Персона: Лощенов, Виктор Борисович
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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- ПубликацияОткрытый доступThe engraftment dynamics evaluation of skin grafts via aluminium phthalocyanine nanoparticles using spectroscopic methods(2019) Makarov, V. I.; Farrakhova, D. S.; Loschenov, V. B.; Лощенов, Виктор Борисович© Published under licence by IOP Publishing Ltd.The research and comparison of spectroscopic methods for assessment of skin grafts engraftment/rejection after the back cross skin transplantation of laboratory mice were carried out. The spectral analysis was performed via inflammatory reaction evaluation of the studied area. The inflammatory reaction intensity correlates with the fluorescence intensity of the aluminum phthalocyanine nanoparticles. The photodynamic therapy was carried out to improve the skin engraftment and reduce the inflammatory reaction.
- ПубликацияОткрытый доступEffect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro(2024) Makarov, V. I.; Skobeltsin, A. S.; Averchuk, A. S.; Loschenov, V. B.; Макаров, Владимир Игоревич; Скобельцин, Алексей Сергеевич; Лощенов, Виктор БорисовичBackground: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. Methods: The study was performed on the primary culture of CECs. The cells underwent photodynamic treatment through 660 nm laser irradiation at a power density of 300 mW/cm2. The MTT, TMRE, and TUNEL assays were used to assess the survival, redox metabolism, mitochondrial activity, and apoptosis of CECs. Additionally, the metabolic activity of cells was evaluated using FLIM by measuring the fluorescence lifetime of NADH and FAD. Results: When CECs were incubated with MB, there was an increase in mitochondrial activity that was dependent on the concentration of MB. Additionally, mitochondrial activity increased when the CECs were exposed to 660 nm laser irradiation at an energy dose of up to 5 J/cm2. Following PDT, a slight shift towards oxidative phosphorylation was observed. Conclusions: In vitro application of MB accumulation or laser irradiation causes a shift in the redox status of CECs towards increased reducing activity, without causing any cell damage. However, the combined action of PS and laser radiation has the opposite effect on the redox status of cells, resulting in an increase in the oxidized form of FAD.
- ПубликацияТолько метаданныеSpectral properties of crystalline aluminum phthalocyanine nanoparticles and the possibility of their use in biophotonics(2024) Makarov, V. I.; Pominova, D. V.; Ryabova, A. V.; Loschenov, V. B.; Макаров, Владимир Игоревич; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
- ПубликацияТолько метаданныеEvaluating the dynamics of brain tissue oxygenation using near-infrared spectroscopy on various experimental models(2019) Kustov, D. M.; Sharova, A. S.; Makarov, V. I.; Borodkin, A. V.; Savelieva, T. A.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович© 2019 Astro Ltd.In this paper we consider a method for researching the dynamics of blood flow in the cerebral cortex, on an optical phantom that reproduces the parameters of real human and mice brain structures, through the use of near and infrared ranges of laser radiation. For the investigation of real tissue we chose a laboratory mouse brain in vivo. An algorithm for non-invasive diagnostics of the degree of oxygenation was identified and optimal parameters of installation components were selected for taking information about hemodynamic indicators. Output was verified by the reference method for assessing oxygenation by degree of absorption of hemoglobin in the visible range, which indicates that data have a high correlation with classical methods. With further development, this algorithm can be used in various areas of research and diagnostics.
