Персона:
Ахлюстина, Екатерина Витальевна

Научные группы

Научная группа

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Ахлюстина

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Екатерина Витальевна

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Теперь показываю 1 - 10 из 30

Только метаданные
Fluorescence diagnostics and photodynamic therapy of grain crops pathogenic fungi
(2020) Bikmukhametova, I. R.; Akhlyustina, E. V.; Pominova, D. V.; Ryabova, A. V.; Grachev, P. V.; Makarov, V. I.; Kartabaeva, B. B.; Ахлюстина, Екатерина Витальевна; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Макаров, Владимир Игоревич
© 2020 IEEE.The studies show the dynamics of photosensitizers accumulation in various grain areas during germination and their photodynamic activity against pathogenic microflora (Fusarium, Bipolaris, Alternaria). Four photosensitizers (methylene blue, Chlorin E6, aluminum phthalocyanine in molecular- and nanoform) were used in the work. The accumulation level of methylene blue and aluminum phthalocyanine in molecular form in infected by Alternaria and Fusarium fungi grains was 4-5 times higher than in control on the 4th day. The possibility of pathogenic microflora inactivation using aluminum phthalocyanine was shown.
Только метаданные
Novel polycationic photosensitizers for antibacterial photodynamic therapy
(2020) Tiganova, I. G.; Lukyanets, E. A.; Makarova, E. A.; Tolordava, E. R.; Meerovich, G. A.; Akhlyustina, E. V.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
© Springer Nature Switzerland AG 2019.Antibacterial photodynamic therapy (APDT) is a promising method of treating local infected foci, in particular, surgical and burn wounds, trophic and diabetic ulcers. Photodynamic inactivation (PDI) is able to effectively destroy bacterial cells without them developing resistance in response to treatment. This work was dedicated to the study of photophysical and antibacterial properties of new photosensitizers (PS) based on polycationic phthalocyanines and synthetic bacteriochlorins for photodynamic inactivation of P. aeruginosa bacteria and their biofilms. Gram-negative bacteria P. aeruginosa are often found in infected wounds, presumably in biofilm state and are characterized by rather low susceptibility to APDT, which is a problem. PS were studied for possible aggregation at various concentrations by means of absorption and fluorescence spectroscopy. The results of studies of the ZnPcChol8, (3-PyHp)4BCBr4 and (3-PyEBr)4BCBr4 in water and serum confirm the assumption of a low degree of their aggregation at high concentrations. Consequently, their photodynamic efficiency is high enabling to use these PS at high concentrations to sensitize pathological foci for APDT. It was shown that all the investigated PS had a high efficiency of photodynamic inactivation of Gram-negative bacteria P. aeruginosa, as well as their biofilms. Tetracationic hydrophilic near-infrared photosensitizer (3-PyEBr)4BCBr4 with reduced molecule size had significantly higher efficacy of photodynamic inactivation of P. aeruginosa biofilms compared with other studied photosensitizers.
Только метаданные
Photodynamic inactivation of Pseudomonas aeruginosa bacterial biofilms using new polycationic photosensitizers
(2019) Tiganova, I. G.; Lukyanets, E. A.; Makarova, E. A.; Tolordava, E. R.; Meerovich, G. A.; Akhlyustina, E. V.; Gonchukov, S. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Гончуков, Сергей Александрович; Лощенов, Виктор Борисович
© 2019 Astro Ltd.The treatment of infected, long-term, non-healing, complicated wounds of the skin and mucosa, trophic ulcers, pressure sores and ulcers of diabetic feet represents a serious problem, especially in the case of resistant and multi-resistant pathogens. Antibacterial photodynamic therapy can be a promising method of local infected foci treatment of such diseases. This work is devoted to the study of the possibility of photodynamic inactivation (PDI) of Pseudomonas aeruginosa bacteria in the form of biofilms with the help of new polycationic photosensitizers (PSs) based on octacationic phthalocyanine and tetra-and octabacteriochlorins (ZnPcChol8, (3-PyBrE)4BCBr4 and (3-PyEPy)4BCBr8). More specifically, this work aims to clarify the role of light irradiation in PDI with the participation of new PSs, which has not been fully studied, especially with respect to the comparison of different PS types.
Только метаданные
Photodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers
(2021) Meerovich, G. A.; Akhlyustina, E. V.; Denisov, D. S.; Kholina, E. G.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
