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Савельева, Татьяна Александровна

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Организационные подразделения

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Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Савельева

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Татьяна Александровна

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Теперь показываю 1 - 10 из 37

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Differentiation of glioblastoma tissues using spontaneous Raman scattering with dimensionality reduction and data classification
(2022) Romanishkin, I.; Savelieva, T.; Orlov, A.; Loschenov, V.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
The neurosurgery of intracranial tumors is often complicated by the difficulty of distinguishing tumor center, infiltration area, and normal tissue. The current standard for intraoperative navigation is fluorescent diagnostics with a fluorescent agent. This approach can be further enhanced by measuring the Raman spectrum of the tissue, which would provide additional information on its composition even in the absence of fluorescence. However, for the Raman spectra to be immediately helpful for a neurosurgeon, they must be additionally processed. In this work, we analyzed the Raman spectra of human brain glioblastoma multiforme tissue samples obtained during the surgery and investigated several approaches to dimensionality reduction and data classificatin to distinguish different types of tissues. In our study two approaches to Raman spectra dimensionality reduction were approbated and as a result we formulated new technique combining both of them: feature filtering based on the selection of those shifts which correspond to the biochemical components providing the statistically significant differences between groups of examined tissues (center of glioblastoma multiforme, tissues from infiltration area and normally appeared white matter) and principal component analysis. We applied the support vector machine to classify tissues after dimensionality reduction of registered Raman spectra. The accuracy of the classification of malignant tissues (tumor edge and center) and normal ones using the principal component analysis alone was 83% with sensitivity of 96% and specificity of 44%. With a combined technique of dimensionality reduction we obtained 83% accuracy with 77% sensitivity and 92% specificity of tumor tissues classification.
Только метаданные
Combined spectroscopic and video fluorescent instrument for intraoperative navigation when removing a glial tumor
(2020) Loshchenov, M. V.; Borodkin, A. V.; Linkov, K. G.; Kosyrkova, A. V.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2020 SPIE.The new approach to intraoperative navigation during glial brain tumors removal is presented. A combined method is proposed for simultaneous spectroscopic and video fluorescence analysis of the state of tissues in the destruction zone using the applied part performed in the form of a neurosurgical aspirator cannula. In the walls of the applied part there are tubular channels into which lighting and receiving optical fibers are integrated. At the end of the cannula, the channels for optical fibers are arranged so as to perform spectroscopic analysis in contact with the surface of the biological tissue, as well as video fluorescence analysis at the working distance to the surface of the tissue. The joint use of fiber-optic systems for recording the video stream and spectral dependences allows real-time assessment of the degree of pathological tissue changes in the field of view of the video system, which are also located in the aspiration zone, with the simultaneous quantification of diagnostically significant spectroscopic criteria. System testing was carried out on samples of human intracranial tumors obtained during neurosurgical operations. During the removal of a tumor from different sites (tumor center, perifocal area), the degree of in vivo fluorescence signal from the tumor site was determined intraoperatively using a Zeiss Opmi Pentero intraoperative microscope in Blue 400 mode. From the selected area of the tumor, biopsy material was taken (presumably homogeneous in its properties) with subsequent measurement of spectra and combined images using the developed device. A high correlation was shown between the level of the fluorescence signal recorded spectroscopically and the brightness of the fluorescence image in the endoscopic channel of the device. The level of the fluorescent signal showed a high correlation with the degree of malignancy of tissues according to the results of pathomorphological examination.
Только метаданные
Evaluating the dynamics of brain tissue oxygenation using near-infrared spectroscopy on various experimental models
(2019) Kustov, D. M.; Sharova, A. S.; Makarov, V. I.; Borodkin, A. V.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2019 Astro Ltd.In this paper we consider a method for researching the dynamics of blood flow in the cerebral cortex, on an optical phantom that reproduces the parameters of real human and mice brain structures, through the use of near and infrared ranges of laser radiation. For the investigation of real tissue we chose a laboratory mouse brain in vivo. An algorithm for non-invasive diagnostics of the degree of oxygenation was identified and optimal parameters of installation components were selected for taking information about hemodynamic indicators. Output was verified by the reference method for assessing oxygenation by degree of absorption of hemoglobin in the visible range, which indicates that data have a high correlation with classical methods. With further development, this algorithm can be used in various areas of research and diagnostics.
Только метаданные
STUdy OF ENERGy TRANSFER pROCESSES BETWEEN RARE EARTh IONS ANd phOTOSENSITIZER mOLECULES FOR phOTOdyNAmIC ThERApy WITh IR-EXCITATION ИССЛЕдОвАНИЕ пРОЦЕССОв пЕРЕдАчИ ЭНЕРГИИ мЕЖдУ РЕдКОЗЕмЕЛЬНЫмИ ИОНАмИ И мОЛЕКУЛАмИ фОТОСЕНСИБИЛИЗАТОРОв дЛя ЗАдАч фОТОдИНАмИчЕСКОи ТЕРАпИИ С вОЗБУЖдЕНИЕм в ИК-дИАпАЗОНЕ
