Персона: Ахлюстина, Екатерина Витальевна
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Екатерина Витальевна
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- ПубликацияОткрытый доступNew cationic photosensitizers: Photophysical properties and results of preliminary studies of antibacterial efficacy(2019) Tiganova, I. G.; Makarova, E. A.; Philipova, N. I.; Romanishkin, I. D.; Akhlyustina, E. V.; Meerovich, G. A.; Loschenov, V. B.; Ахлюстина, Екатерина Витальевна; Меерович, Геннадий Александрович; Лощенов, Виктор Борисович© 2019 Published under licence by IOP Publishing Ltd. Polycationic derivatives of synthetic bacteriochlorin with absorption in the near infrared range are promising for the creation of photosensitizers (PS) for antimicrobial photodynamic therapy. In the present work, the photophysical and antibacterial properties of PS based on tetracationic derivatives of synthetic bacteriochlorins: hydrophilic 3-Py 4 BC(EtBr) 4 Br4 in aqueous solution and amphiphilic 3-Py 4 BCHP 4 Br 4 in the dispersion of Kolliphor ELP were studied. Analysis of absorption and fluorescence in a wide range of concentrations has demonstrated low aggregation of the PS over the entire range. A high efficiency of photodynamic inactivation of Gram-positive Staphylococcus aureus and Gramnegative Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii bacteria was observed.
- ПубликацияТолько метаданныеMechanisms of the photodynamic effect with polycationic photosensitizers on the foci of bacterial and oncological diseases(2024) Meerovich, G. A.; Akhlyustina, E. V.; Makarova, E. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
- ПубликацияОткрытый доступPhotodynamic inactivation of Pseudomonas aeruginosa bacterial biofilms using photosensitizers based on octacationic derivatives of phthalocyanines and bacteriochlorins(2020) Tiganova, I. G.; Makarova, E. A.; Tolordava, E. R.; Zhizhimova, Yu. S.; Meerovich, G. A.; Akhlyustina, E. V.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович© Published under licence by IOP Publishing Ltd.Antibacterial photodynamic therapy is a promising method of treating local infected foci, especially surgical and burn wounds, trophic and diabetic ulcers. This work explores the photophysical and antibacterial properties of novel phthalocyanine- and synthetic-bacteriochlorin-based octacationic photosensitizers (PS). The results of the study confirm their low degree of aggregation at high concentrations, as well as high efficiency of photodynamic treatment of Gram-negative bacterial biofilms.
- ПубликацияТолько метаданныеNovel polycationic photosensitizers for antibacterial photodynamic therapy(2020) Tiganova, I. G.; Lukyanets, E. A.; Makarova, E. A.; Tolordava, E. R.; Meerovich, G. A.; Akhlyustina, E. V.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович© Springer Nature Switzerland AG 2019.Antibacterial photodynamic therapy (APDT) is a promising method of treating local infected foci, in particular, surgical and burn wounds, trophic and diabetic ulcers. Photodynamic inactivation (PDI) is able to effectively destroy bacterial cells without them developing resistance in response to treatment. This work was dedicated to the study of photophysical and antibacterial properties of new photosensitizers (PS) based on polycationic phthalocyanines and synthetic bacteriochlorins for photodynamic inactivation of P. aeruginosa bacteria and their biofilms. Gram-negative bacteria P. aeruginosa are often found in infected wounds, presumably in biofilm state and are characterized by rather low susceptibility to APDT, which is a problem. PS were studied for possible aggregation at various concentrations by means of absorption and fluorescence spectroscopy. The results of studies of the ZnPcChol8, (3-PyHp)4BCBr4 and (3-PyEBr)4BCBr4 in water and serum confirm the assumption of a low degree of their aggregation at high concentrations. Consequently, their photodynamic efficiency is high enabling to use these PS at high concentrations to sensitize pathological foci for APDT. It was shown that all the investigated PS had a high efficiency of photodynamic inactivation of Gram-negative bacteria P. aeruginosa, as well as their biofilms. Tetracationic hydrophilic near-infrared photosensitizer (3-PyEBr)4BCBr4 with reduced molecule size had significantly higher efficacy of photodynamic inactivation of P. aeruginosa biofilms compared with other studied photosensitizers.
