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Cancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules

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dc.contributor.authorRamos-Gomes, Fernanda
dc.contributor.authorAlves, Frauke
dc.contributor.authorNifontova, Galina
dc.contributor.authorBaryshnikova, Maria
dc.contributor.authorNabiev, Igor
dc.contributor.authorSukhanova, Alyona
dc.contributor.authorНифонтова, Галина Олеговна
dc.contributor.authorБарышникова, Мария Анатольевна
dc.contributor.authorНабиев, Игорь Руфаилович
dc.contributor.authorСуханова, Алена Владимировна
dc.date.accessioned2024-11-20T09:26:41Z
dc.date.available2024-11-20T09:26:41Z
dc.date.issued2019
dc.description.abstractImaging agents and drug carriers are commonly targeted toward cancer cell through functionalization with specific recognition molecules. Quantum dots (QDs) are fluorescent semiconductor nanocrystals whose extraordinary brightness and photostability make them attractive for direct fluorescent labeling of biomolecules or optical encoding of the membranes and cells. Here, we analyse the cytotoxicity of QD-encoded microcapsules, validate an approach to the activation of the microcapsule's surface for further functionalization with monoclonal antibody Trastuzumab, a humanized monoclonal antibody targeting the extracellular domain of the human epidermal growth factor receptor 2 (HER2) and already in clinical use for the treatment of HER2 positive breast cancer. In addition, we characterize the cell-specific targeting activity of the resultant bio-conjugate by immunofluorescence assay (IFA) and real-time analysis of interaction of the conjugates with live HER2 overexpressing human breast cancer cells. We demonstrate, that encapsulation of QDs into the polymer shell using the layer-by-layer deposition method yields highly fluorescent polyelectrolyte microcapsules with a homogeneous size distribution and biocompatibility upon in vitro treatment of cancer cells. Carbodiimide surface activation ensures optimal disperse and optical characteristics of the QD-encoded microcapsules before antibody conjugation. The prepared conjugates of the microcapsules with cancer-specific monoclonal antibody targeting HER2 provide sufficiently sensitive and specific antibody-mediated binding of the microcapsules with live cancer cells, which demonstrated their potential as prospective cancer cell-targeting agents.
dc.identifier.citationCancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules / Ramos-Gomes, Fernanda [et al.] // Frontiers in Chemistry. - 2019. - 7. - 10.3389/fchem.2019.00034
dc.identifier.doi10.3389/fchem.2019.00034
dc.identifier.urihttps://www.doi.org/10.3389/fchem.2019.00034
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85061199766&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000457223300001
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/16513
dc.relation.ispartofFrontiers in Chemistry
dc.titleCancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules
dc.typeArticle
dspace.entity.typePublication
oaire.citation.volume7
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