Персона:
Олейников, Владимир Александрович

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

Фамилия

Олейников

Имя

Владимир Александрович

Полная страница элемента

Результаты поиска

Теперь показываю 1 - 10 из 24

Только метаданные
Nanohybrid Structures Based on Plasmonic or Fluorescent Nanoparticles and Retinal-Containing Proteins
(2020) Zaitsev, S. Y.; Oleinikov, V. A.; Solovyeva, D. O.; Олейников, Владимир Александрович
© 2020, Pleiades Publishing, Ltd.Rhodopsins are light-sensitive membrane proteins enabling transmembrane charge separation (proton pump) on absorption of a light quantum. Bacteriorhodopsin (BR) is a transmembrane protein from halophilic bacteria that belongs to the rhodopsin family. Potential applications of BR are considered so promising that the number of studies devoted to the use of BR itself, its mutant variants, as well as hybrid materials containing BR, in various areas grows steadily. Formation of hybrid structures combining BR with nanoparticles is an essential step in promotion of BR-based devices. However, rapid progress, continuous emergence of new data, as well as challenges of analyzing the entire data require regular reviews of the achievements in this area. This review is devoted to the issues of formation of materials based on hybrids of BR with fluorescent semiconductor nanocrystals (quantum dots) and with noble metal (silver, gold) plasmonic nanoparticles. Recent data on formation of thin (mono-) and thick (multi-) layers from materials containing BR and BR/nanoparticle hybrids are presented.
Только метаданные
Quasistatic hysteresis loops of magnetic nanoparticles in a rotating magnetic field
(2020) Usov, N. A.; Gubanova, E. M.; Epstein, N. B.; Belyaeva, G. A.; Oleinikov, V. A.; Усов, Николай Александрович; Губанова, Елизавета Михайловна; Эпштейн, Наталья Борисовна; Олейников, Владимир Александрович
© 2019Quasistatic hysteresis loops of a single-domain magnetic nanoparticle with uniaxial anisotropy in a rotating magnetic field have been calculated. Magnetic hysteresis is shown to exist only in a limited range of reduced magnetic field amplitudes, 0.5 < h0 < 1, where h0 = H0/Ha, H0 is the amplitude of the rotating magnetic field, Ha being the particle anisotropy field. An analytical formula is obtained for the particle coercive force as a function of the reduced field amplitude. In the domain h0 < 0.5 the magnetization reversal of a particle is impossible, since the final energy barrier exists between the potential wells of the particle for all orientations of applied magnetic field. On the other hand, in the domain h0 > 1 the total energy of the nanoparticle has a single energy minimum, so that there is no magnetic hysteresis. Quasistatic hysteresis loops of a randomly oriented assembly of non interacting nanoparticles in a rotating magnetic field are also obtained.
Только метаданные
Interaction of SiFe Nanoparticles with Epithelial and Lymphoid Cells
(2020) Sharonova, N. V.; Svirshchevskaya, E. V.; Popov, A. A.; Karpov, N. V.; Tikhonovsky, G. V.; Zakharkiv, A. Y.; Timoshenko, V. Y.; Klimentov, S. M.; Oleinikov, V. A.; Попов, Антон Александрович; Тихоновский, Глеб Валерьевич; Захаркив, Анастасия Юрьевна; Тимошенко, Виктор Юрьевич; Климентов, Сергей Михайлович; Олейников, Владимир Александрович
Silicon and silicon-based nanoparticles (SiNP) attract scientific attention due to the biocompatibility and assimilation of silicon by body tissues. Iron-doped SiNP (SiFeNP) allow the use of ferromagnetic properties of iron for NP detection and the possibility of therapeutic application of SiFeNP. The purpose of this work was to analyze the interaction of SiFeNP with epithelial cells (EC) COLO357 and SW620 and human peripheral blood lymphocytes (PBL). SiFeNP were obtained by laser ablation and divided into the NP1 and NP2 fractions of 100 and 150 nm size, respectively. Cytotoxicity, apoptosis induction, reactive oxygen species (ROS) production, and lysosome metabolism were analyzed using in vitro methods. EC were found to efficiently incytosed both types of NPs, which resulted in the increase in the granularity of cells. NP did not cause apoptosis or EC necrosis, but accumulated in lysosomes, which led to a decrease in the membrane potential of lysosomes. In turn, a decrease in the level of EC metabolism led to a gradual (24 h) increase in ROS production by 10-15%. NP1 caused more ROS than NP2, and accumulated more in the EC, which may be the result of a difference in the particle size. SiFeNP did not interact with PBL. Thus, the total cytotoxicity of SiFeNP did not exceed 20%, which is associated with a decrease in lysosome metabolism and insignificant ROS production.
Только метаданные
Energy transfer mechanisms in nanobiohybrid structures based on quantum dots and photosensitive membrane proteins
(2016) Sizova, S. V.; Oleinikov, V. A.; Bouchonville, N.; Molinari, M.; Samokhvalov, P. S.; Sukhanova, A.; Nabiev, I.; Суханова, Алена Владимировна; Набиев, Игорь Руфаилович; Олейников, Владимир Александрович