- ПубликацияТолько метаданныеPhotodynamic inactivation of Pseudomonas aeruginosa bacterial biofilms using new polycationic photosensitizers(2019) Tiganova, I. G.; Lukyanets, E. A.; Makarova, E. A.; Tolordava, E. R.; Meerovich, G. A.; Akhlyustina, E. V.; Gonchukov, S. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Гончуков, Сергей Александрович; Лощенов, Виктор Борисович© 2019 Astro Ltd.The treatment of infected, long-term, non-healing, complicated wounds of the skin and mucosa, trophic ulcers, pressure sores and ulcers of diabetic feet represents a serious problem, especially in the case of resistant and multi-resistant pathogens. Antibacterial photodynamic therapy can be a promising method of local infected foci treatment of such diseases. This work is devoted to the study of the possibility of photodynamic inactivation (PDI) of Pseudomonas aeruginosa bacteria in the form of biofilms with the help of new polycationic photosensitizers (PSs) based on octacationic phthalocyanine and tetra-and octabacteriochlorins (ZnPcChol8, (3-PyBrE)4BCBr4 and (3-PyEPy)4BCBr8). More specifically, this work aims to clarify the role of light irradiation in PDI with the participation of new PSs, which has not been fully studied, especially with respect to the comparison of different PS types.
- ПубликацияТолько метаданныеOn the mechanisms of photodynamic action of photosensitizers based on polycationic derivatives of synthetic bacteriochlorin against human lung cancer cells A549 (in vitro study)(2022) Kogan, E. A.; Meerovich, G. A.; Karshieva, S. S.; Makarova, E. A.; Romanishkin, I. D.; Akhlyustina, E. V.; Meerovich, I. G.; Zharkov, N. V.; Demura, T. A.; Chen, Z. -L.; Koudan, E. V.; Angelov, I. P.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина ВитальевнаBackground: One of the tasks of anticancer photodynamic therapy is increasing the efficacy of treatment of cancer nodes with large (clinically relevant) sizes using near-infrared photosensitizers (PS). We study the photodynamic action against A549 human lung cancer cells using PS based on polycationic derivatives of synthetic bacteriochlorin. Methods: The efficacy and mechanisms of the photodynamic action of PS based on polycationic derivatives of synthetic bacteriochlorin against A549 lung cancer cells were studied in vitro using immunocytochemical and morphological methods. Results: It was found that PS based on tetracationic and octacationic derivatives of synthetic bacteriochlorin induce necrosis, apoptosis, decreasing of proliferative and mitotic activity, as well as reducing the number of ALDH1-positive cancer cells with signs of stem cells in A549 human lung cancer cell culture. The IC50 values (concentration of a PS that reduces cells survival by 50%) were about 0.69 μM for tetracationic PS and 0.57 μM for octacationic PS under irradiation at 30 J/cm2 while in the “dark” control they were higher than 100 μM for both PSs. Conclusions: Photosensitizers based on polycationic derivatives of synthetic bacteriochlorin have high phototoxicity against A549 cancer cells caused by the induction of necrosis and apoptosis of cancer cells, including cells with signs of stemness, and a sharp decrease of mitotic and proliferative activity. © 2022
- ПубликацияТолько метаданныеPhotodynamic action of photosensitizers based on polycationic derivatives of synthetic bacteriochlorin against human lung cancer cells A549(2022) Kogan, E. A.; Karshieva, S. S.; Makarova, E. A.; Dalina, A. A.; Meerovich, G. A.; Akhlyustina, E. V.; Gonchukov, S. A.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Гончуков, Сергей Александрович; Лощенов, Виктор Борисович© 2022 Astro Ltd.In view of their potential in the treatment of oncological diseases, photosensitizers (PS) have been the object of active research for the last years. The applied therapeutic photodynamic method involves the activation of a PS by light at a specific wavelength. PS interacts with oxygen and catalyzes the production of singlet oxygen molecules or radical oxygen species which leads to tumor cell death. This work is devoted to the study of the effectiveness of the photodynamic action of new PSs based on polycationic derivatives of synthetic bacteriochlorin on human lung cancer cells A549. The results obtained show that these PS effectively bind to these cells, have high phototoxicity and low 'dark' cytotoxicity for them. Morphological and immunohistochemical studies show that photodynamic effect leads to induction of cell necrosis and apoptosis, as well as a sharp decrease in mitotic activity.