2023, Скрибицкая, А. В., Короткова, Н. А., Котельникова, П. А., Тихоновский, Г. В., Попов, А. А., Климентов, С. М., Завестовская, И. Н., Кабашин, А. В., Завестовская, Ирина Николаевна, Кабашин, Андрей Викторович, Климентов, Сергей Михайлович, Попов, Антон Александрович, Скрибицкая, Ангелина Вячеславовна, Тихоновский, Глеб Валерьевич

Разработана методика получения нанокомпозитов по типу ядро@оболочка путём поверхностной модификации лазерно-синтезированных наночастиц (НЧ) висмута тетраэтоксисиланом с конечной структурной формулой Bi@SiO2. Показано, что покрытие НЧ Bi оболочкой из SiO2 приводит к образованию сферических наноформуляций с модой размерного распределения 250 – 300 нм. Разработанная методика, позволяющая создавать биосовместимые нанокомпозиты на основе Bi для сенсибилизации мультимодальной тераностики, является новой перспективной альтернативой традиционным методам.

2019, Kondratenko, E. I., Lomteva, N. A., Kasimova, S. K., Yakovenkova, L. A., Timoshenko, V. Yu., Alykova, A. F., Zavestovskaya, I. N., Тимошенко, Виктор Юрьевич, Завестовская, Ирина Николаевна

© Published under licence by IOP Publishing Ltd.The effect of a course application of suspension of porous silicon and gold nanoparticles on the skin of female rats on the level of free radical oxidation and catalase activity in the skin homogenate was studied. MesoPSi samples were formed using the standard method of electrochemical etching of p-type c-Si wafers with a surface orientation of (100) and a specific resistance of 1-5 mOm x cm in a solution of hydrofluoric acid and ethanol (HF (50%): C2H5OH = 1: 1) at an etching current density of 60 mA / cm2 for 1 hour (45 minutes-hour). After this, mesoPSi films were separated from the silicon substrate by a short-term increase in the current density to 600 mA / cm2. Aqueous suspensions of porous silicon nanoparticles with sizes of the order of 100 nm were obtained by mechanical grinding of mesoporous silicon films in water. Aqueous suspensions of gold nanoparticles with an average size of 30-50 nm were obtained by laser ablation of gold targets in deionized water. A suspension of silicon and gold nanoparticles in physiological saline at a concentration of 0.2 mg / ml was applied once a day in the form of a thin layer on a shaved area of the skin of animals (interscapular region) and left to dry completely. Applications were carried out for 10 days. The studied silicon and gold nanoparticles did not change the initial content of malondialdehyde and catalase activity in the skin tissue but contributed to the activation of the rate of spontaneous lipid peroxidation.

2019, Rogov, A., Ryabchikov, Y. V., Geloen, A., Tishchenko, I., Kharin, A. Y., Lysenko, V., Zavestovskaya, I. N., Kabashin, A. V., Timoshenko, V. Y., Завестовская, Ирина Николаевна, Кабашин, Андрей Викторович, Тимошенко, Виктор Юрьевич

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Presenting a safe alternative to conventional compound quantum dots and other functional nanostructures, nanosilicon can offer a series of breakthrough hyperthermia-based therapies under near-infrared, radiofrequency, ultrasound, etc., excitation, but the size range to sensitize these therapies is typically too large (>10 nm) to enable efficient imaging functionality based on photoluminescence properties of quantum-confined excitonic states. Here, it is shown that large Si nanoparticles (NPs) are capable of providing two-photon excited luminescence (TPEL) and second harmonic generation (SHG) responses, much exceeding that of smaller Si NPs, which promises their use as probes for bi-modal nonlinear optical bioimaging. It is finally demonstrated that the combination of TPEL and SHG channels makes possible efficient tracing of both separated Si NPs and their aggregations in different cell compartments, while the resolution of such an approach is enough to obtain 3D images. The obtained bi-modal contrast provides lacking imaging functionality for large Si NPs and promises the development of novel cancer theranostic modalities on their basis.

