Персона:
Поминова, Дарья Вячеславовна

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

Фамилия

Поминова

Имя

Дарья Вячеславовна

Полная страница элемента

Результаты поиска

Теперь показываю 1 - 10 из 18

Только метаданные
Fluorescence diagnostics and photodynamic therapy of grain crops pathogenic fungi
(2020) Bikmukhametova, I. R.; Akhlyustina, E. V.; Pominova, D. V.; Ryabova, A. V.; Grachev, P. V.; Makarov, V. I.; Kartabaeva, B. B.; Ахлюстина, Екатерина Витальевна; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Макаров, Владимир Игоревич
© 2020 IEEE.The studies show the dynamics of photosensitizers accumulation in various grain areas during germination and their photodynamic activity against pathogenic microflora (Fusarium, Bipolaris, Alternaria). Four photosensitizers (methylene blue, Chlorin E6, aluminum phthalocyanine in molecular- and nanoform) were used in the work. The accumulation level of methylene blue and aluminum phthalocyanine in molecular form in infected by Alternaria and Fusarium fungi grains was 4-5 times higher than in control on the 4th day. The possibility of pathogenic microflora inactivation using aluminum phthalocyanine was shown.
Только метаданные
PHOTO-INDUCED PROCESSES OF IRON OXIDE NANOPARTICLES TO ENHANCE LASER THERAPY фОТОИНдУЦИРОвАННЫЕ пРОЦЕССЫ НАНОчАСТИЦ ОКСИдА ЖЕЛЕЗА дЛя УСИЛЕНИя ЛАЗЕРНОи ТЕРАпИИ
(2021) Romanishkin, I. D.; Plotnikova, E. A.; Morozova, N. B.; Wittig, R.; Pominova, D. V.; Loschenov, V. B.; Steiner, R. W.; Ryabova, A. V.; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович; Штайнер, Рудольф Вольфганг; Рябова, Анастасия Владимировна
© 2021 Russian Photodynamic Association. All rights reserved.Nanoparticles are used as drug carriers to increase the selectivity and effectiveness of therapy, as well as for combined therapy that utilizes different effects. Iron oxide nanoparticles are promising in this aspect. Due to magnetic properties, they can be used as a contrast agent for magnetic resonance imaging. Also, iron oxide nanoparticles could be coated with a photosensitizer for photodynamic therapy and their laser or magnetic heating can be used for phototherapy. Local enhancement of the electromagnetic field near iron oxide nanoparticles can increase the fluorescence intensity of photosensitizers and the efficiency of singlet oxygen generation. This paper presents the results of a study of iron oxide nanoparticles focused on the photophysical aspects of the formation of “hot spots” under laser irradiation. The photoinduced effects of iron oxide nanoparticles observed in in vitro experiments lead to the rupture of lysosomes. Theoretical modeling showed that the heating of iron oxide nanoparticles with a radius of 35 nm under the action of laser radiation is about 89°C and 19°C for wavelengths of 458 and 561 nm, respectively. Local field enhancement occurs in pairs of nanoparticles of various sizes and strongly depends on the distance between them. The maximum gain is achieved at small distances between nanoparticles. For a dimer of nanoparticles with radii of 10 and 35 nm at a distance of 1 nm, an enhancement factor of two orders of magnitude was obtained. The investigated phenomenon of «hot spots» is in demand for precision therapy, because the photo-induced processes occur at small distances between nanoparticles, in areas of their high accumulation.
Только метаданные
Photodynamic therapy with 5-ALA induced PpIX effect on macrophages polarization
(2022) Ryabova, A. V.; Pominova, D. V.; Skobeltcin, A. S.; Romanishkin, I. D.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор Борисович
In this work, we have carried out the assessment of changes in the polarization of macrophages in response to photodynamic treatment according to the changes in the fluorescence lifetime of respiratory chain enzymes. The analysis of the time-resolved fluorescence of metabolic signatures and PpIX makes it possible to determine the cell metabolism types. © 2022 IEEE.
