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Three-finger proteins from snakes and humans acting on nicotinic receptors: Old and new

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dc.contributor.authorKasheverov, I. E.
dc.contributor.authorUtkin, Y. N.
dc.contributor.authorTsetlin, V. I.
dc.date.accessioned2024-11-29T10:20:31Z
dc.date.available2024-11-29T10:20:31Z
dc.date.issued2021
dc.description.abstract© 2020 International Society for NeurochemistryThe first toxin to give rise to the three-finger protein (TFP) family was α-bungarotoxin (α-Bgt) from Bungarus multicinctus krait venom. α-Bgt was crucial for research on nicotinic acetylcholine receptors (nAChRs), and in this Review article we focus on present data for snake venom TFPs and those of the Ly6/uPAR family from mammalians (membrane-bound Lynx1 and secreted SLURP-1) interacting with nAChRs. Recently isolated from Bungarus candidus venom, αδ-bungarotoxins differ from α-Bgt: they bind more reversibly and distinguish two binding sites in Torpedo californica nAChR. Naja kaouthia α-cobratoxin, classical blocker of nAChRs, was shown to inhibit certain GABA-A receptor subtypes, whereas α-cobratoxin dimer with 2 intermolecular disulfides has a novel type of 3D structure. Non-conventional toxin WTX has additional 5th disulfide not in the central loop, as α-Bgt, but in the N-terminal loop, like all Ly6/uPAR proteins, and inhibits α7 and Torpedo nAChRs. A water-soluble form of Lynx1, ws-Lynx1, was expressed in E. coli, its 1H-NMR structure and binding to several nAChRs determined. For SLURP-1, similar information was obtained with its recombinant analogue rSLURP-1. A common feature of ws-Lynx1, rSLURP-1, and WTX is their activity against nAChRs and muscarinic acetylcholine receptors. Synthetic SLURP-1, identical to the natural protein, demonstrated some differences from rSLURP-1 in distinguishing nAChR subtypes. The loop II fragment of the Lynx1 was synthesized having the same µM affinity for the Torpedo nAChR as ws-Lynx1. This review illustrates the productivity of parallel research of nAChR interactions with the two TFP groups. (Figure presented.).
dc.identifier.citationKasheverov, I. E. Three-finger proteins from snakes and humans acting on nicotinic receptors: Old and new / Kasheverov, I.E., Utkin, Y.N., Tsetlin, V.I. // Journal of Neurochemistry. - 2021. - 10.1111/jnc.15123
dc.identifier.doi10.1111/jnc.15123
dc.identifier.urihttps://www.doi.org/10.1111/jnc.15123
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85088450632&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000555519900001
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/23430
dc.relation.ispartofJournal of Neurochemistry
dc.titleThree-finger proteins from snakes and humans acting on nicotinic receptors: Old and new
dc.typeReview
dspace.entity.typePublication
relation.isOrgUnitOfPublicationc8407a6f-7272-450d-8d99-032352c76b55
relation.isOrgUnitOfPublication.latestForDiscoveryc8407a6f-7272-450d-8d99-032352c76b55
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