2019, Lomteva, N. A., Kondratenko, E. I., Kasimova, S. K., Alykova, O. M., Zavestovskaya, I. N., Alykova, A. F., Завестовская, Ирина Николаевна

© 2019 Published under licence by IOP Publishing Ltd. We research memory, exploratory behavior and anxiety in adult rats in an experiment. These parameters were assessed through the use of behavioral assays, namely, the Suok test and passive avoidance learning. These parameters were studied after treatment with extracts of Ginkgo Biloba and salvia. The aqueous extract of salvia and Ginkgo-Biloba contributed to the activation of patterns of exploratory behavior and a decrease of anxiety in animals in the Suok-test. The Ginkgo-Biloba extract stimulates cognitive functions in rats, which is reflected in an improvement in the elaboration of the conditioned reflex and the preservation of the memorable trace after training in the passive avoidance learning.

2023, Савинов, М. С., Грязнова, О. Ю., Тихоновский, Г. В., Попов, А. А., Завестовская, И. Н., Климентов, С. М., Кабашин, А. В., Завестовская, Ирина Николаевна, Кабашин, Андрей Викторович, Климентов, Сергей Михайлович

Исследуется возможность использования наночастиц элементного висмута в качестве сенсибилизаторов радиационной терапии и контрастных агентов компьютерной томографии. Проводится сравнительный анализ рентгеноконтрастных свойств наночастиц висмута с классическими наночастицами золота и нанолистами оксихлорида висмута. Показано, что лазерно-синтезированные наночастицы висмута демонстрируют более высокую эффективность контрастирования рентгеновского излучения по сравнению с традиционными наночастицами золота, а также обладают схожими рентгеноконтрастными свойствами с химически синтезированными аналогами на основе нанолистов оксихлорида висмута. Уникальные физико-химические характеристики в сочетании с высокими рентгеноконтрастными свойствами лазерно-синтезированных наночастиц висмута формируют новую перспективную альтернативу традиционным сенсибилизаторам радиационной тераностики онкологических заболеваний.

2019, Nastulyavichus, A. A., Smirnov, N. A., Khmelnitskiy, R. A., Rudenko, A. A., Kudryashov, S. I., Ivanova, A. K., Yu. , Kharin, A., Zavestovskaya, I. N., Завестовская, Ирина Николаевна

© 2018 Elsevier B.V. High-productivity regime of nanosecond IR-laser ablative generation of silicon colloidal solutions in water for anti-bacterial applications was found in terms of GW/cm2-level laser intensity and scanning velocity by measuring multi-shot ablative mass loss and extinction coefficients of the colloids as sub-linear and third-power intensity functions, respectively. This advantageous regime implies sub-linear mass loss versus laser intensity at the simultaneous third-power yield of nanoparticles, resulting from the subcritical-density, opaque ablative plasma regulating the sample ablation rate and the related plasma-mediated dissociation (dispergation) of the ablation products. In contrast, at higher intensities, there is a drastic increase in mass loss with the corresponding increased yield of (sub) micrometer-sized particles owing to intense plasma-driven expulsion of micro-scale melt droplets and the corresponding saturation of the extinction coefficient of the colloidal solutions because of their dynamic local “self-limiting” effect during the high-rate ablation. The optimal low-intensity regime for Si nanoparticle production demonstrates the monotonous correlated increase of mass loss and extinction coefficient in terms of increasing laser scanning velocity, indicating the diminished cumulative effects. Surface coatings prepared from the generated Si nanoparticles exhibit minor surface oxidation, as acquired as their elemental composition via energy-dispersive X-ray spectroscopy, making their contact angle for water droplets (≈51°) close to that of bare Si wafer (≈58°) with its nanometer-thick native oxide layer. Owing to good wetting, the nanoparticle-based surface coatings show strong antibacterial response regarding Gram-negative Pseudomonas auereginosa bacteria even despite their minor oxidative passivation.

2019, Petriev, V. M., Tishchenko, V. K., Smoryzanova, O. A., Zavestovskaya, I. N., Postnov, A. A., Завестовская, Ирина Николаевна

© 2019, Allerton Press, Inc.This paper concerns the study of pharmacokinetic properties of rhenium-188 and gallium-68 bound to a ligand based on tetraphosphonic acid (188 Re,68 Ga-EDTMP). The pharmacokinetics of 188 Re,68 Ga-EDTMP is studied using outbred rats after intravenous injection of the preparation; the results are compared with the properties of 188 Re-EDTMP and 68 Ga-EDTMP. Rapid and selective activity accumulation in bone tissue is observed. The peak specific contents of 68 Ga and 188 Re in the femoral bone are 6.85%/g and 2.76%/g, respectively. Throughout the study, the activity of radionuclides in bone tissue was higher than in blood and most soft tissue organs. 188 Re,68 Ga-EDTMP was excreted via the urinary tract. Increased activity is recorded in the thyroid gland. Other organs and tissues are characterized by low contents of the preparation. After intravenous injection of 188 Re-EDTMP and 68 Ga-EDTMP, the activity of 188 Re and 68 Ga is lower in comparison with 188 Re,68 Ga-EDTMP. Thus,188 Re,68 Ga-EDTMP is a promising preparation for theranostics of bone metastases.