(2021) Proydakova, V. Y.; Romanishkin, I. D.; Kuznetsov, S. V.; Lukyanets, E. A.; Pominova, D. V.; Bogatova, A. S.; Akhlyustina, E. V.; Saveleva, T. A.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Ахлюстина, Екатерина Витальевна; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2021 Russian Photodynamic Association. All rights reserved.Today, photodynamic therapy is one of the most promising minimally invasive methods of treatment of various diseases, including cancer. The main limitation of this method is the insufficient penetration into the tissue of laser radiation used to activate photosensitizer molecules,which makes it difficult to carry out therapy in the treatment of large or deep-seated tumors. In this regard, there is a great interest in thedevelopment of new strategies for photodynamic therapy using infrared radiation for excitation, the wavelengths of which fall into the “transparencywindow” of biological tissues. In this work, it was proposed to use upconversion NaGdF4:Yb:Er nanoparticles (UCNP), which absorbinfrared excitation and serve as a donor that transfers energy to the photosensitizer. Photosens and phthalosens were chosen as the mostpromising photosensitizers for the study. The aim of this work was to study the energy transfer processes between upconversion nanoparticlesdoped with rare-earth ions and photosensitizer molecules. in order to excite photosensitizers with IR radiation and carry out photodynamictherapy of deep-seated neoplasms. Using spectroscopic and time-resolved methods, it has been demonstrated that there is an efficientenergy transfer between upconversion particles and photosensitizers phthalosens and photosens. The calculated efficiency of energy transferby the Foerster mechanism was 41% for the UCNP + photosens system and 69% for the UCNP + phthalosens system. It has been experimentallyand theoretically proved that there is a binding of photosensitizer molecules with UCNP by means of surfactants, leading to a reductionin the distance between them, due to which effective nonradiative energy transfer is realized. The generation of singlet oxygen by the phthalosensphotosensitizer upon excitation by means of energy transfer from UCNP, excited at 980 nm wavelength of, has been demonstrated.
Только метаданные
The optical estimation of glioma cell composition using fluorescence lifetime imaging
(2020) Maklygina, Y. S.; Romanishkin, I. D.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
Gliomas are one of the most common brain tumors occurring in children and adults. Gliomas are primary, diffusely infiltrating brain tumors. There are few effective therapies for these type of cancer, and patients with malignant glioma fare poorly, even after aggressive surgery, chemo- and radiotherapy.
Только метаданные
Investigation of a combined absorption-fluorescence-Raman spectroscopy approach to brain tumor tissue differentiation ex vivo
(2022) Romanishkin, I. D.; Savelieva, T. A.; Poletaeva, I. Yu.; Shugai, S. V.; Goryaynov, S. A.; Golbin, D. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
In this work, we used a combined absorption-fluorescence-Raman approach to the optical investigation of brain tissue. The proposed approach allowed taking into account the blood fill, oxygenation, accumulation of fluorescent biomarker, and Raman signal to estimate a degree of malignancy of the tissue. © 2022 IEEE.
Только метаданные
Method for rapid intraoperative analysis of the optical properties of multilayered walls of hollow organs
(2024) Savelieva, T. A.; Krivetskaya, A. A.; Levkin, V. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Кривецкая, Анна Александровна; Лощенов, Виктор Борисович
Только метаданные
Evaluation of tissue blood supply during esophagectomy using fluorescent diagnostics and diffuse scattering spectroscopy in visible region
(2024) Krivetskaya, A. A.; Kustov, D. M.; Savelieva, T. A.; Loschenov, V. B.; Кривецкая, Анна Александровна; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
The success of the surgical treatment of a tumor or obstruction of the esophagus with subsequent anastomosis application depends on the level of blood supply to the stitched tissues. Intraoperative assessment of blood flow is widely used in medicine and can be used as a diagnostic method that affects the outcome of surgery and reduces the frequency of postoperative complications for the patient. In this work, the assessment of blood supply during esophageal resection operations was carried out using two techniques sequentially: fluorescent diagnostics with indocyanine green and measurement of hemoglobin oxygen saturation by diffuse scattering spectroscopy in the visible wavelength range. The first method was used to assess the integrity of the vascular network structure in the area of anastomosis and blood flow through the sutured tissues, the second one вЂ“ for local assessment of hemoglobin oxygen saturation in the investigated area. Conducted clinical study involved the participation of nine patients with malignant neoplasms (six cases) or esophageal obstruction (three cases). The presence of postoperative complications was compared with the measurement results. Anastomosis failure was observed in only one patient. According to the results of the study, with the use of the investigated method of assessing blood supply, there is a tendency towards a decrease in the frequency of anastomosis leaks (11.1 % compared with 21.4 %). Therefore, fluorescent diagnostics with indocyanine green and measurement of hemoglobin oxygen saturation using diffuse scattering spectroscopy were affirmed as methods that allow increasing the safety of surgical procedures by assessing the risk of postoperative complications, including anastomosis failures.
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The Spectroscopic Study of Indocyanine Green J-Aggregate Stability in Human Blood and Plasma
(2022) Farrakhova, D. S.; Romanishkin, I. D.; Yakovlev, D. V.; Maklygina, Y. S.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович
© 2022, Allerton Press, Inc.Abstract: Spectroscopic approaches are very good to noninvasively determine the most significant indicators of the tissue state. Indocyanine green (ICG) is a well-known fluorescent dye approved for clinical applications, which has a short circulation time in the vascular system and low photostability. At high temperatures the molecular solution of the photosensitizer self-assembles into a stable J-aggregate form of ICG nanoparticles (ICG NPs) with the absorption peak in the near-infrared range. Investigation of ICG NP stability in human blood and plasma using a fiber-spectroscopic system demonstrates no difference in absorption properties and different dependence of the integrated fluorescence ratio between ICG monomers and J-aggregates in blood and plasma. Transition of ICG NP aggregates to the monomeric form in human blood plasma results in a higher circulation time of the fluorescent dye in the vascular system. High stability of aggregates and a low elimination rate may increase efficiency of fluorescent diagnostics of near-tumor tissues.
Только метаданные
Multimodal method of tissue differentiation in neurooncology using Raman spectroscopy, fluorescence and diffuse reflectance spectroscopy
(2022) Romanishkin, I. D.; Ospanov, A.; Savelyeva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович; Оспанов, Ануар