Персона:
Залыгин, Антон Владленович

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

Фамилия

Залыгин

Имя

Антон Владленович

Полная страница элемента

Результаты поиска

Теперь показываю 1 - 10 из 11

Открытый доступ
Исследование локализации микровезикул в клетке методом сканирующей флуоресцентной конфокальной микроспетроскопии
(2024) Белицкая, Е. Д.; Сливка, Е. В.; Олейников, В. А.; Залыгин, А. В.; Олейников, Владимир Александрович; Залыгин, Антон Владленович
В работе представлены первые результаты серии экспериментов по изучению механизма связывания микровезикул с адресной клеткой с помощью метода сканирующей флуоресцентной конфокальной микроскопии. Разработан новый подход для визуализации проникновения микровезикул в клетку.
Только метаданные
Loading efficiency of doxorubicin into the micelle-like structures formed by function-spacer-lipid constructs self-assembly depends on constructs' functional part
(2021) Tarasova, A. R.; Vaskan, I. S.; Troitskaya, P. S.; Bovin, N. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Supramolecular self-assemble systems based on neoglycolipids: Galili-Ad-CMG2-Ad-DOPE, A(type2)-Ad-CMG2-Ad-DOPE are studied here and compared with the well-studied Biotin-CMG2-Ad-DOPE, as well as with their combinations with NH2-CMG2-Ad-DOPE. They are function-spacer-lipid constructs with unique structure that allows them to form micelle-like supramers and be stable, what makes them a potential drug nanocarriers. The structural properties of the obtained supramolecular systems are studied depending on their functional part, and the loading efficiency of doxorubicin into the supramers is determined to reveal the influence of the functional part. The resulting supramers were separated from the unbound molecules by dialysis, the nanoparticles morphology were studied by atomic force microscopy, and the loading efficiency was calculated based on spectrophotometry data. The encapsulation of doxorubicin was confirmed based on changes in the size and shape of the supramers, as well as a decrease in the ratio of unbound molecules. According to the loading efficiency calculations, it was estimated that supramers formed by A(type2)-Ad-CMG2-Ad-DOPE are the most efficient nanocarriers with loading efficiency of 82 %. Supramers formed by NH2-CMG2-Ad-DOPE (no functional part) showed 1.5 times less efficiency. Finally, the least efficient carriers are supramers formed by Biotin-CMG2-Ad-DOPE (14%).
Только метаданные
Small-angle X-ray (SAXS) and Raman spectroscopy studies of biot-CMG(2)-DOPE quasicrystalline phases
(2021) Maslennikov, A. M.; Shtykova, E. V.; Bovin, N. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Neoglycolipids due to their amphiphilic properties exhibit self-assembly in aqueous phases. In high concentrations the liquid crystalline or gel phases may form. So-called soft-material are a subject of interest of many scientists especially as biosensors and wound healing materials. In this study we examine the structure of a quasicrystalline phase of biot-CMG(2)DOPE obtained at the concentration of 150 mg/ml (13wt.%) in PBS. The structural data such as interplanar spacing, order parameter and long-range order were obtained by SAXS, while the changes in chemical structure were studied by Raman spectroscopy. It was also in our interest to examine a correlation between the ionic strength and the self-assembly, so we also studied a similar quasicrystalline phase of the same compound but in a buffer containing CaCl2 at the concentration of 4wt.%. According to SAXS data, FSL-biotin construct formed a complex ordered phase consisting of overlapping latices of different kind. The addition of CaCl2 into PBS resulted in obtaining a more structured system demonstrating cubic-like crystal lattice. Change in peak intensities on Raman spectrums of -C-H- and -C-C- bonds vibrations explained the change in phase properties.
Только метаданные
Comparative analysis of SERS-active colloidal silver solutions of various type and prospects of their applications
(2021) Altunina, A. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Raman spectroscopy is a promising method for optical vibrational spectroscopy. Nowadays, Raman spectroscopy finds many applications, in particular, in biological and medical diagnostics. However, the Raman scattering can be enhanced using the Surface-enhanced Raman scattering method. Colloidal solutions of noble metals are used as SERS-active systems. In this work, the enhancing factors were estimated for colloidal silver solutions of three different types (citrate, borohydride, chloride) with two substances (phenylalanine, cytochrome C). Phenylalanine is a widely used model substance for Raman and Surface-enhanced Raman spectroscopy. Cytochrome C is one of the most researched proteins. It involves in the electron transport chain of the mitochondrial inner membrane and provides cellular respiration. Borohydride, citrate and chloride sols with phenylalanine gave an enhancement about 50, 200 and 30 times, respectively, and with cytochrome C about 30, 160 and 20, respectively. A comparative analysis of active and inactive sols by SERS and absorption spectroscopy was also performed. The absorption spectra of active sols have characteristic maxima in the region of 400 nm. Both the SERS method of model substances and absorption spectroscopy can be used to assess the enhancing properties.
Только метаданные
Hyperglycemia exacerbates ischemic stroke not through increased generation of hydrogen peroxide
(2023) Kotova, D. A.; Ivanova, A. D.; Pochechuev, M. S.; Kelmanson, I. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
Только метаданные
Study of Localization of Microvesicles in a Cell by Scanning Fluorescence Confocal Microspectroscopy
(2023) Belitskaya, E. D.; Slivka, E. V.; Oleinikov, V. A.; Zalygin, A. V.; Олейников, Владимир Александрович; Залыгин, Антон Владленович
Только метаданные
Assessment of core-shell nanoparticles surface structure heterogeneity by SAXS contrast variation and ab initio modeling
(2023) Vaskan, I. S.; Prikhodko, A. T.; Petoukhov, M. V.; Oleinikov, V. A.; Zalygin, A. V.; Олейников, Владимир Александрович; Залыгин, Антон Владленович
Только метаданные
Effect of ligand and shell densities on the surface structure of core-shell nanoparticles self-assembled from function-spacer-lipid constructs
(2023) Vaskan, I.; Dimitreva, V.; Petoukhov, M.; Oleinikov, V.; Zalygin, A.; Олейников, Владимир Александрович; Залыгин, Антон Владленович; Димитрева, Вероника Алексеевна
Только метаданные
Study of Self-Assembly of Cyclodextrin Conjugates with Phospholipid by Molecular Dynamics Method
(2023) Dimitreva, V. A.; Vaskan ,I. S.; Oleinikov, V. A.; Zalygin, A. V.; Олейников, Владимир Александрович; Залыгин, Антон Владленович; Димитрева, Вероника Алексеевна
Только метаданные
Radiopharmaceuticals for Non-Glucose-Specific Oncology (PET and SPECT) (A Review)
(2023) Belitskaya, E. D.; Dimitreva, V. A.; Kozlov, A. N.; Oleinikov, V. A.; Zalygin, A. V.; Козлов, Андрей Николаевич; Олейников, Владимир Александрович; Залыгин, Антон Владленович; Димитрева, Вероника Алексеевна