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Label-Free Multiplexed Microfluidic Analysis of Protein Interactions Based on Photonic Crystal Surface Mode Imaging

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dc.contributor.authorNifontova, G.
dc.contributor.authorPetrova, I.
dc.contributor.authorGerasimovich, E.
dc.contributor.authorNabiev, I.
dc.contributor.authorГерасимович, Евгения Семёновна
dc.contributor.authorНабиев, Игорь Руфаилович
dc.date.accessioned2024-12-28T09:24:53Z
dc.date.available2024-12-28T09:24:53Z
dc.date.issued2023
dc.description.abstractHigh-throughput protein assays are crucial for modern diagnostics, drug discovery, proteomics, and other fields of biology and medicine. It allows simultaneous detection of hundreds of analytes and miniaturization of both fabrication and analytical procedures. Photonic crystal surface mode (PC SM) imaging is an effective alternative to surface plasmon resonance (SPR) imaging used in conventional gold-coated, label-free biosensors. PC SM imaging is advantageous as a quick, label-free, and reproducible technique for multiplexed analysis of biomolecular interactions. PC SM sensors are characterized by a longer signal propagation at the cost of a lower spatial resolution, which makes them more sensitive than classical SPR imaging sensors. We describe an approach for designing label-free protein biosensing assays employing PC SM imaging in the microfluidic mode. Label-free, real-time detection of PC SM imaging biosensors using two-dimensional imaging of binding events has been designed to study arrays of model proteins (antibodies, immunoglobulin G-binding proteins, serum proteins, and DNA repair proteins) at 96 points prepared by automated spotting. The data prove feasibility of simultaneous PC SM imaging of multiple protein interactions. The results pave the way to further develop PC SM imaging as an advanced label-free microfluidic assay for the multiplexed detection of protein interactions.
dc.identifier.citationLabel-Free Multiplexed Microfluidic Analysis of Protein Interactions Based on Photonic Crystal Surface Mode Imaging / Nifontova, G. [et al.] // International Journal of Molecular Sciences. - 2023. - 24. - № 5. - 10.3390/ijms24054347
dc.identifier.doi10.3390/ijms24054347
dc.identifier.urihttps://www.doi.org/10.3390/ijms24054347
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85149858788&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000948140200001
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/30025
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.subjectMolecular binding
dc.subjectSurface Plasmons
dc.subjectBiomolecule
dc.subjectProtein microarray
dc.subjectMultiplexed Assays
dc.subjectNanophotonics
dc.titleLabel-Free Multiplexed Microfluidic Analysis of Protein Interactions Based on Photonic Crystal Surface Mode Imaging
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.volume24
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relation.isAuthorOfPublicationa4923e3c-317e-498d-a747-cad6d3054e23
relation.isAuthorOfPublication.latestForDiscovery9f7f1e2f-4388-41d0-b5ff-24cfac7c8009
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