Персона:
Тимошенко, Виктор Юрьевич

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Тимошенко

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Виктор Юрьевич

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Только метаданные
Bi-Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics
(2019) Rogov, A.; Ryabchikov, Y. V.; Geloen, A.; Tishchenko, I.; Kharin, A. Y.; Lysenko, V.; Zavestovskaya, I. N.; Kabashin, A. V.; Timoshenko, V. Y.; Завестовская, Ирина Николаевна; Кабашин, Андрей Викторович; Тимошенко, Виктор Юрьевич
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Presenting a safe alternative to conventional compound quantum dots and other functional nanostructures, nanosilicon can offer a series of breakthrough hyperthermia-based therapies under near-infrared, radiofrequency, ultrasound, etc., excitation, but the size range to sensitize these therapies is typically too large (>10 nm) to enable efficient imaging functionality based on photoluminescence properties of quantum-confined excitonic states. Here, it is shown that large Si nanoparticles (NPs) are capable of providing two-photon excited luminescence (TPEL) and second harmonic generation (SHG) responses, much exceeding that of smaller Si NPs, which promises their use as probes for bi-modal nonlinear optical bioimaging. It is finally demonstrated that the combination of TPEL and SHG channels makes possible efficient tracing of both separated Si NPs and their aggregations in different cell compartments, while the resolution of such an approach is enough to obtain 3D images. The obtained bi-modal contrast provides lacking imaging functionality for large Si NPs and promises the development of novel cancer theranostic modalities on their basis.
Только метаданные
Reflectance Modification in Nanostructured Silicon Layers with Gradient Porosity
(2019) Mussabek, G. K.; Yermukhamed, D.; Suleimenova, Z. A.; Assilbayeva, R. B.; Zavestovskaya, I. N.; Timoshenko, V. Y.; Завестовская, Ирина Николаевна; Тимошенко, Виктор Юрьевич
A significant change in effective reflectance spectra of nanostructured porous silicon layers grown with different times of metal-assisted chemical etching is detected. The low reflectances at the level of 5-10% measured in the spectral range of 200-400 nm are explained by strong elastic scattering of light in combination with absorption in silicon nanostructures, while a reflectance increase in the range of 500-1800 nm, which is visually detected as a "white" layer appearance is associated with Mie scattering in silicon nanostructures with gradient porosity under conditions of weak optical absorption. The results obtained are discussed from the viewpoint of potential applications of "black" and "white" nanocrystalline silicon in photonics and sensorics.
Только метаданные
Radiofrequency Heating of Nanoparticles for Biomedical Applications
(2021) Grigoriev, A. A.; Grigoryeva, M. S.; Kargina, Y. V.; Kharin, A. Y.; Zavestovskaya, I. N.; Kanavin, A. P.; Timoshenko, V. Y.; Григорьев, Андрей Андреевич; Григорьева, Мария Сергеевна; Завестовская, Ирина Николаевна; Канавин, Андрей Павлович; Тимошенко, Виктор Юрьевич
© 2021, Allerton Press, Inc.Abstract: The heat release during electromagnetic high-frequency (HF) heating of solid-state nanoparticle (NP) suspensions in an electrolyte with physiological solution conductivity is studied. It is shown that heating of a colloidal NP solution in an electrolyte should be calculated taken into account the contribution of an electric double layer formed near the NP surface, and the heating efficiency is controlled by the NP conductivity and the ratio of the frequency of radio-frequency radiation and the electrolyte conductivity. The optimal conductivity of silicon-based NPs for hyperthermia is determined as a function of the HF radiation frequency.
Только метаданные
Effect of electrolyte conductivity and local electric field inhomogeneity on heating of an aqueous suspension of solid-state nanoparticles
(2021) Grigoriev, A. A.; Zavestovskaya, I. N.; Kharin, A. Yu.; Kanavin, A. P.; Kargina, Yu. V.; Timoshenko, V. Yu.; Тимошенко, Виктор Юрьевич
© 2021 Institute of Physics Publishing. All rights reserved.This work theoretically investigates the process of heat release in an electrolyte near a solid-state nanoparticle when exposed to an external radio-frequency electromagnetic field. The effect on the heat release of the effect of a change in the electrical conductivity of the electrolyte near the nanoparticle due to the redistribution of the ion concentration and the inhomogeneity of the electric field caused by the difference in the dielectric constants of the electrolyte and the nanoparticle is taken into account. The proposed model can be useful for choosing the optimal parameters of nanoparticles and radio frequency radiation when used in biomedicine for tumor hyperthermia.