Только метаданные
Modeling of local field enhancement and laser heating effects in iron oxide nanoparticles
(2022) Pominova, D. V.; Romanishkin, I. D.; Ryabova, A. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
In this work, we have carried out theoretical modeling of heating of iron oxide nanoparticles under the action of laser radiation, modeling of scattering and absorption of exciting laser radiation on iron oxide nanoparticles and their dimers, as well as local field enhancement near individual iron oxide nanoparticles and between two nanoparticles of different sizes forming a dimer. © 2022 IEEE.
Только метаданные
Synthesis and spectroscopic studies of nanostructures based on upconversion nanoparticles coated with a photosensitizer for photodynamic therapy with infrared excitation
(2022) Pominova, D. V.; Proydakova, V. Y.; Romanishkin, I. D.; Ryabova, A. V.; Kuznetsov, S. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
In this work, we studied the processes of energy transfer from upconversion nanoparticles to photosensitizer molecules. The possibility of photodynamic therapy with infrared excitation using synthesized nanostructures has been demonstrated. © 2022 IEEE.
Только метаданные
Theranostic Properties of Crystalline Aluminum Phthalocyanine Nanoparticles as a Photosensitizer
(2022) Makarov, V. I.; Pominova, D. V.; Ryabova, A. V.; Romanishkin, I. D.; Voitova, A. V.; Steiner, R. W.; Loschenov, V. B.; Макаров, Владимир Игоревич; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
The study of phthalocyanines, known photosensitizers, for biomedical applications has been of high research interest for several decades. Of specific interest, nanophotosensitizers are crystalline aluminum phthalocyanine nanoparticles (AlPc NPs). In crystalline form, they are water-insoluble and atoxic, but upon contact with tumors, immune cells, or pathogenic microflora, they change their spectroscopic properties (acquire the ability to fluoresce and become phototoxic), which makes them upcoming agents for selective phototheranostics. Aqueous colloids of crystalline AlPc NPs with a hydrodynamic size of 104 В± 54 nm were obtained using ultrasonic dispersal and centrifugation. Intracellular accumulation and localization of AlPc were studied on HeLa and THP-1 cell cultures and macrophages (M0, M1, M2) by fluorescence microscopy. Crystallinity was assessed by XRD spectroscopy. Time-resolved spectroscopy was used to obtain characteristic fluorescence kinetics of AlPc NPs upon interaction with cell cultures. The photodynamic efficiency and fluorescence quantum yield of AlPc NPs in HeLa and THP-1 cells were evaluated. After entering the cells, AlPc NPs localized in lysosomes and fluorescence corresponding to individual AlPc molecules were observed, as well as destruction of lysosomes and a rapid decrease in fluorescence intensity during photodynamic action. The photodynamic efficiency of AlPc NPs in THP-1 cells was almost 1.8-fold that of the molecular form of AlPc (Photosens). A new mechanism for the occurrence of fluorescence and phototoxicity of AlPc NPs in interaction with cells is proposed.
Только метаданные
Synthesis Strategy of Tetrapyrrolic Photosensitizers for Their Practical Application in Photodynamic Therapy
(2022) Koifman, O. I.; Ageeva, T. A.; Kuzmina, N. S.; Ryabova, A. V.; Pominova, D. V.; Makarov, V. I.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Макаров, Владимир Игоревич; Лощенов, Виктор Борисович
Только метаданные
Study of energy transfer processes between the rare earth ions in tri-doped upconversion nanoparticles NaGdF4:Yb-Er-Tm and NaGdF4:Yb-Er-Ho
