Publication:
Photonic Crystal Surface Mode Real-Time Imaging of RAD51 DNA Repair Protein Interaction with the ssDNA Substrate

Простая страница элемента
dc.contributor.authorNifontova, G.
dc.contributor.authorCharlier, C.
dc.contributor.authorAyadi, N.
dc.contributor.authorNabiev, I.
dc.contributor.authorНабиев, Игорь Руфаилович
dc.date.accessioned2024-12-04T08:37:25Z
dc.date.available2024-12-04T08:37:25Z
dc.date.issued2024
dc.description.abstractPhotonic crystals (PCs) are promising tools for label-free sensing in drug discovery screening, diagnostics, and analysis of ligandў??receptor interactions. Imaging of PC surface modes has emerged as a novel approach to the detection of multiple binding events at the sensor surface. PC surface modification and decoration with recognition units yield an interface providing the highly sensitive detection of cancer biomarkers, antibodies, and oligonucleotides. The RAD51 protein plays a central role in DNA repair via the homologous recombination pathway. This recombinase is essential for the genome stability and its overexpression is often correlated with aggressive cancer. RAD51 is therefore a potential target in the therapeutic strategy for cancer. Here, we report the designing of a PC-based array sensor for real-time monitoring of oligonucleotideў??RAD51 recruitment by means of surface mode imaging and validation of the concept of this approach. Our data demonstrate that the designed biosensor ensures the highly sensitive multiplexed analysis of associationў??dissociation events and detection of the biomarker of DNA damage using a microfluidic PC array. The obtained results highlight the potential of the developed technique for testing the functionality of candidate drugs, discovering new molecular targets and drug entities. This paves the way to further adaption and bioanalytical use of the biosensor for high-content screening to identify new DNA repair inhibitor drugs targeting the RAD51 nucleoprotein filament or to discover new molecular targets.
dc.identifier.citationPhotonic Crystal Surface Mode Real-Time Imaging of RAD51 DNA Repair Protein Interaction with the ssDNA Substrate / Nifontova, G. [et al.] // Biosensors. - 2024. - 14. - № 1. - 10.3390/bios14010043
dc.identifier.doi10.3390/bios14010043
dc.identifier.urihttps://www.doi.org/10.3390/bios14010043
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85183082002&origin=resultslist
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/25784
dc.relation.ispartofBiosensors
dc.subjectReal-Time PCR
dc.subjectCancer diagnostics
dc.titlePhotonic Crystal Surface Mode Real-Time Imaging of RAD51 DNA Repair Protein Interaction with the ssDNA Substrate
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.volume14
relation.isAuthorOfPublicationa4923e3c-317e-498d-a747-cad6d3054e23
relation.isAuthorOfPublication.latestForDiscoverya4923e3c-317e-498d-a747-cad6d3054e23
relation.isOrgUnitOfPublicationc8407a6f-7272-450d-8d99-032352c76b55
relation.isOrgUnitOfPublication.latestForDiscoveryc8407a6f-7272-450d-8d99-032352c76b55
Файлы
Original bundle
Теперь показываю 1 - 1 из 1
Уменьшенное изображение
Name:
W4390878698.pdf
Size:
2.7 MB
Format:
Adobe Portable Document Format
Description:
Download
Коллекции
Публикации