2019, Linkov, K. G., Meerovich, G. A., Akhlyustina, E. V., Savelieva, T. A., Loschenov, V. B., Меерович, Геннадий Александрович, Ахлюстина, Екатерина Витальевна, Савельева, Татьяна Александровна, Лощенов, Виктор Борисович

© 2019 Russian Photodynamic Association. All Rights Reserved. Currently, the most promising method for the study of pharmacokinetics of drugs with fluorescent properties is the spectral-fluorescent method. In this article, we propose an algorithm for expanding the dynamic range of the spectrum analyzer by automatically monitoring the maximum spectral density in the recorded fluorescence spectrum and automatically controlled changes in the accumulation time depending on this value, followed by compensation of the output signal with regard to this change, as well as hardware circuit solutions that allow this algorithm. Testing of LESA-01-"Biospeс" spectrum analyzer, upgraded using the proposed approach, was carried out on photosensitizer dispersions based on tetra-3-phenylthiophthalocyanine hydroxyaluminium of various concentrations (from 0.01 mg/l to 50 mg/l), approximately corresponding to the concentrations realized in the process of studying pharmacokinetics in calibration samples and tissues of experimental animals. The proposed solutions that implement the algorithm for recording fluorescence spectra with automatic change of accumulation time depending on the signal level, ensured a significant expansion of the dynamic range of the spectrum analyzer (up to 3.5 orders of magnitude) and improved accuracy in pharmacokinetic studies.

2019, Tiganova, I. G., Lukyanets, E. A., Makarova, E. A., Tolordava, E. R., Meerovich, G. A., Akhlyustina, E. V., Gonchukov, S. A., Loschenov, V. B., Меерович, Геннадий Александрович, Ахлюстина, Екатерина Витальевна, Гончуков, Сергей Александрович, Лощенов, Виктор Борисович

© 2019 Astro Ltd.The treatment of infected, long-term, non-healing, complicated wounds of the skin and mucosa, trophic ulcers, pressure sores and ulcers of diabetic feet represents a serious problem, especially in the case of resistant and multi-resistant pathogens. Antibacterial photodynamic therapy can be a promising method of local infected foci treatment of such diseases. This work is devoted to the study of the possibility of photodynamic inactivation (PDI) of Pseudomonas aeruginosa bacteria in the form of biofilms with the help of new polycationic photosensitizers (PSs) based on octacationic phthalocyanine and tetra-and octabacteriochlorins (ZnPcChol8, (3-PyBrE)4BCBr4 and (3-PyEPy)4BCBr8). More specifically, this work aims to clarify the role of light irradiation in PDI with the participation of new PSs, which has not been fully studied, especially with respect to the comparison of different PS types.

2019, Tiganova, I. G., Makarova, E. A., Philipova, N. I., Romanishkin, I. D., Akhlyustina, E. V., Meerovich, G. A., Loschenov, V. B., Ахлюстина, Екатерина Витальевна, Меерович, Геннадий Александрович, Лощенов, Виктор Борисович

© 2019 Published under licence by IOP Publishing Ltd. Polycationic derivatives of synthetic bacteriochlorin with absorption in the near infrared range are promising for the creation of photosensitizers (PS) for antimicrobial photodynamic therapy. In the present work, the photophysical and antibacterial properties of PS based on tetracationic derivatives of synthetic bacteriochlorins: hydrophilic 3-Py 4 BC(EtBr) 4 Br4 in aqueous solution and amphiphilic 3-Py 4 BCHP 4 Br 4 in the dispersion of Kolliphor ELP were studied. Analysis of absorption and fluorescence in a wide range of concentrations has demonstrated low aggregation of the PS over the entire range. A high efficiency of photodynamic inactivation of Gram-positive Staphylococcus aureus and Gramnegative Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii bacteria was observed.