Только метаданные
Effect of silicon target porosity on laser ablation threshold: molecular dynamics simulation
(2021) Kharin, A. Y.; Grigoryeva, M. S.; Zavestovskaya, I. N.; Timoshenko, V. Y.; Григорьева, Мария Сергеевна; Завестовская, Ирина Николаевна; Тимошенко, Виктор Юрьевич
Ablation of a porous silicon target under irradiation with ultrashort laser pulse is simulated by means of the molecular dynamics approach. The number of ablated atoms is calculated for targets with different porosity under irradiation with wavelengths in ultraviolet (UV) and visible spectral ranges, which correspond to stronger and weaker absorption coefficient, respectively. An increase of the porosity to 80% leads to 1.5-3 times decrease of the ablation threshold compared to the bulk silicon, while a decrease of pores size from 2.5 to 1.2 nm leads to the stronger ablation threshold drop and the effect is stronger for the UV irradiation. The results are useful for laser processing of silicon-based targets and nanofabrication.
Только метаданные
Measurement of silicon nanoparticles temperature by raman spectroscopy
(2021) Alykova, A. F.; Grigoryeva, M. S.; Zavestovskaya, I. N.; Timoshenko, V. Yu.; Григорьева, Мария Сергеевна; Завестовская, Ирина Николаевна; Тимошенко, Виктор Юрьевич
© 2021 Journal of Biomedical Photonics & Engineering.The temperature of silicon nanoparticles under laser photo-induced heating is determined from the ratio of the intensities of the Stokes and anti-Stokes components of the Raman scattering. The obtained results of the dependence of nanoparticles temperature on the laser radiation intensity and the temperature dependence of the Raman line position may be used to determine the optimal regimes of photo-hyperthermia enhanced by silicon nanoparticles for cancer therapy.
Открытый доступ
Localized infrared radiation-induced hyperthermia sensitized by laser-ablated silicon nanoparticles for phototherapy applications
(2020) Oleshchenko, V. A.; Karpukhina, O. V.; Bezotosnyi, V. V.; Kharin, A. Y.; Alykova, A. F.; Karpov, N. V.; Popov, A. A.; Klimentov, S. M.; Zavestovskaya, I. N.; Kabashin, A. V.; Timoshenko, V. Y.; Попов, Антон Александрович; Климентов, Сергей Михайлович; Завестовская, Ирина Николаевна; Кабашин, Андрей Викторович; Тимошенко, Виктор Юрьевич
© 2020 Elsevier B.V.Silicon (Si) nanoparticles (NPs) synthesized by methods of laser ablation in water are explored as sensitizers of photothermal therapy under a laser excitation in the window of relative tissue transparency. Based on theoretical calculations and experimental data, it is shown that the NPs can be heated up to temperatures above 42–50 °C by laser diode irradiation at 808 nm in continuous wave (CW) and quasi-continuous wave (QCW) regimes. Profiting from the laser-induced heating, a high efficiency Si-NPs as sensitizers of the hyperthermia of cells in Paramecium Caudatum model is demonstrated. The QCW regime is found to be more efficient, leading to complete cell destruction even under relatively mild laser irradiation conditions. The obtained data evidence a great potential in using laser-ablated Si-NPs as sensitizers of photohyperthermia in antibacterial or cancer therapy applications.
