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Highly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy

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dc.contributor.authorBragina, V. A.
dc.contributor.authorKhomyakova, E.
dc.contributor.authorOrlov, A. V.
dc.contributor.authorZnoyko, S. L.
dc.contributor.authorNikitin, P. I.
dc.contributor.authorНикитин, Петр Иванович
dc.date.accessioned2024-12-26T11:51:46Z
dc.date.available2024-12-26T11:51:46Z
dc.date.issued2022
dc.description.abstract© 2022 by the authors. Licensee MDPI, Basel, Switzerland.Extracellular vesicles (EVs) are promising agents for liquid biopsy—a non-invasive approach for the diagnosis of cancer and evaluation of therapy response. However, EV potential is limited by the lack of sufficiently sensitive, time-, and cost-efficient methods for their registration. This research aimed at developing a highly sensitive and easy-to-use immunochromatographic tool based on magnetic nanoparticles for EV quantification. The tool is demonstrated by detection of EVs isolated from cell culture supernatants and various body fluids using characteristic biomarkers, CD9 and CD81, and a tumor-associated marker—epithelial cell adhesion molecules. The detection limit of 3.7 × 105 EV/µL is one to two orders better than the most sensitive traditional lateral flow system and commercial ELISA kits. The detection specificity is ensured by an isotype control line on the test strip. The tool’s advantages are due to the spatial quantification of EV-bound magnetic nanolabels within the strip volume by an original electronic technique. The inexpensive tool, promising for liquid biopsy in daily clinical routines, can be extended to other relevant biomarkers.
dc.identifier.citationHighly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy / Bragina, V.A. [et al.] // Nanomaterials. - 2022. - 12. - № 9. - 10.3390/nano12091579
dc.identifier.doi10.3390/nano12091579
dc.identifier.urihttps://www.doi.org/10.3390/nano12091579
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85129428093&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000794629700001
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/29053
dc.relation.ispartofNanomaterials
dc.titleHighly Sensitive Nanomagnetic Quantification of Extracellular Vesicles by Immunochromatographic Strips: A Tool for Liquid Biopsy
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue9
oaire.citation.volume12
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