2019, Linkov, K. G., Meerovich, G. A., Akhlyustina, E. V., Savelieva, T. A., Loschenov, V. B., Меерович, Геннадий Александрович, Ахлюстина, Екатерина Витальевна, Савельева, Татьяна Александровна, Лощенов, Виктор Борисович

© 2019 Russian Photodynamic Association. All Rights Reserved. Currently, the most promising method for the study of pharmacokinetics of drugs with fluorescent properties is the spectral-fluorescent method. In this article, we propose an algorithm for expanding the dynamic range of the spectrum analyzer by automatically monitoring the maximum spectral density in the recorded fluorescence spectrum and automatically controlled changes in the accumulation time depending on this value, followed by compensation of the output signal with regard to this change, as well as hardware circuit solutions that allow this algorithm. Testing of LESA-01-"Biospeс" spectrum analyzer, upgraded using the proposed approach, was carried out on photosensitizer dispersions based on tetra-3-phenylthiophthalocyanine hydroxyaluminium of various concentrations (from 0.01 mg/l to 50 mg/l), approximately corresponding to the concentrations realized in the process of studying pharmacokinetics in calibration samples and tissues of experimental animals. The proposed solutions that implement the algorithm for recording fluorescence spectra with automatic change of accumulation time depending on the signal level, ensured a significant expansion of the dynamic range of the spectrum analyzer (up to 3.5 orders of magnitude) and improved accuracy in pharmacokinetic studies.

2019, Tiganova, I. G., Makarova, E. A., Philipova, N. I., Romanishkin, I. D., Akhlyustina, E. V., Meerovich, G. A., Loschenov, V. B., Ахлюстина, Екатерина Витальевна, Меерович, Геннадий Александрович, Лощенов, Виктор Борисович

© 2019 Published under licence by IOP Publishing Ltd. Polycationic derivatives of synthetic bacteriochlorin with absorption in the near infrared range are promising for the creation of photosensitizers (PS) for antimicrobial photodynamic therapy. In the present work, the photophysical and antibacterial properties of PS based on tetracationic derivatives of synthetic bacteriochlorins: hydrophilic 3-Py 4 BC(EtBr) 4 Br4 in aqueous solution and amphiphilic 3-Py 4 BCHP 4 Br 4 in the dispersion of Kolliphor ELP were studied. Analysis of absorption and fluorescence in a wide range of concentrations has demonstrated low aggregation of the PS over the entire range. A high efficiency of photodynamic inactivation of Gram-positive Staphylococcus aureus and Gramnegative Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii bacteria was observed.