Properties of tench - Tinca tinca L. sperm and experiments with freezing it at -196°C
Дата
Авторы
Moczarski,M.
Kołdras,M.
Journal Title
Journal ISSN
Volume Title
Издатель
Pensoft Publishers
Аннотация
Описание
Properties of tench sperm were studied by means of the following indices: sperm volume, spermatozoa concentration, total number of spermatozoa, spermatocrit, and sperm motility. A series of diluting media was tested in order to find optimal conditions for tench sperm freezing; phosphate buffer (BF) enriched by a 15% addition of ethylene glycol (EG) was found to be the most suitable diluting medium.