Персона:
Блинова, Екатерина Валериевна

Научные группы

Научная группа

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

Фамилия

Блинова

Имя

Екатерина Валериевна

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Теперь показываю 1 - 10 из 14

Только метаданные
Expression of p53 protein associates with anti-pd-l1 treatment response on human-derived xenograft model of gata3/cr5/6-negative recurrent nonmuscular invasive bladder urothelial carcinoma
(2021) Samishina, E.; Deryabina, O.; Blinov, D.; Roshchin, D.; Blinova, E.; Блинова, Екатерина Валериевна
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.Background: The possible involvement of p53 signaling, FGFR3 expression, and FGFR3 mutation rates in the prediction of the NMIBC anti-PD-L1 treatment response needs to be clarified. The main aim of our study was to explore predictive value of p53 expression, FGFR3 expression, and its gene mutation status for the therapeutic success of anti-PD-L1 treatment in the patient-derived murine model of recurrent high-PD-L1(+) GATA3(-)/CR5/6(-) high-grade and low-grade NMIBC. Methods: twenty lines of patient-derived xenografts (PDXs) of relapsed high-PD-L1(+) double-negative NMIBC were developed, of which 10 lines represented high-grade tumors and the other ones—low-grade bladder cancer. Acceptors of each grade-related branch received specific anti-PD-L1 antibodies. Animals’ survival, tumor-doubling time, and remote metastasis were followed during the post-interventional period. PD-L1, GATA3, CR5/6, and p53 protein expressions in engrafted tumors were assessed by immunohistochemistry. The FGFR3 expression and FGFR3 mutations in codons 248 and 249 were detected by real-time polymerase chain reaction. Results: The expression of p53 protein is an independent factor affecting the animals’ survival time [HR = 0.036, p = 0.031] of anti-PD-L1-treated mice with low-grade high-PD-L1(+) double-negative NMIBC PDX. The FGFR3 expression and FGFR3 mutation rate have no impact on the anti-PD-L1 treatment response in the interventional groups. Conclusions: p53 expression may be considered as a prognostic factor for the anti-PD-L1 treatment efficacy of low-grade high-PD-L1-positive GATA3(-)/CR5/6(-)relapsed noninvasive bladder cancer.
Только метаданные
Novel Hydroxypyridine Compound Protects Brain Cells against Ischemic Damage In Vitro and In Vivo
(2022) Blinova, E.; Vasilkina, O.; Belanov, K.; Zalogin, S.; Blinov, D.; Блинова, Екатерина Валериевна
A non-surgical pharmacological approach to control cellular vitality and functionality during ischemic and/or reperfusion-induced phases of strokes remains extremely important. The synthesis of 2-ethyl-6-methyl-3-hydroxypyridinium gammalactone-2,3-dehydro-L-gulonate (3-EA) was performed using a topochemical reaction. The cell-protective effects of 3-EA were studied on a model of glutamate excitotoxicity (GluTox) and glucose-oxygen deprivation (OGD) in a culture of NMRI mice cortical cells. Ca2+ dynamics was studied using fluorescent bioimaging and a Fura-2 probe, cell viability was assessed using cytochemical staining with propidium iodide, and gene expression was assessed by a real-time polymerase chain reaction. The compound anti-ischemic efficacy in vivo was evaluated on a model of irreversible middle cerebral artery (MCA) occlusion in Sprague-Dawley male rats. Brain morphological changes and antioxidant capacity were assessed one week after the pathology onset. The severity of neurological disorder was evaluated dynamically. 3-EA suppressed cortical cell death in a dose-dependent manner under the excitotoxic effect of glutamate and ischemia/reoxygenation. Pre-incubation of cerebral cortex cells with 10–100 µM 3-EA led to significant stagnation in Ca2+ concentration in a cytosol ([Ca2+]i) of neurons and astrocytes suffering GluTox and OGD. Decreasing intracellular Ca2+ and establishing a lower [Ca2+]i baseline inhibited necrotic cell death in an acute experiment. The mechanism of 3-EA cytoprotective action involved changes in the baseline and ischemia/reoxygenation-induced expression of genes encoding anti-apoptotic proteins and proteins of the oxidative status; this led to inhibition of the late irreversible stages of apoptosis. Incubation of brain cortex cells with 3-EA induced an overexpression of the anti-apoptotic genes BCL-2, STAT3, and SOCS3, whereas the expression of genes regulating necrosis and inflammation (TRAIL, MLKL, Cas-1, Cas-3, IL-1β and TNFa) were suppressed. 