Publication: Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water-Ethanol Mixtures
Дата
2020
Авторы
Bunkin, N. F.
Shkirin, A. V.
Ninham, B. W.
Chirikov, S. N.
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Аннотация
Structural characterization by three complementary methods of laser diagnostics (d)nainic light scattering, laser phase microscopy, and laser polarimetric scatterometry) has established that shaking of immunoglobulin G (IgG) dispersions in water and ethanol-water mixtures (36.7 vol %) results in two effects. First, it intensifies the aggregation of IgG macromolecules. Second, it generates bubbles with a size range that is different in each solvent. The aggregation is enhanced in ethanol-water mixtures because of IgG denaturation. IgG aggregates have a size of similar to 300 nm in water and similar to 900 nm in ethanol-water mixtures. The flotation of IgG is much more efficient in water. This can be explained by a better adsorption of IgG particles (molecules and aggregates) on bubbles in water as compared to ethanol-water mixtures. Bulk nanobubbles and their association with IgG aggregates were visualized by laser phase microscopy in water but were not detected in ethanol-water mixtures. Therefore, the nanobubble flotation mechanism for IgG aggregates acting in water is not feasible for ethanol-water mixtures.
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Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water-Ethanol Mixtures / Bunkin, NF [et al.] // ACS Omega. - 2020. - 5. - № 24. - P. 14689-14701. - 10.1021/acsomega.0c01444
URI
https://www.doi.org/10.1021/acsomega.0c01444
https://www.scopus.com/record/display.uri?eid=2-s2.0-85087435061&origin=resultslist
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https://openrepository.mephi.ru/handle/123456789/21991
https://www.scopus.com/record/display.uri?eid=2-s2.0-85087435061&origin=resultslist
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000543740600051
https://openrepository.mephi.ru/handle/123456789/21991