Персона: Ахлюстина, Екатерина Витальевна
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Екатерина Витальевна
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- ПубликацияТолько метаданныеPhotodynamic inactivation of bacteria: Why it is not enough to excite a photosensitizer(2023) Meerovich, G. A.; Akhlyustina, E. V.; Romanishkin, I. D.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
- ПубликацияТолько метаданныеThe Impact of COVID-19 Infection on Lung Cancer Stem Cells and Photodynamic Therapy as a Potential Way to Combat Both Pathologies(2024) Kogan, E. A.; Meerovich, G. A.; Avraamova, S. T.; Akhlyustina, E. V.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна
- ПубликацияТолько метаданныеSTUdy OF ENERGy TRANSFER pROCESSES BETWEEN RARE EARTh IONS ANd phOTOSENSITIZER mOLECULES FOR phOTOdyNAmIC ThERApy WITh IR-EXCITATION ИССЛЕдОвАНИЕ пРОЦЕССОв пЕРЕдАчИ ЭНЕРГИИ мЕЖдУ РЕдКОЗЕмЕЛЬНЫмИ ИОНАмИ И мОЛЕКУЛАмИ фОТОСЕНСИБИЛИЗАТОРОв дЛя ЗАдАч фОТОдИНАмИчЕСКОи ТЕРАпИИ С вОЗБУЖдЕНИЕм в ИК-дИАпАЗОНЕ(2021) Proydakova, V. Y.; Romanishkin, I. D.; Kuznetsov, S. V.; Lukyanets, E. A.; Pominova, D. V.; Bogatova, A. S.; Akhlyustina, E. V.; Saveleva, T. A.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Ахлюстина, Екатерина Витальевна; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович© 2021 Russian Photodynamic Association. All rights reserved.Today, photodynamic therapy is one of the most promising minimally invasive methods of treatment of various diseases, including cancer. The main limitation of this method is the insufficient penetration into the tissue of laser radiation used to activate photosensitizer molecules,which makes it difficult to carry out therapy in the treatment of large or deep-seated tumors. In this regard, there is a great interest in thedevelopment of new strategies for photodynamic therapy using infrared radiation for excitation, the wavelengths of which fall into the “transparencywindow” of biological tissues. In this work, it was proposed to use upconversion NaGdF4:Yb:Er nanoparticles (UCNP), which absorbinfrared excitation and serve as a donor that transfers energy to the photosensitizer. Photosens and phthalosens were chosen as the mostpromising photosensitizers for the study. The aim of this work was to study the energy transfer processes between upconversion nanoparticlesdoped with rare-earth ions and photosensitizer molecules. in order to excite photosensitizers with IR radiation and carry out photodynamictherapy of deep-seated neoplasms. Using spectroscopic and time-resolved methods, it has been demonstrated that there is an efficientenergy transfer between upconversion particles and photosensitizers phthalosens and photosens. The calculated efficiency of energy transferby the Foerster mechanism was 41% for the UCNP + photosens system and 69% for the UCNP + phthalosens system. It has been experimentallyand theoretically proved that there is a binding of photosensitizer molecules with UCNP by means of surfactants, leading to a reductionin the distance between them, due to which effective nonradiative energy transfer is realized. The generation of singlet oxygen by the phthalosensphotosensitizer upon excitation by means of energy transfer from UCNP, excited at 980 nm wavelength of, has been demonstrated.
- ПубликацияТолько метаданныеFluorescence diagnostics and photodynamic therapy of grain crops pathogenic fungi(2020) Bikmukhametova, I. R.; Akhlyustina, E. V.; Pominova, D. V.; Ryabova, A. V.; Grachev, P. V.; Makarov, V. I.; Kartabaeva, B. B.; Ахлюстина, Екатерина Витальевна; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Макаров, Владимир Игоревич© 2020 IEEE.The studies show the dynamics of photosensitizers accumulation in various grain areas during germination and their photodynamic activity against pathogenic microflora (Fusarium, Bipolaris, Alternaria). Four photosensitizers (methylene blue, Chlorin E6, aluminum phthalocyanine in molecular- and nanoform) were used in the work. The accumulation level of methylene blue and aluminum phthalocyanine in molecular form in infected by Alternaria and Fusarium fungi grains was 4-5 times higher than in control on the 4th day. The possibility of pathogenic microflora inactivation using aluminum phthalocyanine was shown.
- ПубликацияТолько метаданныеMechanisms of the photodynamic effect with polycationic photosensitizers on the foci of bacterial and oncological diseases(2024) Meerovich, G. A.; Akhlyustina, E. V.; Makarova, E. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
- ПубликацияТолько метаданныеOn the possibility of photodynamic inactivation of tracheobronchial tree pathogenic microbiota using methylene blue (in vitro study)(2022) Tiganova, I. G.; Zulufova, I. D.; Ovchinnikov, R. S.; Solovyev, A. I.; Meerovich, G. A.; Akhlyustina, E. V.; Kozlikina, E. I.; Nekhoroshev, A. V.; Glechik, D. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович© 2022Background: The treatment of patients after mechanical ventilation of lungs suffering from a multi-species infection of the tracheobronchial tree can be complicated. The situation is aggravated in patients with post-intubation tracheal stenosis, where infection plays a leading pathogenetic role in damage to the tracheal wall. As a result of such a pathological process, cicatricial stenosis of the trachea of purulent-inflammatory infectious genesis or infected tracheal stenosis (ITS) may occur. Methods: In this work, we studied the possibility of photodynamic inactivation of pathogenic microbiota typical for patients with ITS using methylene blue (MB) as a photosensitizer. Results: 13 clinical isolates of 8 species of bacteria from 9 patients were susceptible to photodynamic inactivation with MB. 30 μM of MB at a light irradiation dose of 25 J/cm2 and incubation with MB for 15 min allows to completely inactivate bacteria found in the tracheobronchial secretions of patients with ITS. Conclusions: MB retains its optico-physical properties in the range of 3–30 μM and provides effective inactivation of isolated Gram-positive and Gram-negative bacteria, including multi- and pan-resistant to antibiotics.
