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Automated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies

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dc.contributor.authorEdouard, S.
dc.contributor.authorJaafar, R.
dc.contributor.authorOrain, N.
dc.contributor.authorParola, P.
dc.contributor.authorColson, P.
dc.contributor.authorLa Scola, B.
dc.contributor.authorFournier, P. -E.
dc.contributor.authorRaoult, D.
dc.contributor.authorDrancourt, M.
dc.date.accessioned2024-11-30T03:49:23Z
dc.date.available2024-11-30T03:49:23Z
dc.date.issued2021
dc.description.abstractELISA and chemiluminescence serological assays for COVID-19 are currently incorporating only one or two SARS-CoV-2 antigens. We developed an automated Western immunoblotting as a complementary serologic assay for COVID-19. The JessTM Simple Western system, an automated capillary-based assay, was used, incorporating an inactivated SARS-CoV-2 lineage 20a strain as the source of antigen, and total immunoglobulins (IgG, IgM, IgA) detection. In total, 602 sera were tested including 223 from RT-PCR-confirmed COVID-19 patients, 76 from patients diagnosed with seasonal HCoVs and 303 from coronavirus-negative control sera. We also compared this assay with the EUROIMMUN® SARS-CoV-2 IgG ELISA kit. Among 223 sera obtained from RT-PCR-confirmed COVID-19 patients, 180/223 (81%) exhibited reactivity against the nucleocapsid and 70/223 (31%) against the spike protein. Nucleocapsid reactivity was further detected in 9/76 (14%) samples collected from patients diagnosed with seasonal HCoVs and in 15/303 (5%) coronavirus-negative control samples. In the subset of sera collected more than 2 weeks after the onset of symptoms, the sensitivity was 94% and the specificity 93%, the latter value probably reflecting cross-reactivity of SARS-CoV-2 with other coronaviruses. The automated Western immunoblotting presented a substantial agreement (90%) with the compared ELISA (Cohen’s Kappa=0.64). Automated Western immunoblotting may be used as a second line test to monitor exposure of people to HCoVs including SARS-CoV-2. © 2021, The Author(s).
dc.format.extentС. 1309-1317
dc.identifier.citationAutomated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies / Edouard, S. [et al.] // European Journal of Clinical Microbiology and Infectious Diseases. - 2021. - 40. - № 6. - P. 1309-1317. - 10.1007/s10096-021-04203-8
dc.identifier.doi10.1007/s10096-021-04203-8
dc.identifier.urihttps://www.doi.org/10.1007/s10096-021-04203-8
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85106680665&origin=resultslist
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/25395
dc.relation.ispartofEuropean Journal of Clinical Microbiology and Infectious Diseases
dc.titleAutomated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue6
oaire.citation.volume40
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