 3D Microscopy for Biomedicine: Principles, Applications, and Perspectives
(2024) Mochalov, K. E.; Korzhov, D. S.; Altunina, A. V.; Oleinikov, V. A.; Олейников, Владимир Александрович
Modern biomedical research often requires a three-dimensional microscopic analysis of the ultrastructure of biological objects and materials. Conceptual technical and methodological solutions for three-dimensional structure reconstruction are needed to improve the conventional optical, electron, and probe microscopy methods, which to begin with allow one to obtain two-dimensional images and data. This review discusses the principles and potential applications of such techniques as serial section transmission electron microscopy; techniques based on scanning electron microscopy (SEM) (array tomography, focused ion beam SEM, and serial block-face SEM). 3D analysis techniques based on modern super-resolution optical microscopy methods are described (stochastic optical reconstruction microscopy and stimulated emission depletion microscopy), as well as ultrastructural 3D microscopy methods based on scanning probe microscopy and the feasibility of combining them with optical techniques. A comparative analysis of the advantages and shortcomings of the discussed approaches is performed.
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Nanohybrid Structures Based on Plasmonic or Fluorescent Nanoparticles and Retinal-Containing Proteins
(2020) Zaitsev, S. Y.; Oleinikov, V. A.; Solovyeva, D. O.; Олейников, Владимир Александрович
© 2020, Pleiades Publishing, Ltd.Rhodopsins are light-sensitive membrane proteins enabling transmembrane charge separation (proton pump) on absorption of a light quantum. Bacteriorhodopsin (BR) is a transmembrane protein from halophilic bacteria that belongs to the rhodopsin family. Potential applications of BR are considered so promising that the number of studies devoted to the use of BR itself, its mutant variants, as well as hybrid materials containing BR, in various areas grows steadily. Formation of hybrid structures combining BR with nanoparticles is an essential step in promotion of BR-based devices. However, rapid progress, continuous emergence of new data, as well as challenges of analyzing the entire data require regular reviews of the achievements in this area. This review is devoted to the issues of formation of materials based on hybrids of BR with fluorescent semiconductor nanocrystals (quantum dots) and with noble metal (silver, gold) plasmonic nanoparticles. Recent data on formation of thin (mono-) and thick (multi-) layers from materials containing BR and BR/nanoparticle hybrids are presented.
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Quasistatic hysteresis loops of magnetic nanoparticles in a rotating magnetic field
(2020) Usov, N. A.; Gubanova, E. M.; Epstein, N. B.; Belyaeva, G. A.; Oleinikov, V. A.; Усов, Николай Александрович; Губанова, Елизавета Михайловна; Эпштейн, Наталья Борисовна; Олейников, Владимир Александрович
© 2019Quasistatic hysteresis loops of a single-domain magnetic nanoparticle with uniaxial anisotropy in a rotating magnetic field have been calculated. Magnetic hysteresis is shown to exist only in a limited range of reduced magnetic field amplitudes, 0.5 < h0 < 1, where h0 = H0/Ha, H0 is the amplitude of the rotating magnetic field, Ha being the particle anisotropy field. An analytical formula is obtained for the particle coercive force as a function of the reduced field amplitude. In the domain h0 < 0.5 the magnetization reversal of a particle is impossible, since the final energy barrier exists between the potential wells of the particle for all orientations of applied magnetic field. On the other hand, in the domain h0 > 1 the total energy of the nanoparticle has a single energy minimum, so that there is no magnetic hysteresis. Quasistatic hysteresis loops of a randomly oriented assembly of non interacting nanoparticles in a rotating magnetic field are also obtained.
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Interaction of SiFe Nanoparticles with Epithelial and Lymphoid Cells
(2020) Sharonova, N. V.; Svirshchevskaya, E. V.; Popov, A. A.; Karpov, N. V.; Tikhonovsky, G. V.; Zakharkiv, A. Y.; Timoshenko, V. Y.; Klimentov, S. M.; Oleinikov, V. A.; Попов, Антон Александрович; Тихоновский, Глеб Валерьевич; Захаркив, Анастасия Юрьевна; Тимошенко, Виктор Юрьевич; Климентов, Сергей Михайлович; Олейников, Владимир Александрович
Silicon and silicon-based nanoparticles (SiNP) attract scientific attention due to the biocompatibility and assimilation of silicon by body tissues. Iron-doped SiNP (SiFeNP) allow the use of ferromagnetic properties of iron for NP detection and the possibility of therapeutic application of SiFeNP. The purpose of this work was to analyze the interaction of SiFeNP with epithelial cells (EC) COLO357 and SW620 and human peripheral blood lymphocytes (PBL). SiFeNP were obtained by laser ablation and divided into the NP1 and NP2 fractions of 100 and 150 nm size, respectively. Cytotoxicity, apoptosis induction, reactive oxygen species (ROS) production, and lysosome metabolism were analyzed using in vitro methods. EC were found to efficiently incytosed both types of NPs, which resulted in the increase in the granularity of cells. NP did not cause apoptosis or EC necrosis, but accumulated in lysosomes, which led to a decrease in the membrane potential of lysosomes. In turn, a decrease in the level of EC metabolism led to a gradual (24 h) increase in ROS production by 10-15%. NP1 caused more ROS than NP2, and accumulated more in the EC, which may be the result of a difference in the particle size. SiFeNP did not interact with PBL. Thus, the total cytotoxicity of SiFeNP did not exceed 20%, which is associated with a decrease in lysosome metabolism and insignificant ROS production.