- ПубликацияТолько метаданныеAntitumor activity of photodynamic therapy with tetracationic derivative of synthetic bacteriochlorin in spheroid culture of liver and colon cancer cells(2022) Karshieva, S. S.; Glinskaya, E. G.; Dalina, A. A.; Akhlyustina, E. V.; Mironov, V. A.; Meerovich, G. A.; Kogan, E. A.; Koudan, E. V.; Ахлюстина, Екатерина Витальевна; Миронов, Владимир Александрович; Меерович, Геннадий Александрович; Кудан, Елизавета ВалерьевнаEfficient screening of photosensitizers (PS) as well as studying their photodynamic activity, especially PS excited in the near-infrared region, require informative in vitro models to adequately reflect the architecture, thickness, and intercellular interactions in tumors. In our study, we used spheroids formed from human colon cancer HCT-116 cells and liver cancer Huh7 cells to assess the phototoxicity of a new PS based on tetracationic derivative of synthetic bacteriochlorin (BC4). We optimized conditions for the irradiation regime based on the kinetics of BC4 accumulation in spheroids and kinetics of spheroid growth. Although PS accumulated more efficiently in HCT-116 cells, characterized by more aggressive growth and high proliferative potential, they were less susceptible to the photodynamic therapy (PDT) compared to the slower growing Huh7 cells. We also showed that 3D models of spheroids were less sensitive to BC4 than conventional 2D cultures with relatively identical kinetics of drug accumulation. Our findings suggest that BC4 is a perspective agent for photodynamic therapy against cancer cells. Copyright © 2022 Elsevier B.V. All rights reserved.
- ПубликацияТолько метаданныеСпектрально-оптические свойства катионных фотосенсибилизаторов на основе производных бактериохлорина для антибактериальной фототераностики в ближнем ИК-диапазоне(2021) Ахлюстина, Е. В.; Ахлюстина, Екатерина Витальевна; Лощенов Виктор БорисовичНа сегодняшний день разработка и исследование новых катионных фотосенсибилизаторов, таких как катионные бактериохлорины, являются актульными задачами, поскольку они показали более высокую чем нейтральные и анионные фотосенсибилизаторы в отношении инактивации бактерий. В частности, необходимо подобрать наиболее эффективные фотосенсибилизаторы из ряда синтезированных производных катионных бактерихлоринов для задач антибактериальной фотодинамический терапии. Для решения этой задачи были исследованы их фотофизические свойства, такие как поглощение, флуоресценция, фотобличинг(фотодеградация), времена жизни флуоресценции, агрегация. Другой важной задачей является эквивалентное облучение образцов для микробиологических исследований. В работе была проведена оценка доли поглощенной энергии в тонких слоях при работах in vitro в зависимости от источника облучения и характеристик фотосенсибилизатора, что важно для оценки антибактериальной фотодинамический терапии. Все это позволило выбрать из ряда предложенных образцов наиболее эффективный для антибактериальных задач ФС с наименьшей агрегацией даже при высоких концентрациях, ускорить разработку лекарственной формы и внедрение метода АФДТ в клиническую практику.
- ПубликацияТолько метаданныеDevices for photodynamic studies based on light-emitting diodes(2021) Linkov, K. G.; Borodkin, A. V.; Angelov, I. P.; Meerovich, G. A.; Nekhoroshev, A. V.; Akhlyustina, E. V.; Glechik, D. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович© J-BPE.Light sources based on light-emitting diodes (LED) can be created for almost any wavelength suitable for exciting photosensitizers (PS). This paper presents the main approaches to develop LED-based light sources for well-controlled photodynamic exposure and the results of their implementation. High power density LED light sources were designed and tested for both in vitro and in vivo and photodynamic studies, as well as for irradiation of various pathological foci during photodynamic therapy. © 2021 Journal of Biomedical Photonics & Engineering.
- ПубликацияТолько метаданныеPhotodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers(2021) Meerovich, G. A.; Akhlyustina, E. V.; Denisov, D. S.; Kholina, E. G.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор БорисовичThe aim of this work is the study of antibacterial photodynamic inactivation (APDI) of Escherichia coli (on the example of a model E. coli K12 TG1), the influence of the charge of photosensitizer (PS) molecules on the efficiency of binding with these Gram-negative bacteria. The results obtained confirm that PSs based on polycationic phthalocyanines and synthetic bacteriochlorins provide effective photodynamic inactivation of E. coli, much higher than monocationic methylene blue and electro-neutral bacteriochlorin derivative. The efficiency of APDI, assessed by the intensity of bioluminescence of model E. coli K12 TG1 bacteria, correlates with the charge of PS molecules and the efficiency of electrostatic binding of PS to bacterial cells.