Publication:
Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads

Файлы
W3001417001.pdf(3.3 MB)
Дата
2020
Авторы
Kage, D.
Hoffmann, K.
Sukhanova, A.
Resch-Genger, U.
Nifontova, G.
Krivenkov, V.
Nabiev, I.
Нифонтова, Галина Олеговна
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Организационные подразделения
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Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Аннотация
© 2020, The Author(s).Semiconductor quantum dots (QDs) embedded into polymer microbeads are known to be very attractive emitters for spectral multiplexing and colour encoding. Their luminescence lifetimes or decay kinetics have been, however, rarely exploited as encoding parameter, although they cover time ranges which are not easily accessible with other luminophores. We demonstrate here the potential of QDs made from II/VI semiconductors with luminescence lifetimes of several 10 ns to expand the lifetime range of organic encoding luminophores in multiplexing applications using time-resolved flow cytometry (LT-FCM). For this purpose, two different types of QD-loaded beads were prepared and characterized by photoluminescence measurements on the ensemble level and by single-particle confocal laser scanning microscopy. Subsequently, these lifetime-encoded microbeads were combined with dye-encoded microparticles in systematic studies to demonstrate the potential of these QDs to increase the number of lifetime codes for lifetime multiplexing and combined multiplexing in the time and colour domain (tempo-spectral multiplexing). These studies were done with a recently developed novel luminescence lifetime flow cytometer (LT-FCM setup) operating in the time-domain, that presents an alternative to reports on phase-sensitive lifetime detection in flow cytometry.
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Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads / Kage, D. [et al.] // Scientific Reports. - 2020. - 10. - № 1. - 10.1038/s41598-019-56938-2
URI
https://www.doi.org/10.1038/s41598-019-56938-2
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85078242474&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000562134000014
 https://openrepository.mephi.ru/handle/123456789/20142
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