Открытый доступ
Nuclear nanomedicine using Si nanoparticles as safe and effective carriers of 188 Re radionuclide for cancer therapy
(2019) Tischenko, V. K.; Mikhailovskaya, A. A.; Popov, A. A.; Tselikov, G.; Petriev, V. M.; Deyev, S. M.; Timoshenko, V. Y.; Prasad, P. N.; Zavestovskaya, I. N.; Kabashin, A. V.; Деев, Сергей Михайлович; Тимошенко, Виктор Юрьевич; Завестовская, Ирина Николаевна; Кабашин, Андрей Викторович
© 2019, The Author(s). Nuclear nanomedicine, with its targeting ability and heavily loading capacity, along with its enhanced retention to avoid rapid clearance as faced with molecular radiopharmaceuticals, provides unique opportunities to treat tumors and metastasis. Despite these promises, this field has seen limited activities, primarily because of a lack of suitable nanocarriers, which are safe, excretable and have favorable pharmacokinetics to efficiently deliver and retain radionuclides in a tumor. Here, we introduce biodegradable laser-synthesized Si nanoparticles having round shape, controllable low-dispersion size, and being free of any toxic impurities, as highly suitable carriers of therapeutic 188 Re radionuclide. The conjugation of the polyethylene glycol-coated Si nanoparticles with radioactive 188 Re takes merely 1 hour, compared to its half-life of 17 hours. When intravenously administered in a Wistar rat model, the conjugates demonstrate free circulation in the blood stream to reach all organs and target tumors, which is radically in contrast with that of the 188 Re salt that mostly accumulates in the thyroid gland. We also show that the nanoparticles ensure excellent retention of 188 Re in tumor, not possible with the salt, which enables one to maximize the therapeutic effect, as well as exhibit a complete time-delayed conjugate bioelimination. Finally, our tests on rat survival demonstrate excellent therapeutic effect (72% survival compared to 0% of the control group). Combined with a series of imaging and therapeutic functionalities based on unique intrinsic properties of Si nanoparticles, the proposed biodegradable complex promises a major advancement in nuclear nanomedicine.
Открытый доступ
Morphophysiological changes in the intact surface of rat skin under the application of silicon and gold nanoparticles
(2019) Kondratenko, E. I.; Lomteva, N. A.; Kasimova, S. K.; Yakovenkova, L. A.; Timoshenko, V. Yu.; Alykova, A. F.; Zavestovskaya, I. N.; Тимошенко, Виктор Юрьевич; Завестовская, Ирина Николаевна
© Published under licence by IOP Publishing Ltd.The effect of a course application of suspension of porous silicon and gold nanoparticles on the skin of female rats on the level of free radical oxidation and catalase activity in the skin homogenate was studied. MesoPSi samples were formed using the standard method of electrochemical etching of p-type c-Si wafers with a surface orientation of (100) and a specific resistance of 1-5 mOm x cm in a solution of hydrofluoric acid and ethanol (HF (50%): C2H5OH = 1: 1) at an etching current density of 60 mA / cm2 for 1 hour (45 minutes-hour). After this, mesoPSi films were separated from the silicon substrate by a short-term increase in the current density to 600 mA / cm2. Aqueous suspensions of porous silicon nanoparticles with sizes of the order of 100 nm were obtained by mechanical grinding of mesoporous silicon films in water. Aqueous suspensions of gold nanoparticles with an average size of 30-50 nm were obtained by laser ablation of gold targets in deionized water. A suspension of silicon and gold nanoparticles in physiological saline at a concentration of 0.2 mg / ml was applied once a day in the form of a thin layer on a shaved area of the skin of animals (interscapular region) and left to dry completely. Applications were carried out for 10 days. The studied silicon and gold nanoparticles did not change the initial content of malondialdehyde and catalase activity in the skin tissue but contributed to the activation of the rate of spontaneous lipid peroxidation.
Открытый доступ
Effect of long-wavelength coherent radiation on a biological object (unicellular organism Paramecium Caudatum) in presence of silicon nanoparticles
(2019) Oleshenko, V. A.; Karpukhina, O. V.; Alykova, O. M.; Bezotosniy, V. V.; Alykova, A. F.; Karpov, N. V.; Klimentov, S. M.; Timoshenko, V. Y.; Zavestovskaya, I. N.; Климентов, Сергей Михайлович; Тимошенко, Виктор Юрьевич; Завестовская, Ирина Николаевна
© 2019 Published under licence by IOP Publishing Ltd. We report an effect of continuous and pulsed near infrared (808 nm) laser radiation on cells (Paramecium Caudatum) incubated with silicon (Si) nanoparticles (NPs), which were obtained by laser ablation of crystalline Si wafer in water. The data obtained indicate the possibility of enhancing the destructive effect on cells by using pulsed laser irradiation and Si NPs.