Персона: Тихоновский, Глеб Валерьевич
Загружается...
Email Address
Birth Date
Научные группы
Научная группа
Организационные подразделения
Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
Статус
Фамилия
Тихоновский
Имя
Глеб Валерьевич
Имя
4 results
Результаты поиска
Теперь показываю 1 - 4 из 4
- ПубликацияОткрытый доступLaser-Synthesized Germanium Nanoparticles as Biodegradable Material for Near-Infrared Photoacoustic Imaging and Cancer Phototherapy(2024) Belyaev, I. B.; Zelepukin, I. V.; Kotelnikova, P. A.; Tikhonowski, G. V.; Popov, A. A.; Kopylov, A. N.; Deyev, S. M.; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Копылов, Алексей Николаевич; Деев, Сергей МихайловичAbstract Biodegradable nanomaterials can significantly improve the safety profile of nanomedicine. Germanium nanoparticles (Ge NPs) with a safe biodegradation pathway are developed as efficient photothermal converters for biomedical applications. Ge NPs synthesized by femtosecondў??laser ablation in liquids rapidly dissolve in physiologicalў??like environment through the oxidation mechanism. The biodegradation of Ge nanoparticles is preserved in tumor cells in vitro and in normal tissues in mice with a halfў??life as short as 3.5 days. Biocompatibility of Ge NPs is confirmed in vivo by hematological, biochemical, and histological analyses. Strong optical absorption of Ge in the nearў??infrared spectral range enables photothermal treatment of engrafted tumors in vivo, following intravenous injection of Ge NPs. The photothermal therapy results in a 3.9ў??fold reduction of the EMT6/P adenocarcinoma tumor growth with significant prolongation of the mice survival. Excellent massў??extinction of Ge NPs (7.9 L g ў??1 cm ў??1 at 808 nm) enables photoacoustic imaging of bones and tumors, following intravenous and intratumoral administrations of the nanomaterial. As such, strongly absorbing nearў??infraredў??light biodegradable Ge nanomaterial holds promise for advanced theranostics.
- ПубликацияОткрытый доступLaser-Ablative Synthesis of Silicon–Iron Composite Nanoparticles for Theranostic Applications(2023) Bubnov, A. A.; Belov, V. S.; Kargina, Yu. V.; Tikhonowski, G. V.; Popov, A. A.; Kharin, A. Yu.; Shestakov, M. V.; Klimentov, S. M.; Timoshenko, V. Y.; Белов, Владимир Сергеевич; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Климентов, Сергей Михайлович; Тимошенко, Виктор ЮрьевичThe combination of photothermal and magnetic functionalities in one biocompatible nanoformulation forms an attractive basis for developing multifunctional agents for biomedical theranostics. Here, we report the fabrication of silicon-iron (Si-Fe) composite nanoparticles (NPs) for theranostic applications by using a method of femtosecond laser ablation in acetone from a mixed target combining silicon and iron. The NPs were then transferred to water for subsequent biological use. From structural analyses, it was shown that the formed Si-Fe NPs have a spherical shape and sizes ranging from 5 to 150 nm, with the presence of two characteristic maxima around 20 nm and 90 nm in the size distribution. They are mostly composed of silicon with the presence of a significant iron silicide content and iron oxide inclusions. Our studies also show that the NPs exhibit magnetic properties due to the presence of iron ions in their composition, which makes the formation of contrast in magnetic resonance imaging (MRI) possible, as it is verified by magnetic resonance relaxometry at the proton resonance frequency. In addition, the Si-Fe NPs are characterized by strong optical absorption in the window of relative transparency of bio-tissue (650-950 nm). Benefiting from such absorption, the Si-Fe NPs provide strong photoheating in their aqueous suspensions under continuous wave laser excitation at 808 nm. The NP-induced photoheating is described by a photothermal conversion efficiency of 33-42%, which is approximately 3.0-3.3 times larger than that for pure laser-synthesized Si NPs, and it is explained by the presence of iron silicide in the NP composition. Combining the strong photothermal effect and MRI functionality, the synthesized Si-Fe NPs promise a major advancement of modalities for cancer theranostics, including MRI-guided photothermal therapy and surgery.
- ПубликацияОткрытый доступBoron Nanoparticle-Enhanced Proton Therapy for Cancer Treatment(2023) Zavestovskaya, I. N.; Tikhonowski, G. V.; Savinov, M. S.; Shakhov, P. V.; Babkova, J. S.; Popov, A. A.; Klimentov, S. M.; Prasad, P. N.; Deyev, S. M.; Завестовская, Ирина Николаевна; Тихоновский, Глеб Валерьевич; Савинов, Максим Сергеевич; Шахов, Павел Владимирович; Бабкова, Юлия Сергеевна; Попов, Антон Александрович; Климентов, Сергей Михайлович; Деев, Сергей МихайловичProton therapy is one of the promising radiotherapy modalities for the treatment of deep-seated and unresectable tumors, and its efficiency can further be enhanced by using boron-containing substances. Here, we explore the use of elemental boron (B) nanoparticles (NPs) as sensitizers for proton therapy enhancement. Prepared by methods of pulsed laser ablation in water, the used B NPs had a mean size of 50 nm, while a subsequent functionalization of the NPs by polyethylene glycol improved their colloidal stability in buffers. Laser-synthesized B NPs were efficiently absorbed by MNNG/Hos human osteosarcoma cells and did not demonstrate any remarkable toxicity effects up to concentrations of 100 ppm, as followed from the results of the MTT and clonogenic assay tests. Then, we assessed the efficiency of B NPs as sensitizers of cancer cell death under irradiation by a 160.5 MeV proton beam. The irradiation of MNNG/Hos cells at a dose of 3 Gy in the presence of 80 and 100 ppm of B NPs led to a 2- and 2.7-fold decrease in the number of formed cell colonies compared to control samples irradiated in the absence of NPs. The obtained data unambiguously evidenced the effect of a strong proton therapy enhancement mediated by B NPs. We also found that the proton beam irradiation of B NPs leads to the generation of reactive oxygen species (ROS), which evidences a possible involvement of the non-nuclear mechanism of cancer cell death related to oxidative stress. Offering a series of advantages, including a passive targeting option and the possibility of additional theranostic functionalities based on the intrinsic properties of B NPs (e.g., photothermal therapy or neutron boron capture therapy), the proposed concept promises a major advancement in proton beam-based cancer treatment.
- ПубликацияТолько метаданныеTunable optical properties of transition metal dichalcogenide nanoparticles synthesized by femtosecond laser ablation and fragmentation(2023) Chernikov, A. S.; Tselikov, G. I.; Gubin, M. Yu.; Romanov, R. I.; Popov, A. A.; Tikhonowski, G. V.; Tselikov, D.; Kabashin, A. V.; Романов, Роман Иванович; Попов, Антон Александрович; Тихоновский, Глеб Валерьевич; Целиков, Даниил Игоревич; Кабашин, Андрей ВикторовичControl of the chemical composition and optical properties of molybdenum disulfide nanoparticles by laser ablation and fragmentation is demonstrated.