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Поминова, Дарья Вячеславовна

Организационные подразделения

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Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Поминова

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Дарья Вячеславовна

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NaGdF4:Yb, Er, Tm Upconversion Nanoparticles for Bioimaging in Shortwave-Infrared Range: Study of Energy Transfer Processes and Composition Optimization
(2024) Pominova, D.; Proydakova, V.; Romanishkin, I.; Ryabova, A.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна
Upconversion nanoparticles are promising for many applications. For triple-doped nanoparticles (NPs), the luminescence intensity shows a non-linear dependence on the rare-earth ion concentration, making it difficult to obtain bright phosphors with high energy output. We investigated the energy transfer processes in ОІ-NaGdF4:Yb-Er-Tm NPs and considered strategies for increasing the thulium luminescence intensity, in particular, the use of coreвЂ“shell structures. The luminescence spectra were analyzed in the short-wavelength infrared (SWIR) and visible (VIS) regions. The Er3+ and Tm3+ luminescence lifetimes in the VIS region were measured to study the energy transfer processes between the active ions. The quenching of the Tm3+ luminescence in the SWIR region was observed. However, both Er3+ and Tm3+ luminescence bands were observed in the VIS range. We attribute these effects to energy transfer between Tm3+ 3F4 в†’ 3H6 and Er3+ 4I13/2 в†’ 4I9/2, which occurs due to overlap of Er3+ and Tm3+ luminescence bands, and also to competition between Er3+ and Tm3+ for energy transfer from Yb3+. For coreвЂ“shell NPs, when Tm3+ and Er3+ are separated into adjacent layers, quenching cannot be avoided, likely due to the mutual diffusion of ions during shell synthesis. The most optimal strategy to obtain luminescence in the SWIR range is to use an inert intermediate shell between the layers containing Tm3+ and Er3+.
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The use of methylene blue to control the tumor oxygenation level
(2024) Pominova, D.; Ryabova, A.; Skobeltsin, A.; Markova, I.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Скобельцин, Алексей Сергеевич
Hypoxia is a characteristic feature of many tumors. It promotes tumor proliferation, metastasis, and invasion and can reduce the effectiveness of many types of cancer treatment. The aim of this study was to investigate the pharmacokinetics of methylene blue (MB) and its impact on the tumor oxygenation level at mouse Lewis lung carcinoma (LLC) model using spectroscopic methods. The pharmacokinetics of MB were studied qualitatively and quantitatively using video fluorescence imaging and fluorescence spectroscopy. The degree of hemoglobin oxygenation in vivo was examined by calculating hemoglobin optical absorption from the measured diffuse reflectance spectra. The distribution of MB fluorescence and the lifetime of NADH were analyzed using laser scanning microscopy and fluorescence lifetime imaging microscopy (FLIM) to assess cellular metabolism. After intravenous administration of MB at 10ў??20 mg/kg, it quickly transitioned in the tumor to a colorless leucomethylene blue, with maximum accumulation in the tumor occurring after 5ў??10 minutes. A concentration of 10 mg/kg resulted in a relative increase of the tumor oxygenation level for small tumors (volume 50ў??75 mm3) and normal tissue 120 minutes after the introduction of MB. A shift in tumor metabolism towards oxidative phosphorylation (according to the lifetime of the NADH coenzyme) was measured using FLIM method after intravenous administration of 10 mg/kg of MB. Intravenous administration of MB at 20 mg/kg results in a long-term decrease in oxygenation, which persisted for at least 120 minutes after the administration and did not return to its initial level. Administration of MB at 10 mg/kg shown to increase tumor oxygenation level, potentially leading to more effective antitumor therapy. However, at higher doses (20 mg/kg), MB may cause long-term decrease in oxygenation.