- ПубликацияТолько метаданныеCorrelation of synovial caspase-3 concentration and the photodynamic effectiveness in osteoarthritis treatment(2020) Zharova, T. A.; Kogan, E. A.; Makarov, V. I.; Smorchkov, M. M.; Loschenov, V. B.; Лощенов, Виктор Борисович© 2020 Elsevier B.V.Background: The present study focuses on investigation of Intra-articular PDT mechanisms for OA treatment. Also, a search for determination of the most effective dose of chlorin e6 (Ce6) for anti-inflammatory PDT of OA was carried out. Methods: The study was carried out on laboratory animals (11 Chinchilla rabbits, 1 year, 2.5 kg) with a gonarthritis model of post-traumatic OA. According to the instructions for using Photoditazin (Ce6 based PS) for PDT of human oncological and non-oncological diseases, the recommended dose is 0.7–1.2 mg/kg. For studies on rabbits, taking into account the conversion coefficient (3.2), the PS doses of 2.4, 3.2 and 6.4 mg/kg were selected. Fluorescence spectra were measured intra-articular before and after PDT using spectrometer with fiber-optic probe. The intrajoint PDT was carried out using a laser (662 ± 10 nm) and a fiber-optic catheter with a cylindrical diffuser inside a sapphire needle for a uniform distribution of the laser radiation. The immunohistochemical study was carried out by staining the samples with caspase-3. Results: Histological and immunohistochemical analysis showed that the best PS dose for intravenous administration for PDT of rabbit gonarthritis is 3.2 mg/kg. The PS concentration directly in the synovial tissue was 0.5 mg/kg, and this was enough to achieve the most positive results to reduce the caspase-3 level. Conclusion: The caspase-3 level correlates well with other signs of inflammation in the synovial membrane (edema, etc.). Therefore, to assess the PDT effectiveness in the treatment of gonarthritis accompanied by synovitis, it is sufficient to analyze only for caspase-3. The efficacy of PDT with Ce6 showed that 3.2 mg/kg PS dose (1 mg/kg for a human) is the most effective.
- ПубликацияОткрытый доступCombined video analysis of ICG and 5-ALA induced protoporphyrin IX and hemoglobin oxygen saturation in near infrared(2019) Kustov, D. M.; Makarov, V. I.; Osipova, E. E.; Savelieva, T. A.; Grachev, P. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Лощенов, Виктор Борисович© 2019 by SCITEPRESS - Science and Technology Publications, Lda. All rights reserved Due to the high recurrence rate after the glial brain tumor removal, methods of intraoperative navigation have a high relevance, providing the most complete removal of tumor tissues with maximum preservation of healthy ones. In this work a combined visualization method is proposed with an assessment of fluorescence and diffuse reflectance images. Fluorescence intensity of 5-ALA-induced protoporphyrin IX allows visualization of tumor cells, distribution of indocyanine green fluorescence helps to visualize the vascular system of the tumor, and parallel mapping of the degree of oxygenation demonstrate the hypoxic regions. The images were obtained in the near infrared range of the optical spectrum in order to maximize the optical probing depth in the window of biological transparency.
- ПубликацияТолько метаданныеTheranostic Properties of Crystalline Aluminum Phthalocyanine Nanoparticles as a Photosensitizer(2022) Makarov, V. I.; Pominova, D. V.; Ryabova, A. V.; Romanishkin, I. D.; Voitova, A. V.; Steiner, R. W.; Loschenov, V. B.; Макаров, Владимир Игоревич; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор БорисовичThe study of phthalocyanines, known photosensitizers, for biomedical applications has been of high research interest for several decades. Of specific interest, nanophotosensitizers are crystalline aluminum phthalocyanine nanoparticles (AlPc NPs). In crystalline form, they are water-insoluble and atoxic, but upon contact with tumors, immune cells, or pathogenic microflora, they change their spectroscopic properties (acquire the ability to fluoresce and become phototoxic), which makes them upcoming agents for selective phototheranostics. Aqueous colloids of crystalline AlPc NPs with a hydrodynamic size of 104 В± 54 nm were obtained using ultrasonic dispersal and centrifugation. Intracellular accumulation and localization of AlPc were studied on HeLa and THP-1 cell cultures and macrophages (M0, M1, M2) by fluorescence microscopy. Crystallinity was assessed by XRD spectroscopy. Time-resolved spectroscopy was used to obtain characteristic fluorescence kinetics of AlPc NPs upon interaction with cell cultures. The photodynamic efficiency and fluorescence quantum yield of AlPc NPs in HeLa and THP-1 cells were evaluated. After entering the cells, AlPc NPs localized in lysosomes and fluorescence corresponding to individual AlPc molecules were observed, as well as destruction of lysosomes and a rapid decrease in fluorescence intensity during photodynamic action. The photodynamic efficiency of AlPc NPs in THP-1 cells was almost 1.8-fold that of the molecular form of AlPc (Photosens). A new mechanism for the occurrence of fluorescence and phototoxicity of AlPc NPs in interaction with cells is proposed.
