Персона: Тихоновский, Глеб Валерьевич
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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Глеб Валерьевич
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- ПубликацияТолько метаданныеUltrafast laser ablation of gold in liquids: Effect of laser pulse overlap-induced surface porosity on size distribution of formed nanoparticles(2024) Ivanov, D. S.; Shakhov, P.; Tikhonowsky, G.; Popov, A. A.; Mayorov, A. N.; Zavestovskaya, I. N.; Klimentov, S. M.; Шахов, Павел Владимирович; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Майоров, Алексей Николаевич; Завестовская, Ирина Николаевна; Климентов, Сергей Михайлович
- ПубликацияОткрытый доступLaser-Synthesized Elemental Boron Nanoparticles for Efficient Boron Neutron Capture Therapy(2023) Zavestovskaya, I. N.; Zavestovskaya,I.N.; Babkova, J. S.; Zelepukin, I. V.; Tikhonowski, G. V.; Popov, A. A.; Klimentov, S. M.; Завестовская, Ирина Николаевна; Бабкова, Юлия Сергеевна; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Климентов, Сергей Михайлович; Деев, Сергей МихайловичBoron neutron capture therapy (BNCT) is one of the most appealing radiotherapy modalities, whose localization can be further improved by the employment of boron-containing nanoformulations, but the fabrication of biologically friendly, water-dispersible nanoparticles (NPs) with high boron content and favorable physicochemical characteristics still presents a great challenge. Here, we explore the use of elemental boron (B) NPs (BNPs) fabricated using the methods of pulsed laser ablation in liquids as sensitizers of BNCT. Depending on the conditions of laser-ablative synthesis, the used NPs were amorphous (a-BNPs) or partially crystallized (pc-BNPs) with a mean size of 20 nm or 50 nm, respectively. Both types of BNPs were functionalized with polyethylene glycol polymer to improve colloidal stability and biocompatibility. The NPs did not initiate any toxicity effects up to concentrations of 500 Вµg/mL, based on the results of MTT and clonogenic assay tests. The cells with BNPs incubated at a 10B concentration of 40 Вµg/mL were then irradiated with a thermal neutron beam for 30 min. We found that the presence of BNPs led to a radical enhancement in cancer cell death, namely a drop in colony forming capacity of SW-620 cells down to 12.6% and 1.6% for a-BNPs and pc-BNPs, respectively, while the relevant colony-forming capacity for U87 cells dropped down to 17%. The effect of cell irradiation by neutron beam uniquely was negligible under these conditions. Finally, to estimate the dose and regimes of irradiation for future BNCT in vivo tests, we studied the biodistribution of boron under intratumoral administration of BNPs in immunodeficient SCID mice and recorded excellent retention of boron in tumors. The obtained data unambiguously evidenced the effect of a neutron therapy enhancement, which can be attributed to efficient BNP-mediated generation of О±-particles.
- ПубликацияОткрытый доступLaser-Ablative Synthesis of Silicon–Iron Composite Nanoparticles for Theranostic Applications(2023) Bubnov, A. A.; Belov, V. S.; Kargina, Yu. V.; Tikhonowski, G. V.; Popov, A. A.; Kharin, A. Yu.; Shestakov, M. V.; Klimentov, S. M.; Timoshenko, V. Y.; Белов, Владимир Сергеевич; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Климентов, Сергей Михайлович; Тимошенко, Виктор ЮрьевичThe combination of photothermal and magnetic functionalities in one biocompatible nanoformulation forms an attractive basis for developing multifunctional agents for biomedical theranostics. Here, we report the fabrication of silicon-iron (Si-Fe) composite nanoparticles (NPs) for theranostic applications by using a method of femtosecond laser ablation in acetone from a mixed target combining silicon and iron. The NPs were then transferred to water for subsequent biological use. From structural analyses, it was shown that the formed Si-Fe NPs have a spherical shape and sizes ranging from 5 to 150 nm, with the presence of two characteristic maxima around 20 nm and 90 nm in the size distribution. They are mostly composed of silicon with the presence of a significant iron silicide content and iron oxide inclusions. Our studies also show that the NPs exhibit magnetic properties due to the presence of iron ions in their composition, which makes the formation of contrast in magnetic resonance imaging (MRI) possible, as it is verified by magnetic resonance relaxometry at the proton resonance frequency. In addition, the Si-Fe NPs are characterized by strong optical absorption in the window of relative transparency of bio-tissue (650-950 nm). Benefiting from such absorption, the Si-Fe NPs provide strong photoheating in their aqueous suspensions under continuous wave laser excitation at 808 nm. The NP-induced photoheating is described by a photothermal conversion efficiency of 33-42%, which is approximately 3.0-3.3 times larger than that for pure laser-synthesized Si NPs, and it is explained by the presence of iron silicide in the NP composition. Combining the strong photothermal effect and MRI functionality, the synthesized Si-Fe NPs promise a major advancement of modalities for cancer theranostics, including MRI-guided photothermal therapy and surgery.
- ПубликацияТолько метаданныеTunable optical properties of transition metal dichalcogenide nanoparticles synthesized by femtosecond laser ablation and fragmentation(2023) Chernikov, A. S.; Tselikov, G. I.; Gubin, M. Yu.; Romanov, R. I.; Popov, A. A.; Tikhonowski, G. V.; Tselikov, D.; Kabashin, A. V.; Романов, Роман Иванович; Попов, Антон Александрович; Тихоновский, Глеб Валерьевич; Целиков, Даниил Игоревич; Кабашин, Андрей ВикторовичControl of the chemical composition and optical properties of molybdenum disulfide nanoparticles by laser ablation and fragmentation is demonstrated.