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Effect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro

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dc.contributor.authorMakarov, V. I.
dc.contributor.authorSkobeltsin, A. S.
dc.contributor.authorAverchuk, A. S.
dc.contributor.authorLoschenov, V. B.
dc.contributor.authorМакаров, Владимир Игоревич
dc.contributor.authorСкобельцин, Алексей Сергеевич
dc.contributor.authorЛощенов, Виктор Борисович
dc.date.accessioned2024-12-04T11:01:43Z
dc.date.available2024-12-04T11:01:43Z
dc.date.issued2024
dc.description.abstractBackground: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. Methods: The study was performed on the primary culture of CECs. The cells underwent photodynamic treatment through 660 nm laser irradiation at a power density of 300 mW/cm2. The MTT, TMRE, and TUNEL assays were used to assess the survival, redox metabolism, mitochondrial activity, and apoptosis of CECs. Additionally, the metabolic activity of cells was evaluated using FLIM by measuring the fluorescence lifetime of NADH and FAD. Results: When CECs were incubated with MB, there was an increase in mitochondrial activity that was dependent on the concentration of MB. Additionally, mitochondrial activity increased when the CECs were exposed to 660 nm laser irradiation at an energy dose of up to 5 J/cm2. Following PDT, a slight shift towards oxidative phosphorylation was observed. Conclusions: In vitro application of MB accumulation or laser irradiation causes a shift in the redox status of CECs towards increased reducing activity, without causing any cell damage. However, the combined action of PS and laser radiation has the opposite effect on the redox status of cells, resulting in an increase in the oxidized form of FAD.
dc.identifier.citationEffect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro / Makarov, V.I. [et al.] // Photonics. - 2024. - 11. - № 4. - 10.3390/photonics11040316
dc.identifier.doi10.3390/photonics11040316
dc.identifier.urihttps://www.doi.org/10.3390/photonics11040316
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85191688793&origin=resultslist
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/25988
dc.relation.ispartofPhotonics
dc.subjectMethylene blue
dc.subjectMTT assay
dc.titleEffect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue4
oaire.citation.volume11
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relation.isAuthorOfPublication93d3ea7f-0630-4969-ba26-3c921c1a44c4
relation.isAuthorOfPublication371640a5-5007-444c-a657-ccfac33ccebd
relation.isAuthorOfPublication.latestForDiscovery0b95f306-aed6-463c-a505-7039317b1493
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