2020, Mochalov, K. E., Solovyova, D. O., Efimov, A. E., Klinov, D. V., Oleinikov, V. A., Олейников, Владимир Александрович

The main limitation of the use of tip-enhanced Raman scattering (TERS) is due to the lack of reliable scanning probes. We present a simple procedure to manufacture TERS probes with highly reproducible characteristics that are reliable and provide sufficient enhancement. The procedure is based on the modification of conventional cantilevers of scanning probe microscopes by plasma etching, followed by the formation of a TERS enhancing region at the apex by deposition of colloidal nanoparticles by dielectrophoresis.

2025, Solovyevа, D., Popadinets, О. Y., Kolpashnikov, I. S., Oleinikov, V. A., Олейников, Владимир Александрович

2020, Egorova, A. M., Klimentov, S. M., Oleinikov, V. A., Fayzullaeva, S., Karpov, N. V., Zakharkiv, A. Yu., Tikhonovsky, G. V., Климентов, Сергей Михайлович, Олейников, Владимир Александрович, Файзуллаева, Сабина Юсуповна, Захаркив, Анастасия Юрьевна, Тихоновский, Глеб Валерьевич

© Published under licence by IOP Publishing Ltd.The review highlights today directions in biomedical applications of silicon nanoparticles for the tasks of early diagnostics and minimum invasive treatment of cancer. It reveals the important issues related to their biocompatibility, toxicity, biodegradation, functionalization and targeting of drugs carried by porous silicon nanocontainers, making stress on theranostic applications of this novel material.

2025, Vaskan, I. S., Piryazev, A. A., Dimitreva, V. A., Oleinikov, V. A., Димитрева, Вероника Алексеевна, Олейников, Владимир Александрович

2023, Dimitreva, V. A., Vaskan ,I. S., Oleinikov, V. A., Zalygin, A. V., Олейников, Владимир Александрович, Залыгин, Антон Владленович, Димитрева, Вероника Алексеевна

2023, Vaskan, I., Dimitreva, V., Petoukhov, M., Oleinikov, V., Zalygin, A., Олейников, Владимир Александрович, Залыгин, Антон Владленович, Димитрева, Вероника Алексеевна

2021, Maslennikov, A. M., Shtykova, E. V., Bovin, N. V., Zalygin, A. V., Oleinikov, V. A., Залыгин, Антон Владленович, Олейников, Владимир Александрович

© 2021 Institute of Physics Publishing. All rights reserved.Neoglycolipids due to their amphiphilic properties exhibit self-assembly in aqueous phases. In high concentrations the liquid crystalline or gel phases may form. So-called soft-material are a subject of interest of many scientists especially as biosensors and wound healing materials. In this study we examine the structure of a quasicrystalline phase of biot-CMG(2)DOPE obtained at the concentration of 150 mg/ml (13wt.%) in PBS. The structural data such as interplanar spacing, order parameter and long-range order were obtained by SAXS, while the changes in chemical structure were studied by Raman spectroscopy. It was also in our interest to examine a correlation between the ionic strength and the self-assembly, so we also studied a similar quasicrystalline phase of the same compound but in a buffer containing CaCl2 at the concentration of 4wt.%. According to SAXS data, FSL-biotin construct formed a complex ordered phase consisting of overlapping latices of different kind. The addition of CaCl2 into PBS resulted in obtaining a more structured system demonstrating cubic-like crystal lattice. Change in peak intensities on Raman spectrums of -C-H- and -C-C- bonds vibrations explained the change in phase properties.

2020, Zalygin, A., Solovyeva, D., Vaskan, I., Henry, S., Oleinikov, V., Олейников, Владимир Александрович

The synthetic function-spacer-lipid (FSL) amphiphile biotin-CMG-DOPE is widely used for delicate ligation of living cells with biotin residues under physiological conditions. Since this molecule has an "apolar-polar-hydrophobic" gemini structure, the supramolecular organization is expected to differ significantly from the classical micelle. Its organization is investigated with experimental methods and molecular dynamics simulations (MDS). Although the linear length of a single biotin-CMG-DOPE molecule is 9.5 nm, the size of the dominant supramer globule is only 14.6 nm. Investigations found that while the DOPE tails form a hydrophobic core, the polar CMG spacer folds back upon itself and predominantly places the biotin reside inside the globule or planar layer. MDS demonstrates that <10 % of biotin residues on the highly water dispersible globules and only 1 % of biotin residues in layer coatings are in an linear conformation and exposing biotin into the aqueous medium. This explains why in biotin-CMG-DOPE apolar biotin residues both in water dispersible globules and coatings on solid surfaces are still capable of interacting with streptavidin.

2023, Vaskan, I. S., Prikhodko, A. T., Petoukhov, M. V., Oleinikov, V. A., Zalygin, A. V., Олейников, Владимир Александрович, Залыгин, Антон Владленович

2020, Zaitsev, S. Y., Oleinikov, V. A., Solovyeva, D. O., Олейников, Владимир Александрович

© 2020, Pleiades Publishing, Ltd.Rhodopsins are light-sensitive membrane proteins enabling transmembrane charge separation (proton pump) on absorption of a light quantum. Bacteriorhodopsin (BR) is a transmembrane protein from halophilic bacteria that belongs to the rhodopsin family. Potential applications of BR are considered so promising that the number of studies devoted to the use of BR itself, its mutant variants, as well as hybrid materials containing BR, in various areas grows steadily. Formation of hybrid structures combining BR with nanoparticles is an essential step in promotion of BR-based devices. However, rapid progress, continuous emergence of new data, as well as challenges of analyzing the entire data require regular reviews of the achievements in this area. This review is devoted to the issues of formation of materials based on hybrids of BR with fluorescent semiconductor nanocrystals (quantum dots) and with noble metal (silver, gold) plasmonic nanoparticles. Recent data on formation of thin (mono-) and thick (multi-) layers from materials containing BR and BR/nanoparticle hybrids are presented.