2019, Wriedt, T., Lopushenko, I. V., Zavestovskaya, I. N., Завестовская, Ирина Николаевна

© 2019, Allerton Press, Inc.Using the modified discrete sources method, the problem of calculating plasmon spectra of the characteristic energy loss of electrons during the interaction with silver nanoparticles from 2 to 8 nm in size is solved. The calculated spectra are compared with the experimental data confirming the significant spatial dispersion effect. The importance of the consideration of this factor when analyzing the properties of plasmonic nanoparticles for applications in biosensorics and biomedicine is discussed.

2019, Tischenko, V. K., Mikhailovskaya, A. A., Popov, A. A., Tselikov, G., Petriev, V. M., Deyev, S. M., Timoshenko, V. Y., Prasad, P. N., Zavestovskaya, I. N., Kabashin, A. V., Деев, Сергей Михайлович, Тимошенко, Виктор Юрьевич, Завестовская, Ирина Николаевна, Кабашин, Андрей Викторович

© 2019, The Author(s). Nuclear nanomedicine, with its targeting ability and heavily loading capacity, along with its enhanced retention to avoid rapid clearance as faced with molecular radiopharmaceuticals, provides unique opportunities to treat tumors and metastasis. Despite these promises, this field has seen limited activities, primarily because of a lack of suitable nanocarriers, which are safe, excretable and have favorable pharmacokinetics to efficiently deliver and retain radionuclides in a tumor. Here, we introduce biodegradable laser-synthesized Si nanoparticles having round shape, controllable low-dispersion size, and being free of any toxic impurities, as highly suitable carriers of therapeutic 188 Re radionuclide. The conjugation of the polyethylene glycol-coated Si nanoparticles with radioactive 188 Re takes merely 1 hour, compared to its half-life of 17 hours. When intravenously administered in a Wistar rat model, the conjugates demonstrate free circulation in the blood stream to reach all organs and target tumors, which is radically in contrast with that of the 188 Re salt that mostly accumulates in the thyroid gland. We also show that the nanoparticles ensure excellent retention of 188 Re in tumor, not possible with the salt, which enables one to maximize the therapeutic effect, as well as exhibit a complete time-delayed conjugate bioelimination. Finally, our tests on rat survival demonstrate excellent therapeutic effect (72% survival compared to 0% of the control group). Combined with a series of imaging and therapeutic functionalities based on unique intrinsic properties of Si nanoparticles, the proposed biodegradable complex promises a major advancement in nuclear nanomedicine.

2019, Singh, A., Swihart, M. T., Kabashin, A. V., Zavestovskaya, I. N., Prasad, P. N., Кабашин, Андрей Викторович, Завестовская, Ирина Николаевна

Copyright © 2019 American Chemical Society.This review describes promising laser-based approaches to produce silicon nanostructures, including laser ablation of solid Si targets in residual gases and liquids and laser pyrolysis of silane. These methods are different from, and complementary to, widely used porous silicon technology and alternative synthesis routes. One can use these methods to make stable colloidal dispersions of silicon nanoparticles in both organic and aqueous media, which are suitable for a multitude of applications across the important fields of energy and healthcare. Size tailoring allows production of Si quantum dots with efficient photoluminescence that can be tuned across a broad spectral range from the visible to near-IR by varying particle size and surface functionalization. These nanoparticles can also be integrated with other nanomaterials to make multifunctional composites incorporating magnetic and/or plasmonic components. In the energy domain, this review highlights applications to photovoltaics and photodetectors, nanostructured silicon anodes for lithium ion batteries, and hydrogen generation from water. Application to nanobiophotonics and nanomedicine profits from the excellent biocompatibility and biodegradability of nanosilicon. These applications encompass several types of bioimaging and various therapies, including photodynamic therapy, RF thermal therapy, and radiotherapy. The review concludes with a discussion of challenges and opportunities in the applications of laser-processed nanosilicon.