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Peculiarities of biomedical applications of silicon nanoparticles (Review)
(2020) Egorova, A. M.; Klimentov, S. M.; Oleinikov, V. A.; Fayzullaeva, S.; Karpov, N. V.; Zakharkiv, A. Yu.; Tikhonovsky, G. V.; Климентов, Сергей Михайлович; Олейников, Владимир Александрович; Файзуллаева, Сабина Юсуповна; Захаркив, Анастасия Юрьевна; Тихоновский, Глеб Валерьевич
© Published under licence by IOP Publishing Ltd.The review highlights today directions in biomedical applications of silicon nanoparticles for the tasks of early diagnostics and minimum invasive treatment of cancer. It reveals the important issues related to their biocompatibility, toxicity, biodegradation, functionalization and targeting of drugs carried by porous silicon nanocontainers, making stress on theranostic applications of this novel material.
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Исследование локализации микровезикул в клетке методом сканирующей флуоресцентной конфокальной микроспетроскопии
(2024) Белицкая, Е. Д.; Сливка, Е. В.; Олейников, В. А.; Залыгин, А. В.; Олейников, Владимир Александрович; Залыгин, Антон Владленович
В работе представлены первые результаты серии экспериментов по изучению механизма связывания микровезикул с адресной клеткой с помощью метода сканирующей флуоресцентной конфокальной микроскопии. Разработан новый подход для визуализации проникновения микровезикул в клетку.
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Energy transfer mechanisms in nanobiohybrid structures based on quantum dots and photosensitive membrane proteins
(2016) Sizova, S. V.; Oleinikov, V. A.; Bouchonville, N.; Molinari, M.; Samokhvalov, P. S.; Sukhanova, A.; Nabiev, I.; Суханова, Алена Владимировна; Набиев, Игорь Руфаилович; Олейников, Владимир Александрович
The integration of novel nanomaterials with highly functional biological molecules has numerous advanced applications, including optoelectronics, biosensing, imaging, and energy harvesting. This review summarizes recent progress in understanding the mechanisms of energy transfer between semiconductor nanocrystal (so-called quantum dots [QDs]) and photosensitive proteins in heterostructures, such as hybrids of semiconductor nanocrystals with purple membranes containing bacteriorhodopsin (bR) or with photosynthetic reaction centers (RCs). Understanding of these mechanisms should enable prediction of the possible ways to improve the biological function of biomolecules by means of their assembling with QDs and develop novel functional materials with controlled photonic properties and applications. The possible mechanisms of energy transfer from QDs to photochromic biomolecules are discussed and correlated with experimental data. The principles of hybrid structures engineering, donor/acceptor parameters aﬀecting both energy transfer eﬃciency and biological function, and functionality of these hybrid structures are described. New nanobiohybrid materials are shown to have advanced implications for optoelectronics, photonics, and photovoltaics due to the ability of nanocomponents of these materials for eﬃcient energy harvesting, conversion, and transfer of additional energy to Biosystems, thus making them working more eﬃciently.
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Loading efficiency of doxorubicin into the micelle-like structures formed by function-spacer-lipid constructs self-assembly depends on constructs' functional part
(2021) Tarasova, A. R.; Vaskan, I. S.; Troitskaya, P. S.; Bovin, N. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Supramolecular self-assemble systems based on neoglycolipids: Galili-Ad-CMG2-Ad-DOPE, A(type2)-Ad-CMG2-Ad-DOPE are studied here and compared with the well-studied Biotin-CMG2-Ad-DOPE, as well as with their combinations with NH2-CMG2-Ad-DOPE. They are function-spacer-lipid constructs with unique structure that allows them to form micelle-like supramers and be stable, what makes them a potential drug nanocarriers. The structural properties of the obtained supramolecular systems are studied depending on their functional part, and the loading efficiency of doxorubicin into the supramers is determined to reveal the influence of the functional part. The resulting supramers were separated from the unbound molecules by dialysis, the nanoparticles morphology were studied by atomic force microscopy, and the loading efficiency was calculated based on spectrophotometry data. The encapsulation of doxorubicin was confirmed based on changes in the size and shape of the supramers, as well as a decrease in the ratio of unbound molecules. According to the loading efficiency calculations, it was estimated that supramers formed by A(type2)-Ad-CMG2-Ad-DOPE are the most efficient nanocarriers with loading efficiency of 82 %. Supramers formed by NH2-CMG2-Ad-DOPE (no functional part) showed 1.5 times less efficiency. Finally, the least efficient carriers are supramers formed by Biotin-CMG2-Ad-DOPE (14%).