The aim of this work is the study of antibacterial photodynamic inactivation (APDI) of Escherichia coli (on the example of a model E. coli K12 TG1), the influence of the charge of photosensitizer (PS) molecules on the efficiency of binding with these Gram-negative bacteria. The results obtained confirm that PSs based on polycationic phthalocyanines and synthetic bacteriochlorins provide effective photodynamic inactivation of E. coli, much higher than monocationic methylene blue and electro-neutral bacteriochlorin derivative. The efficiency of APDI, assessed by the intensity of bioluminescence of model E. coli K12 TG1 bacteria, correlates with the charge of PS molecules and the efficiency of electrostatic binding of PS to bacterial cells.
Только метаданные
Devices for photodynamic studies based on light-emitting diodes
(2021) Linkov, K. G.; Borodkin, A. V.; Angelov, I. P.; Meerovich, G. A.; Nekhoroshev, A. V.; Akhlyustina, E. V.; Glechik, D. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
© J-BPE.Light sources based on light-emitting diodes (LED) can be created for almost any wavelength suitable for exciting photosensitizers (PS). This paper presents the main approaches to develop LED-based light sources for well-controlled photodynamic exposure and the results of their implementation. High power density LED light sources were designed and tested for both in vitro and in vivo and photodynamic studies, as well as for irradiation of various pathological foci during photodynamic therapy. © 2021 Journal of Biomedical Photonics & Engineering.
Только метаданные
Antitumor activity of photodynamic therapy with tetracationic derivative of synthetic bacteriochlorin in spheroid culture of liver and colon cancer cells
(2022) Karshieva, S. S.; Glinskaya, E. G.; Dalina, A. A.; Akhlyustina, E. V.; Mironov, V. A.; Meerovich, G. A.; Kogan, E. A.; Koudan, E. V.; Ахлюстина, Екатерина Витальевна; Миронов, Владимир Александрович; Меерович, Геннадий Александрович; Кудан, Елизавета Валерьевна
Efficient screening of photosensitizers (PS) as well as studying their photodynamic activity, especially PS excited in the near-infrared region, require informative in vitro models to adequately reflect the architecture, thickness, and intercellular interactions in tumors. In our study, we used spheroids formed from human colon cancer HCT-116 cells and liver cancer Huh7 cells to assess the phototoxicity of a new PS based on tetracationic derivative of synthetic bacteriochlorin (BC4). We optimized conditions for the irradiation regime based on the kinetics of BC4 accumulation in spheroids and kinetics of spheroid growth. Although PS accumulated more efficiently in HCT-116 cells, characterized by more aggressive growth and high proliferative potential, they were less susceptible to the photodynamic therapy (PDT) compared to the slower growing Huh7 cells. We also showed that 3D models of spheroids were less sensitive to BC4 than conventional 2D cultures with relatively identical kinetics of drug accumulation. Our findings suggest that BC4 is a perspective agent for photodynamic therapy against cancer cells. Copyright © 2022 Elsevier B.V. All rights reserved.
Только метаданные
On the mechanisms of photodynamic action of photosensitizers based on polycationic derivatives of synthetic bacteriochlorin against human lung cancer cells A549 (in vitro study)
(2022) Kogan, E. A.; Meerovich, G. A.; Karshieva, S. S.; Makarova, E. A.; Romanishkin, I. D.; Akhlyustina, E. V.; Meerovich, I. G.; Zharkov, N. V.; Demura, T. A.; Chen, Z. -L.; Koudan, E. V.; Angelov, I. P.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна
Background: One of the tasks of anticancer photodynamic therapy is increasing the efficacy of treatment of cancer nodes with large (clinically relevant) sizes using near-infrared photosensitizers (PS). We study the photodynamic action against A549 human lung cancer cells using PS based on polycationic derivatives of synthetic bacteriochlorin. Methods: The efficacy and mechanisms of the photodynamic action of PS based on polycationic derivatives of synthetic bacteriochlorin against A549 lung cancer cells were studied in vitro using immunocytochemical and morphological methods. Results: It was found that PS based on tetracationic and octacationic derivatives of synthetic bacteriochlorin induce necrosis, apoptosis, decreasing of proliferative and mitotic activity, as well as reducing the number of ALDH1-positive cancer cells with signs of stem cells in A549 human lung cancer cell culture. The IC50 values (concentration of a PS that reduces cells survival by 50%) were about 0.69 μM for tetracationic PS and 0.57 μM for octacationic PS under irradiation at 30 J/cm2 while in the “dark” control they were higher than 100 μM for both PSs. Conclusions: Photosensitizers based on polycationic derivatives of synthetic bacteriochlorin have high phototoxicity against A549 cancer cells caused by the induction of necrosis and apoptosis of cancer cells, including cells with signs of stemness, and a sharp decrease of mitotic and proliferative activity. © 2022