3-EA 18.0 mg/kg intravenous daily administration for 7 days following MCA occlusion preserved rats’ cortex neuron population, decreased the severity of neurological deficit, and spared antioxidant capacity of damaged tissues. 3-EA demonstrated proven short-term anti-ischemic activity in vivo and in vitro, which can be associated with antioxidant activity and the ability to target necrotic and apoptotic death. The compound may be considered a potential neuroprotective molecule for further pre-clinical investigation. © 2022 by the authors.
Только метаданные
Experimental postoperative skin wound healing after Z-grafting followed by local application of cerium-containing N-acetyl-6-aminohexanoic acid compound
(2022) Galichenko, K. A.; Blinova, E.; Блинова, Екатерина Валериевна
Только метаданные
3-HYDROXYPYRIDINE DERIVATIVE WITH ASCORBIC ACID RESIDUE INCREASES SURVIVAL OF CEREBRAL CORTEX CELLS UNDER CONDITIONS OF EXPERIMENTAL ISCHEMIA IÐIECAIAIIA 3-AEAÐIENEIEÐEAEIA N INOAOEII ANEIÐAEIIAIE EENEIOU IIAUØAAO AUÆEAAAIINOU EEAOIE AIEIAIIAI IICAA A ONEIAEßO YENIAÐEIAIOAEUIIE EØAIEE
(2023) Blinova, E. V.; Timoshkin, D. E.; Belanov, K. Y.; Vasilkina, O. V.; Блинова, Екатерина Валериевна
Только метаданные
Buccal fat pad’ buccal flap anatomical variation
(2023) Mirontsev, A. V.; Kolesova, L. Yu.; Vasil’ev, Yu. L.; Blinova, E. V.; Миронцев, Артём Владимирович; Блинова, Екатерина Валериевна
Только метаданные
Clinical and experimental rationale for using phenylephrine with hypromellose for the treatment of extra accommodation strain in patients with myopia
(2023) Makhova, M. V.; Shikh, E. V.; Strakhov, V. V.; Blinova, E. V.; Blinov, D. S.; Блинова, Екатерина Валериевна
Только метаданные
Modification and Validation of a Laboratory Method for Determination of L-Asparaginase Activity in Patient Blood Serum
(2023) Osipiantz, A. I.; Shikalov, A. B.; Tumutolova, O. M.; Karachunsky, A. I.; Blinova, E. V.; Блинова, Екатерина Валериевна
Только метаданные
Antitumor activity of aminochromene derivative on the human-derived lung adenocarcinoma xenograft model in BALB/C nu/nu mice IÐIOEAIIIOOIEAAIA AAENOAEA IÐIECAIAIIAI AIEIIOÐIIAIA IA IIAAEE ENAIIAÐAOOA AAAIIEAÐOEIIIU EAAEIAI O IUØAE nu/nu BALB
(2021) Dudina, M. O.; Blinov, D. S.; Suslova, I. R.; Skachilova, S. Ya.; Blinova, E. V.; Блинова, Екатерина Валериевна
© 2021 Izdatel'stvo Meditsina. All rights reserved.The antitumor and anti-metastatic effects of aminochromene derivative AKh-554 upon intragastric administration were studied on immunodeficient BALB/c nu/nu female mice with heterotopic human-derived lung adenocarcinoma xenograft model. In the tumor tissue of animals treated with the test compound, the levels of TUBB3, ALK and c-MET/HFG oncomarkers were quantitatively detected and it was found that the drug suppressed the velocity of tumor growth and its size as well as exhibited anti-metastatic activity. AKh-554 produced 3.3-fold increase in tumor carriers survival and induced stable remission in 60% of test mice (p 0.001). The effect might be due to anti-ALK-dependent activation of apoptosis of tumor cells and suppression of cell proliferation. The results were obtained on the tumor system without signs of pharmacoresistance to AX-554, which was confirmed by the lack of dynamics of c-MET/HFG in tumor tissue in all test groups.
Только метаданные
Synthesis and In Silico Prediction of the Molecular-Targeting Anti-EGFR Action of a Novel Dihydroacridinone
(2024) Epishkina, A. A.; Bogoslovskaya, E. V.; Pakina, V. A.; Blinova, E. V.; Епишкина, Анна Алексеевна; Блинова, Екатерина Валериевна
Только метаданные
A novel dihydroacridine derivative targets epidermal growth factor receptor-expressing cancer cells in vitro and in vivo
(2024) Epishkina, A.; Pakina, V.; Kutorkina, E.; Bogoslovskaya, E.; Blinova, E.; Блинова, Екатерина Валериевна