- ПубликацияТолько метаданныеSynthesis Strategy of Tetrapyrrolic Photosensitizers for Their Practical Application in Photodynamic Therapy(2022) Koifman, O. I.; Ageeva, T. A.; Kuzmina, N. S.; Ryabova, A. V.; Pominova, D. V.; Makarov, V. I.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Макаров, Владимир Игоревич; Лощенов, Виктор Борисович
- ПубликацияТолько метаданныеThe approach to optimization parameters of aluminium phthalocyanine-based nanophotosensitizers for phototheranostic(2020) Makarov, V. I.; Lukyanets, E. A.; Kiselyova, S. K.; Loschenov, V. B.; Лощенов, Виктор Борисович© 2020 IEEE.The use of crystalline nanoparticles of aluminium phthalocyanine (AlPc) as nanophotosensitizers for phototheranostic is a promising direction, but due to insufficient study of their properties depending on the method of production, size, ratio of solvent concentrations, the use of these compounds is not widely developed yet.The investigations of size dependence, absorption and fluorescence of AlPc nanoparticles on various particle production conditions were carried out, and optimal conditions for obtaining the most effective samples of initial solutions for their testing on cancer and immune cells were found.
- ПубликацияОткрытый доступOptical spectral diagnostics of the oxygenation level in periodontal tissues and photodynamic therapy using methylene blue in children with cerebral palsy(2023) Morozova, N. S.; Kozlitina, Iu. A.; Makarov, V. I.; Loschenov, V. B.; Макаров, Владимир Игоревич; Лощенов, Виктор БорисовичDental diseases occur in children with cerebral palsy three times higher than in healthy children. Low values of the unstimulated salivation rate (andamp;lt;0.3 ml per minute), pH and buffer capacity, changes in enzyme activity and sialic acid concentration, as well as increased saliva osmolarity and total protein concentration, which indicates impaired hydration, are the factors in the development of a gingiva disease in case of cerebral palsy. This leads to increased bacterial agglutination and the formation of acquired pellicle and biofilm, leading to the formation of dental plaque. There is a tendency toward an increase in the concentration of hemoglobin and a decrease in the degree of hemoglobin oxygenation, as well as an increase in the generation of reactive oxygen and nitrogen species. Photodynamic therapy (PDT) with the use of photosensitizer methylene blue improves blood circulation and the degree of oxygenation in periodontal tissues, as well as eliminates a bacterial biofilm. Analysis of back diffuse reflection spectra makes it possible to conduct non-invasive monitoring determine tissue areas with a low level of hemoglobin oxygenation for precision photodynamic exposure. Aim To improve the effectiveness of phototheranostics methods using, namely PDT with simultaneous optical-spectral control, for the treatment of gingivitis in children with complex dental and somatic status (cerebral palsy). Methods The study involved 15 children (6-18 y.o.) with various forms of cerebral palsy, in particular, spastic diplegia and atonic-astatic form and with gingivitis. The degree of hemoglobin oxygenation was measured in tissues before PDT and on the 12th day. PDT was performed using laser radiation (О» = 660 nm) with a power density of 150 mW/cm 2 with a five-minute application of 0.01% MB. The total light dose was 45 В± 15 J/cm 2 . For statistical evaluation of the results, a paired Student s t-test was used. Results The paper presents the results of phototheranostics using methylene blue in children with cerebral palsy. An increase in the level of hemoglobin oxygenation from 50 to 67% ( p andamp;lt; 0.001) and a decrease in blood volume in the microcirculatory bed of periodontal tissues were shown. Conclusion Photodynamic therapy methods with application of methylene blue make it possible to assess the state of the gingival mucosa tissue diseases objectively in real time, and to provide effective targeted therapy for gingivitis in children with cerebral palsy. There is a prospect that they can become widely used clinical methods.