Персона: Захаркив, Анастасия Юрьевна
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Анастасия Юрьевна
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- ПубликацияТолько метаданныеInteraction of SiFe Nanoparticles with Epithelial and Lymphoid Cells(2020) Sharonova, N. V.; Svirshchevskaya, E. V.; Popov, A. A.; Karpov, N. V.; Tikhonovsky, G. V.; Zakharkiv, A. Y.; Timoshenko, V. Y.; Klimentov, S. M.; Oleinikov, V. A.; Попов, Антон Александрович; Тихоновский, Глеб Валерьевич; Захаркив, Анастасия Юрьевна; Тимошенко, Виктор Юрьевич; Климентов, Сергей Михайлович; Олейников, Владимир АлександровичSilicon and silicon-based nanoparticles (SiNP) attract scientific attention due to the biocompatibility and assimilation of silicon by body tissues. Iron-doped SiNP (SiFeNP) allow the use of ferromagnetic properties of iron for NP detection and the possibility of therapeutic application of SiFeNP. The purpose of this work was to analyze the interaction of SiFeNP with epithelial cells (EC) COLO357 and SW620 and human peripheral blood lymphocytes (PBL). SiFeNP were obtained by laser ablation and divided into the NP1 and NP2 fractions of 100 and 150 nm size, respectively. Cytotoxicity, apoptosis induction, reactive oxygen species (ROS) production, and lysosome metabolism were analyzed using in vitro methods. EC were found to efficiently incytosed both types of NPs, which resulted in the increase in the granularity of cells. NP did not cause apoptosis or EC necrosis, but accumulated in lysosomes, which led to a decrease in the membrane potential of lysosomes. In turn, a decrease in the level of EC metabolism led to a gradual (24 h) increase in ROS production by 10-15%. NP1 caused more ROS than NP2, and accumulated more in the EC, which may be the result of a difference in the particle size. SiFeNP did not interact with PBL. Thus, the total cytotoxicity of SiFeNP did not exceed 20%, which is associated with a decrease in lysosome metabolism and insignificant ROS production.
- ПубликацияТолько метаданныеStudy of IR Photoheating of Aqueous Solutions of Boron Nanoparticles Synthesized by Pulsed Laser Ablation for Cancer Therapy(2022) Belyaev, I. B.; Zelepukin, I. V.; Pastukhov, A. I.; Shakhov, P. V.; Tikhonowski, G. V.; Popov, A. A.; Zakharkiv, A. Y.; Klimentov, S. M.; Garmash, A. A.; Zavestovskaya, I. N.; Deyev, S. M.; Kabashin, A. V.; Шахов, Павел Владимирович; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Захаркив, Анастасия Юрьевна; Климентов, Сергей Михайлович; Гармаш, Александр Александрович; Завестовская, Ирина Николаевна; Деев, Сергей МихайловичAbstract: Photothermal properties of boron (B) nanoparticles (NPs) synthesized by pulsed laser ablation in liquid (PLAL) are studied. It is found that B NPs have high optical extinction coefficient in the near infrared (IR) region, in particular, its value was 0.2 l/(cm g) at a wavelength of 800 nm. It is shown that aqueous suspensions of B NPs can be heated during laser irradiation with a wavelength of 830 nm and a radiation power of 1 W. Here, the temperature increase varied from 6.5 to 36°C under the increase of the particle concentration from 0.04 to 0.8 g/L; while the relevant parameter in the absence of particles was 2°C. B NPs were able to provide a stable photothermal response during several irradiation cycles. The results evidence a high potential of B NPs as sensitizers of photothermal therapy (PTT). © 2022, Allerton Press, Inc.
- ПубликацияТолько метаданныеQuantitative Assessment of the Quality of Remote Irradiation Planning for Cancer Patients(2022) Lebedenko, I. M.; Kudashkina, Y. A.; Gromushkina, E. V.; Zakharkiv, A. Y.; Лебеденко, Ирина Матвеевна; Громушкина, Елена Вячеславовна; Захаркив, Анастасия ЮрьевнаRelationships between the index HI of dose homogeneity within the target and irradiation technique were obtained for patients with four types of cancer: cervical cancer, lung cancer, prostate cancer, and breast cancer. Homogeneity indexes HI were evaluated. The optimum irradiation techniques were selected for patients with each type of cancer taking account of the prognostic capabilities of the index HI for recurrence-free survival. These studies showed in particular that 3D-conformal radiotherapy (3DCRT) and intensity-modulated radiotherapy (IMRT) were suitable for treatment of cervical cancer. Intensity-modulated radiotherapy in the rotary mode (RapidArc) had advantages in lung cancer. The RapidArc technique was best in prostate cancer. The 3DCRT technique run on conventional accelerators was preferred in breast cancer. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
- ПубликацияТолько метаданныеCarbon Nanodots Obtained by Microwave Synthesis: Physical Properties and Assessment of Cytotoxicity on a Model of Glioblastoma and Embryonal Kidney In Vitro(2023) Kopylov, A. N.; Musaeva, D. U.; Alekseeva, A. I.; Zakharkiv, A. Y.; Timoshenko, V. Y.; Копылов, Алексей Николаевич; Мусаева, Дария Уланбековна; Захаркив, Анастасия Юрьевна; Тимошенко, Виктор Юрьевич
- ПубликацияОткрытый доступPeculiarities of biomedical applications of silicon nanoparticles (Review)(2020) Egorova, A. M.; Klimentov, S. M.; Oleinikov, V. A.; Fayzullaeva, S.; Karpov, N. V.; Zakharkiv, A. Yu.; Tikhonovsky, G. V.; Климентов, Сергей Михайлович; Олейников, Владимир Александрович; Файзуллаева, Сабина Юсуповна; Захаркив, Анастасия Юрьевна; Тихоновский, Глеб Валерьевич© Published under licence by IOP Publishing Ltd.The review highlights today directions in biomedical applications of silicon nanoparticles for the tasks of early diagnostics and minimum invasive treatment of cancer. It reveals the important issues related to their biocompatibility, toxicity, biodegradation, functionalization and targeting of drugs carried by porous silicon nanocontainers, making stress on theranostic applications of this novel material.