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Spectral properties of crystalline aluminum phthalocyanine nanoparticles and the possibility of their use in biophotonics
(2024) Makarov, V. I.; Pominova, D. V.; Ryabova, A. V.; Loschenov, V. B.; Макаров, Владимир Игоревич; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
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Fluorescence diagnostics and photodynamic therapy of grain crops pathogenic fungi
(2020) Bikmukhametova, I. R.; Akhlyustina, E. V.; Pominova, D. V.; Ryabova, A. V.; Grachev, P. V.; Makarov, V. I.; Kartabaeva, B. B.; Ахлюстина, Екатерина Витальевна; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Макаров, Владимир Игоревич
© 2020 IEEE.The studies show the dynamics of photosensitizers accumulation in various grain areas during germination and their photodynamic activity against pathogenic microflora (Fusarium, Bipolaris, Alternaria). Four photosensitizers (methylene blue, Chlorin E6, aluminum phthalocyanine in molecular- and nanoform) were used in the work. The accumulation level of methylene blue and aluminum phthalocyanine in molecular form in infected by Alternaria and Fusarium fungi grains was 4-5 times higher than in control on the 4th day. The possibility of pathogenic microflora inactivation using aluminum phthalocyanine was shown.
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PHOTO-INDUCED PROCESSES OF IRON OXIDE NANOPARTICLES TO ENHANCE LASER THERAPY фОТОИНдУЦИРОвАННЫЕ пРОЦЕССЫ НАНОчАСТИЦ ОКСИдА ЖЕЛЕЗА дЛя УСИЛЕНИя ЛАЗЕРНОи ТЕРАпИИ
(2021) Romanishkin, I. D.; Plotnikova, E. A.; Morozova, N. B.; Wittig, R.; Pominova, D. V.; Loschenov, V. B.; Steiner, R. W.; Ryabova, A. V.; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович; Штайнер, Рудольф Вольфганг; Рябова, Анастасия Владимировна
© 2021 Russian Photodynamic Association. All rights reserved.Nanoparticles are used as drug carriers to increase the selectivity and effectiveness of therapy, as well as for combined therapy that utilizes different effects. Iron oxide nanoparticles are promising in this aspect. Due to magnetic properties, they can be used as a contrast agent for magnetic resonance imaging. Also, iron oxide nanoparticles could be coated with a photosensitizer for photodynamic therapy and their laser or magnetic heating can be used for phototherapy. Local enhancement of the electromagnetic field near iron oxide nanoparticles can increase the fluorescence intensity of photosensitizers and the efficiency of singlet oxygen generation. This paper presents the results of a study of iron oxide nanoparticles focused on the photophysical aspects of the formation of “hot spots” under laser irradiation. The photoinduced effects of iron oxide nanoparticles observed in in vitro experiments lead to the rupture of lysosomes. Theoretical modeling showed that the heating of iron oxide nanoparticles with a radius of 35 nm under the action of laser radiation is about 89°C and 19°C for wavelengths of 458 and 561 nm, respectively. Local field enhancement occurs in pairs of nanoparticles of various sizes and strongly depends on the distance between them. The maximum gain is achieved at small distances between nanoparticles. For a dimer of nanoparticles with radii of 10 and 35 nm at a distance of 1 nm, an enhancement factor of two orders of magnitude was obtained. The investigated phenomenon of «hot spots» is in demand for precision therapy, because the photo-induced processes occur at small distances between nanoparticles, in areas of their high accumulation.
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Detection of Changes in Macrophage Polarization as a Result of 5-Aminolevulinic Acid Photodynamic Therapy Using Fluorescence-Lifetime Imaging Microscopy
(2022) Ryabova, A.; Skobeltsin, A.; Markova, I.; Pominova, D.; Loschenov, V.; Рябова, Анастасия Владимировна; Скобельцин, Алексей Сергеевич; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович
Non-invasive detection of polarized macrophages in tumors is an urgent task in terms of combined antitumor therapy. By analyzing the fluorescence lifetime of the metabolic cofactorsвЂ”the reduced form of nicotinamide adenine dinucleotide (NADH) and flavinsвЂ”differences in cellular metabolism of normal tissue, tumor, inflammatory and anti-inflammatory macrophages were demonstrated. In this work we studied changes in the polarization of macrophages obtained from THP-1 monocytes in response to photodynamic therapy with 5-aminolevulinic acid (ALAвЂ“PDT). Moderate ALAвЂ“PDT in vitro led to changes in M0 macrophages metabolism towards M1 polarization, wherein M1 and M2 macrophages died and were replaced by non-polarized cells. The interstitial distribution of polarized macrophages after ALAвЂ“PDT was studied in a mouse tumor model of grafted Lewis lung carcinoma. In response to ALAвЂ“PDT, there was an increase in the inflammatory macrophages fraction in the tumor node. Metabolic fluorescence-lifetime imaging microscopy (FLIM) was performed for macrophages in vitro and for tumor cryosections. It was shown that analysis of phasor diagrams for the NADH, flavins, and 5-ALA-induced protoporphyrin IX (PpIX) fluorescence lifetime helps to determine the change in metabolism in response to different modes of PDT at the cellular and tissue levels. These data can be used for post-surgery tissue inspection.
