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Label-Free Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

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dc.contributor.authorSarychev, A. K.
dc.contributor.authorSukhanova, A.
dc.contributor.authorIvanov, A. V.
dc.contributor.authorBykov, I. V.
dc.contributor.authorBakholdin, N. V.
dc.contributor.authorVasina, D. V.
dc.contributor.authorGushchin, V. A.
dc.contributor.authorTkachuk, A. P.
dc.contributor.authorNifontova, G.
dc.contributor.authorSamokhvalov, P. S.
dc.contributor.authorKaraulov, A.
dc.contributor.authorNabiev, I.
dc.contributor.authorНифонтова, Галина Олеговна
dc.contributor.authorСамохвалов, Павел Сергеевич
dc.contributor.authorНабиев, Игорь Руфаилович
dc.date.accessioned2024-12-25T07:49:42Z
dc.date.available2024-12-25T07:49:42Z
dc.date.issued2022
dc.description.abstractSurface-enhanced Raman scattering (SERS) spectroscopy is a surface-or cavity-enhanced variant of Raman scattering spectroscopy that allows the detection of analytes with a sensitivity down to single molecules. This method involves the use of SERS-active surfaces or cavities capable of concentrating incident radiation into small mode volumes containing the analyte. Here, we have engineered an ultranarrow metal–dielectric nano-cavity out of a film of the receptor-binding domain (RBD) of SARS-CoV-2 spike (S) glycoprotein and a silver surface, held together by interaction between reduced protein sulfhydryl groups and silver. The concentration of light in this nano-cavity allows the label-free recording of the characteristic Raman spectra of protein samples smaller than 1 pg. This is sufficient for the ultrasensitive detection of viral protein antigens at physiologically relevant levels. Moreover, the protein SERS signal can be increased by several orders of magnitude by coating the RBD film with a nanometer-thick silver shell, thereby raising the cavity Q-factor. This ensures a sub-femtogram sensitivity of the viral antigen detection. A simple theoretical model ex-plaining the observed additional enhancement of the SERS signal from the silver-coated protein is proposed. Our study is the first to obtain the characteristic Raman and SERS spectra of the RBD of S glycoprotein, the key SARS-CoV-2 viral antigen, directly, without the use of Raman-reporter mol-ecules. Thus, our approach allows label-free recording of the characteristic spectra of viral antigens at concentrations orders of magnitude lower than those required for detecting the whole virus in biological media. This makes it possible to develop a high-performance optical detection method and conformational analysis of the pathogen and its variants. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.identifier.citationLabel-Free Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy / Sarychev, A.K. [et al.] // Biosensors. - 2022. - 12. - № 5. - 10.3390/bios12050300
dc.identifier.doi10.3390/bios12050300
dc.identifier.urihttps://www.doi.org/10.3390/bios12050300
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85131106869&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000801520300001
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/27350
dc.relation.ispartofBiosensors
dc.titleLabel-Free Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.volume12
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