(2022) Proydakova, V. Yu.; Romanishkin, I. D.; Kuznetsov, S. V.; Pominova, D. V.; Grachev, P. V.; Ryabova, A. V.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна
© 2022 SPIE.Study of energy transfer processes between the rare earth ions in NaGdF4 nanoparticles tri-doped with rare earth ions Yb3+, Er3+ and Ho3+ or Tm3+ was carried out. The luminescence spectra in short-wave infrared and visible ranges were investigated. It was shown that Er3+ → Ho3+ energy transfer leads to Ho3+ luminescence increase. Both Er3+ and Ho3+ luminescence peaks were observed in short-wave infrared range. For Er3+ and Tm3+ co-doped nanoparticles it was hard to separate luminescence peaks in SWIR. However, both Er3+ and Tm3+ luminescence peaks were observed in visible range. We attribute this effects to Tm3+ → Er3+ energy transfer which occurs due to overlap of Er3+ and Tm3+ luminescence bands in short-wave infrared range which leads to Tm3+ luminescence decrease. This hypothesis was confirmed by study of β-NaGdF4 tri-doped with Yb3+, Er3+ and Tm3+ luminescence spectra during heating. The intensity of Tm3+ luminescence increased during heating due to non-resonant nature of Yb3+ →Tm3+ energy transfer and the shape of spectra changed.
Только метаданные
Metabolic stress of tumor microenvironment during photothermal therapy: Activation of an immune antitumor response
(2024) Ryabova, A. V.; Pominova, D. V.; Markova, I. V.; Loschenov, V. B.; Рябова, Анастасия Владимировна; Поминова, Дарья Вячеславовна; Лощенов, Виктор Борисович
Только метаданные
PHOTODYNAMIC THERAPY WITH METHYLENE BLUE AND CHLORIN e6 PHOTOSENSITIZERS: STUDY ON EHRLICH CARCINOMA MICE MODEL ФОТОДИНАМИЧЕСКАЯ ТЕРАПИЯ С ФОТОСЕНСИБИЛИЗАТОРАМИ МЕТИЛЕНОВЫИ СИНИИ И ХЛОРИН е6: ИССЛЕДОВАНИЕ НА МЫШИНОИ МОДЕЛИ КАРЦИНОМЫ ЭРЛИХА
(2024) Pominova, D. V.; Ryabova, A. V.; Skobeltsin, A. S.; Markova, I. V.; Romanishkin, I. D.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна
Hypoxia negatively affcts the effctiveness of all types of anticancer therapy, in particular photodynamic therapy (PDT). In this regard, various approaches to overcome the limitations associated with hypoxia are widely discussed in the literature, one of them is the use of photosensitizers (PS) operating through the fist mechanism of the photodynamic reaction, such as methylene blue (MB). Previously, we have demonstrated that MB can have a positive effect on tumor oxygenation. In this work, we investigated the photodynamic activity of MB and a combination of MB with chlorin e6 on a tumor in vivo using a model of Ehrlich carcinoma. PDT was studied with the joint and separate administration of chlorin e6 and MB. The accumulation and localization of MB and its combination with chlorin e6 in vivo was assessed using video ‹?uorescence and spectroscopic methods, and the effect of laser exposure on accumulation was analyzed. After the PDT with chlorin e6, MB and a combination of MB with chlorin e6, a good therapeutic effect and a decrease in the tumor growth rate were observed compared to the control, especially in groups with PDT with MB and with the simultaneous administration of chlorin e6 and MB. The level of tumor oxygenation on days 3 and 5 after PDT was higher for groups with irradiation, the highest oxygenation on the 5th day after PDT was observed in the group with PDT only with MB. Phasor diagrams of tumors after PDT show a deviation from the metabolic trajectory and a shift towards a longer lifetimes compared to the control tumor, which indicates the presence of lipid peroxidation products. Thus, tumor regression after PDT is associated with the direct destruction of tumor cells under the in‹?uence of reactive oxygen species formed during PDT. Thus, the effectiveness of PDT with the combined use of MB and chlorin e6 has been demonstrated, and the main mechanisms of the antitumor effect of the combination of these PS have been studied.