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Photodynamic therapy with 5-ALA induced PpIX effect on macrophages polarization
(2022) Ryabova, A. V.; Pominova, D. V.; Skobeltcin, A. S.; Romanishkin, I. D.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор Борисович
In this work, we have carried out the assessment of changes in the polarization of macrophages in response to photodynamic treatment according to the changes in the fluorescence lifetime of respiratory chain enzymes. The analysis of the time-resolved fluorescence of metabolic signatures and PpIX makes it possible to determine the cell metabolism types. © 2022 IEEE.
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Modeling of local field enhancement and laser heating effects in iron oxide nanoparticles
(2022) Pominova, D. V.; Romanishkin, I. D.; Ryabova, A. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
In this work, we have carried out theoretical modeling of heating of iron oxide nanoparticles under the action of laser radiation, modeling of scattering and absorption of exciting laser radiation on iron oxide nanoparticles and their dimers, as well as local field enhancement near individual iron oxide nanoparticles and between two nanoparticles of different sizes forming a dimer. © 2022 IEEE.
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Synthesis and spectroscopic studies of nanostructures based on upconversion nanoparticles coated with a photosensitizer for photodynamic therapy with infrared excitation
(2022) Pominova, D. V.; Proydakova, V. Y.; Romanishkin, I. D.; Ryabova, A. V.; Kuznetsov, S. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
In this work, we studied the processes of energy transfer from upconversion nanoparticles to photosensitizer molecules. The possibility of photodynamic therapy with infrared excitation using synthesized nanostructures has been demonstrated. © 2022 IEEE.
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Theranostic Properties of Crystalline Aluminum Phthalocyanine Nanoparticles as a Photosensitizer
(2022) Makarov, V. I.; Pominova, D. V.; Ryabova, A. V.; Romanishkin, I. D.; Voitova, A. V.; Steiner, R. W.; Loschenov, V. B.; Макаров, Владимир Игоревич; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
The study of phthalocyanines, known photosensitizers, for biomedical applications has been of high research interest for several decades. Of specific interest, nanophotosensitizers are crystalline aluminum phthalocyanine nanoparticles (AlPc NPs). In crystalline form, they are water-insoluble and atoxic, but upon contact with tumors, immune cells, or pathogenic microflora, they change their spectroscopic properties (acquire the ability to fluoresce and become phototoxic), which makes them upcoming agents for selective phototheranostics. Aqueous colloids of crystalline AlPc NPs with a hydrodynamic size of 104 В± 54 nm were obtained using ultrasonic dispersal and centrifugation. Intracellular accumulation and localization of AlPc were studied on HeLa and THP-1 cell cultures and macrophages (M0, M1, M2) by fluorescence microscopy. Crystallinity was assessed by XRD spectroscopy. Time-resolved spectroscopy was used to obtain characteristic fluorescence kinetics of AlPc NPs upon interaction with cell cultures. The photodynamic efficiency and fluorescence quantum yield of AlPc NPs in HeLa and THP-1 cells were evaluated. After entering the cells, AlPc NPs localized in lysosomes and fluorescence corresponding to individual AlPc molecules were observed, as well as destruction of lysosomes and a rapid decrease in fluorescence intensity during photodynamic action. The photodynamic efficiency of AlPc NPs in THP-1 cells was almost 1.8-fold that of the molecular form of AlPc (Photosens). A new mechanism for the occurrence of fluorescence and phototoxicity of AlPc NPs in interaction with cells is proposed.