The integration of novel nanomaterials with highly functional biological molecules has numerous advanced applications, including optoelectronics, biosensing, imaging, and energy harvesting. This review summarizes recent progress in understanding the mechanisms of energy transfer between semiconductor nanocrystal (so-called quantum dots [QDs]) and photosensitive proteins in heterostructures, such as hybrids of semiconductor nanocrystals with purple membranes containing bacteriorhodopsin (bR) or with photosynthetic reaction centers (RCs). Understanding of these mechanisms should enable prediction of the possible ways to improve the biological function of biomolecules by means of their assembling with QDs and develop novel functional materials with controlled photonic properties and applications. The possible mechanisms of energy transfer from QDs to photochromic biomolecules are discussed and correlated with experimental data. The principles of hybrid structures engineering, donor/acceptor parameters aﬀecting both energy transfer eﬃciency and biological function, and functionality of these hybrid structures are described. New nanobiohybrid materials are shown to have advanced implications for optoelectronics, photonics, and photovoltaics due to the ability of nanocomponents of these materials for eﬃcient energy harvesting, conversion, and transfer of additional energy to Biosystems, thus making them working more eﬃciently.
Только метаданные
Loading efficiency of doxorubicin into the micelle-like structures formed by function-spacer-lipid constructs self-assembly depends on constructs' functional part
(2021) Tarasova, A. R.; Vaskan, I. S.; Troitskaya, P. S.; Bovin, N. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Supramolecular self-assemble systems based on neoglycolipids: Galili-Ad-CMG2-Ad-DOPE, A(type2)-Ad-CMG2-Ad-DOPE are studied here and compared with the well-studied Biotin-CMG2-Ad-DOPE, as well as with their combinations with NH2-CMG2-Ad-DOPE. They are function-spacer-lipid constructs with unique structure that allows them to form micelle-like supramers and be stable, what makes them a potential drug nanocarriers. The structural properties of the obtained supramolecular systems are studied depending on their functional part, and the loading efficiency of doxorubicin into the supramers is determined to reveal the influence of the functional part. The resulting supramers were separated from the unbound molecules by dialysis, the nanoparticles morphology were studied by atomic force microscopy, and the loading efficiency was calculated based on spectrophotometry data. The encapsulation of doxorubicin was confirmed based on changes in the size and shape of the supramers, as well as a decrease in the ratio of unbound molecules. According to the loading efficiency calculations, it was estimated that supramers formed by A(type2)-Ad-CMG2-Ad-DOPE are the most efficient nanocarriers with loading efficiency of 82 %. Supramers formed by NH2-CMG2-Ad-DOPE (no functional part) showed 1.5 times less efficiency. Finally, the least efficient carriers are supramers formed by Biotin-CMG2-Ad-DOPE (14%).
Только метаданные
Small-angle X-ray (SAXS) and Raman spectroscopy studies of biot-CMG(2)-DOPE quasicrystalline phases
(2021) Maslennikov, A. M.; Shtykova, E. V.; Bovin, N. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Neoglycolipids due to their amphiphilic properties exhibit self-assembly in aqueous phases. In high concentrations the liquid crystalline or gel phases may form. So-called soft-material are a subject of interest of many scientists especially as biosensors and wound healing materials. In this study we examine the structure of a quasicrystalline phase of biot-CMG(2)DOPE obtained at the concentration of 150 mg/ml (13wt.%) in PBS. The structural data such as interplanar spacing, order parameter and long-range order were obtained by SAXS, while the changes in chemical structure were studied by Raman spectroscopy. It was also in our interest to examine a correlation between the ionic strength and the self-assembly, so we also studied a similar quasicrystalline phase of the same compound but in a buffer containing CaCl2 at the concentration of 4wt.%. According to SAXS data, FSL-biotin construct formed a complex ordered phase consisting of overlapping latices of different kind. The addition of CaCl2 into PBS resulted in obtaining a more structured system demonstrating cubic-like crystal lattice. Change in peak intensities on Raman spectrums of -C-H- and -C-C- bonds vibrations explained the change in phase properties.
Только метаданные
Comparative analysis of SERS-active colloidal silver solutions of various type and prospects of their applications
(2021) Altunina, A. V.; Zalygin, A. V.; Oleinikov, V. A.; Залыгин, Антон Владленович; Олейников, Владимир Александрович