Только метаданные
Спектрально-оптические свойства катионных фотосенсибилизаторов на основе производных бактериохлорина для антибактериальной фототераностики в ближнем ИК-диапазоне
(2021) Ахлюстина, Е. В.; Ахлюстина, Екатерина Витальевна; Лощенов Виктор Борисович
На сегодняшний день разработка и исследование новых катионных фотосенсибилизаторов, таких как катионные бактериохлорины, являются актульными задачами, поскольку они показали более высокую чем нейтральные и анионные фотосенсибилизаторы в отношении инактивации бактерий. В частности, необходимо подобрать наиболее эффективные фотосенсибилизаторы из ряда синтезированных производных катионных бактерихлоринов для задач антибактериальной фотодинамический терапии. Для решения этой задачи были исследованы их фотофизические свойства, такие как поглощение, флуоресценция, фотобличинг(фотодеградация), времена жизни флуоресценции, агрегация. Другой важной задачей является эквивалентное облучение образцов для микробиологических исследований. В работе была проведена оценка доли поглощенной энергии в тонких слоях при работах in vitro в зависимости от источника облучения и характеристик фотосенсибилизатора, что важно для оценки антибактериальной фотодинамический терапии. Все это позволило выбрать из ряда предложенных образцов наиболее эффективный для антибактериальных задач ФС с наименьшей агрегацией даже при высоких концентрациях, ускорить разработку лекарственной формы и внедрение метода АФДТ в клиническую практику.
Только метаданные
On the possibility of photodynamic inactivation of tracheobronchial tree pathogenic microbiota using methylene blue (in vitro study)
(2022) Tiganova, I. G.; Zulufova, I. D.; Ovchinnikov, R. S.; Solovyev, A. I.; Meerovich, G. A.; Akhlyustina, E. V.; Kozlikina, E. I.; Nekhoroshev, A. V.; Glechik, D. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
© 2022Background: The treatment of patients after mechanical ventilation of lungs suffering from a multi-species infection of the tracheobronchial tree can be complicated. The situation is aggravated in patients with post-intubation tracheal stenosis, where infection plays a leading pathogenetic role in damage to the tracheal wall. As a result of such a pathological process, cicatricial stenosis of the trachea of purulent-inflammatory infectious genesis or infected tracheal stenosis (ITS) may occur. Methods: In this work, we studied the possibility of photodynamic inactivation of pathogenic microbiota typical for patients with ITS using methylene blue (MB) as a photosensitizer. Results: 13 clinical isolates of 8 species of bacteria from 9 patients were susceptible to photodynamic inactivation with MB. 30 μM of MB at a light irradiation dose of 25 J/cm2 and incubation with MB for 15 min allows to completely inactivate bacteria found in the tracheobronchial secretions of patients with ITS. Conclusions: MB retains its optico-physical properties in the range of 3–30 μM and provides effective inactivation of isolated Gram-positive and Gram-negative bacteria, including multi- and pan-resistant to antibiotics.
Только метаданные
Synthesis of Gold Nanoparticles from Aqueous Solutions of Hydrochloroauric Acid under Multipulse Femtosecond Irradiation
(2022) Ashikkalieva, K. K.; Kononenko, V. V.; Vasil'ev, A. L.; Gololobov, V. M.; Akhlyustina, E. V.; Ахлюстина, Екатерина Витальевна
© 2022, Allerton Press, Inc.Abstract: The main modes of synthesis of gold nanoparticles in aqueous solutions of hydrochloroauric acid under multipulse (3 × 106) femtosecond laser irradiation have been studied. UV–Vis spectroscopy, dynamic light scattering, and transmission electron microscopy (TEM) showed that there are two types of nanoparticles dominating in the investigated range of laser-pulse energies W = 1.6–200 µJ: ultrasmall (~1–5 nm) and plasmonic (~5–50 nm) nanoparticles. The particle size is found to be determined by two threshold pulse energies. The first corresponds to the development of avalanche water ionization, which initiates reduction of [AuCl4]– ions to neutral atoms, formation of nuclei for nanoparticles, and their subsequent growth. The second threshold is determined by the avalanche-plasma heating, which leads to an explosive rise in the liquid temperature with overheating, melting, and fragmentation of previously formed gold nanoparticles.