Publication:
Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction

Файлы
060501_1.pdf(2.96 MB)
Дата
2019
Авторы
Kalchenko, V.
Sdobnov, A.
Kuznetsov, Y.
Harmelin, A.
Meglinski, I.
Journal Title
Journal ISSN
Volume Title
Издатель
Научные группы
Организационные подразделения
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Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
Выпуск журнала
Аннотация
(2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).Optical clearing agents (OCAs) and many chemicals are widely used in functional diagnosis of skin tissues. Numerous studies are associated with the transcutaneous diffusion of OCA in epidermal, dermal, and hypodermal tissues, which results in changing their optical properties. In addition, an objective approach that is suitable for screening the influence of utilized OCA, as well as various chemical agents, synthetics, and nanomaterials, on blood and lymph flows is highly desirable. In our study, a highly sensitive laser speckle imaging (LSI) system and fluorescent intravital microscopy (FIM) were used team-wise to inspect the acute skin vascular permeability reaction in mouse ear during the local application of OCA on the skin surface. Fluorescent contrast material administrated intravenously was used for quantitatively assessing the intensity of vascular permeability reaction and the strength of skin irritation. The obtained results suggest that a combined use of LSI and FIM is highly effective for monitoring the cutaneous vascular permeability reaction, with great potential for assessment of allergic reactions of skin in response to interactions with chemical substances.
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Цитирование
Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction / Kalchenko,V. [et al.] // Journal of Biomedical Optics. - 2019. - 24. - 6. - N 1-4. - 10.1117/1.JBO.24.6.060501
URI
https://www.doi.org/10.1117/1.JBO.24.6.060501
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85066934572&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000481881900001
 https://openrepository.mephi.ru/handle/123456789/15915
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