© 2021 Institute of Physics Publishing. All rights reserved.Raman spectroscopy is a promising method for optical vibrational spectroscopy. Nowadays, Raman spectroscopy finds many applications, in particular, in biological and medical diagnostics. However, the Raman scattering can be enhanced using the Surface-enhanced Raman scattering method. Colloidal solutions of noble metals are used as SERS-active systems. In this work, the enhancing factors were estimated for colloidal silver solutions of three different types (citrate, borohydride, chloride) with two substances (phenylalanine, cytochrome C). Phenylalanine is a widely used model substance for Raman and Surface-enhanced Raman spectroscopy. Cytochrome C is one of the most researched proteins. It involves in the electron transport chain of the mitochondrial inner membrane and provides cellular respiration. Borohydride, citrate and chloride sols with phenylalanine gave an enhancement about 50, 200 and 30 times, respectively, and with cytochrome C about 30, 160 and 20, respectively. A comparative analysis of active and inactive sols by SERS and absorption spectroscopy was also performed. The absorption spectra of active sols have characteristic maxima in the region of 400 nm. Both the SERS method of model substances and absorption spectroscopy can be used to assess the enhancing properties.
Только метаданные
Investigation of the Distribution of Magnetic Nanoparticles in Tumor Tissues by the Method of Scanning Magnetic Force Nanotomography
(2022) Agapova, O. I.; Efimov, A. E.; Mochalov, K. E.; Solovyeva, D. O.; Klimentov, S. M.; Popov, A. A.; Oleinikov, V. A.; Климентов, Сергей Михайлович; Попов, Антон Александрович; Олейников, Владимир Александрович
© 2022, Pleiades Publishing, Ltd.Abstract: The development of effective biomedical technologies using magnetic nanoparticles (MNPs) for the tasks of oncotherapy and nanodiagnostics requires the development and implementation of new methods for the analysis of micro- and nanoscale distributions of MNPs in the volume of cells and tissues. The paper presents a new approach to three-dimensional analysis of MNP distributions - scanning magnetic force nanotomography as applied to the study of tumor tissues. Correlative reconstruction of MNP distributions and nanostructure features of the studied tissues made it possible to quantitatively estimate the parameters of three-dimensional distributions of composite nanoparticles based on silicon and iron oxide obtained by femtosecond laser ablation and injected intravenously and intratumorally into tumor tissue samples of B16/F1 mouse melanoma. The developed technology based on the principles of scanning probe nanotomography is applicable for studying the features of three-dimensional micro- and nanoscale distributions of magnetic nanoparticles in biomaterials, cells and tissues of various types.
Только метаданные
Modern Methods of Fluorescence Nanoscopy in Biology (A Review)
(2024) Solovyevа, D. O.; Altuninа, А. V.; Tretyak, M. V.; Mochalov, К. Е.; Oleinikov, V. A.; Олейников, Владимир Александрович
Только метаданные
Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation
(2022) Svirshchevskaya, E. V.; Sharonova, N. V.; Poltavtseva, R. A.; Konovalova, M. V.; Popov, A. A.; Oleinikov, V. A.; Попов, Антон Александрович; Олейников, Владимир Александрович
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.Multiple studies have demonstrated that various nanoparticles (NPs) stimulate osteo-genic differentiation of mesenchymal stem cells (MSCs) and inhibit adipogenic ones. The mechanisms of these effects are not determined. The aim of this paper was to estimate Wharton’s Jelly MSCs phenotype and humoral factor production during tri-lineage differentiation per se and in the presence of silicon–gold NPs. Silicon (SiNPs), gold (AuNPs), and 10% Au-doped Si nanoparti-cles (SiAuNPs) were synthesized by laser ablation, characterized, and studied in MSC cultures before and during differentiation. Humoral factor production (n = 41) was analyzed by Luminex technology. NPs were nontoxic, did not induce ROS production, and stimulated G-CSF, GM-CSF, VEGF, CXCL1 (GRO) production in four day MSC cultures. During MSC differentiation, all NPs stimulated CD13 and CD90 expression in osteogenic cultures. MSC differentiation resulted in a decrease in multiple humoral factor production to day 14 of incubation. NPs did not significantly affect the production in chondrogenic cultures and stimulated it in both osteogenic and adipogen-ic ones. The major difference in the protein production between osteogenic and adipogenic MSC cultures in the presence of NPs was VEGF level, which was unaffected in osteogenic cells and 4–9 times increased in adipogenic ones. The effects of NPs decreased in a row AuNPs > SiAuNPs > SiNPs. Taken collectively, high expression of CD13 and CD90 by MSCs and critical level of VEGF production can, at least, partially explain the stimulatory effect of NPs on MSC osteogenic differ-entiation.