Publication:
Boron Nanoparticle-Enhanced Proton Therapy: Molecular Mechanisms of Tumor Cell Sensitization

Простая страница элемента
dc.contributor.authorPopov, A. L.
dc.contributor.authorKolmanovich, D. D.
dc.contributor.authorChukavin, N. N.
dc.contributor.authorZelepukin, I. V.
dc.contributor.authorTikhonowski, G. V.
dc.contributor.authorPopov, A. A.
dc.contributor.authorKlimentov, S. M.
dc.contributor.authorDeyev, S. M.
dc.contributor.authorZavestovskaya, I. N.
dc.contributor.authorТихоновский, Глеб Валерьевич
dc.contributor.authorПопов, Антон Александрович
dc.contributor.authorКлиментов, Сергей Михайлович
dc.contributor.authorДеев, Сергей Михайлович
dc.contributor.authorЗавестовская, Ирина Николаевна
dc.date.accessioned2024-12-04T13:21:23Z
dc.date.available2024-12-04T13:21:23Z
dc.date.issued2024
dc.description.abstractBoron-enhanced proton therapy has recently appeared as a promising approach to increase the efficiency of proton therapy on tumor cells, and this modality can further be improved by the use of boron nanoparticles (B NPs) as local sensitizers to achieve enhanced and targeted therapeutic outcomes. However, the mechanisms of tumor cell elimination under boron-enhanced proton therapy still require clarification. Here, we explore possible molecular mechanisms responsible for the enhancement of therapeutic outcomes under boron NP-enhanced proton therapy. Spherical B NPs with a mode size of 25 nm were prepared by methods of pulsed laser ablation in water, followed by their coating by polyethylene glycol to improve their colloidal stability in buffers. Then, we assessed the efficiency of B NPs as sensitizers of cancer cell killing under irradiation with a 160.5 MeV proton beam. Our experiments showed that the combined effect of B NPs and proton irradiation induces an increased level of superoxide anion radical generation, which leads to the depolarization of mitochondria, a drop in their membrane mitochondrial potential, and the development of apoptosis. A comprehensive gene expression analysis (via RT-PCR) confirmed increased overexpression of 52 genes (out of 87 studied) involved in the cell redox status and oxidative stress, compared to 12 genes in the cells irradiated without B NPs. Other possible mechanisms responsible for the B NPs-induced radiosensitizing effect, including one related to the generation of alpha particles, are discussed. The obtained results give a better insight into the processes involved in the boron-induced enhancement of proton therapy and enable one to optimize parameters of proton therapy in order to maximize therapeutic outcomes.
dc.identifier.citationBoron Nanoparticle-Enhanced Proton Therapy: Molecular Mechanisms of Tumor Cell Sensitization / Popov, A.L. [et al.] // Molecules. - 2024. - 29. - № 16. - 10.3390/molecules29163936
dc.identifier.doi10.3390/molecules29163936
dc.identifier.urihttps://www.doi.org/10.3390/molecules29163936
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85202625571&origin=resultslist
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/26243
dc.relation.ispartofMolecules
dc.subjectProton Therapy
dc.subjectBoron Neutron Capture Therapy
dc.subjectNanoparticle Radiosensitization
dc.subjectRadiation Sensitization
dc.subjectCancer Treatment
dc.titleBoron Nanoparticle-Enhanced Proton Therapy: Molecular Mechanisms of Tumor Cell Sensitization
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue16
oaire.citation.volume29
relation.isAuthorOfPublication868a437b-a6d5-414b-81ce-0ea5dda957a7
relation.isAuthorOfPublicationba484d03-e187-4fef-bd0d-7c345c3c6061
relation.isAuthorOfPublication08d6d476-247c-4c17-958a-8c1173dd1f60
relation.isAuthorOfPublication66f50b35-0e92-49e0-ad1a-0ac386d858b7
relation.isAuthorOfPublication1a1d2a9c-068f-48c6-b18a-7da3ce75cf7a
relation.isAuthorOfPublication.latestForDiscovery868a437b-a6d5-414b-81ce-0ea5dda957a7
relation.isOrgUnitOfPublicationc8407a6f-7272-450d-8d99-032352c76b55
relation.isOrgUnitOfPublication.latestForDiscoveryc8407a6f-7272-450d-8d99-032352c76b55
Файлы
Коллекции
Публикации