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Калягина, Нина Анатольевна

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Калягина

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Нина Анатольевна

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Только метаданные
Spectroscopic Verification of Contrast Enhancement Methods in Fluorescence Diagnostics of Basal Cell Carcinoma with Scar Tissue
(2024) Febenchukova, A. A.; Udeneev, A. M.; Kulichenko, A. M.; Kalyagina, N. A.; Efendiev, K. T.; Loschenov, V. B.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович
Только метаданные
Dual channel video platform for fluorescence diagnostics in augmented reality
(2024) Loshchenov, M. V.; Udeneev, A. M.; Kalyagina, N. A.; Лощенов, Максим Викторович; Калягина, Нина Анатольевна
Открытый доступ
Photodynamic Therapy under Diagnostic Control of Wounds with Antibiotic-Resistant Microflora
(2024) Shiryaev, A.; Kalyagina, N.; Ivankov, M.; Efendiev, K.; Loschenov, V.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович
Background: Difficulties in the treatment of purulent wounds are caused by bacterial biofilms, which results in decontamination limitations. Infected wounds are not sufficiently susceptible to existing antibiotics, necessitating the search for alternative approaches to reduce the concentration of pathogenic microflora. Methods: This study describes an approach to the effective treatment of wounds by photodynamic inactivation or therapy (PDI/PDT) of antibiotic-resistant microflora under fluorescence control. For this purpose, laser and LED light (660ў??680 nm) and different groups of photosensitizers (PS) (1% solutions of methylene blue, aluminum phthalocyanine, chlorine e6 and nanocomposites containing these groups of PS) were used. The study included 90 patients with various wounds. Some patients were subjected to fluorescence diagnosis by laser spectral analysis before the PDT. Results: Positive results were achieved in 76 patients (84%, p andlt; 0.05). After the first PDT session, a decrease in the concentration of microflora was noticeable. By the third and seventh days, a significant to complete inactivation of bacteria was obtained. In all patients who were photo-diagnosed before PDT, a significant PS concentration decrease of more than 75% after PDT was obtained. Conclusion: PDT is an effective method for the inactivation of antibiotic-resistant pathogens, including in long non-healing wounds, contributing also to early tissue regeneration.
Только метаданные
Percutaneous nephrolithotomy and laparoscopic surgery efficacy and renal function outcomes for large and complex renal calculi
(2024) Pak,Y.; Kalyagina,N.; Yagudaev,D.; Калягина, Нина Анатольевна
Открытый доступ
Validation of a White Light and Fluorescence Augmented Panoramic Endoscopic Imaging System on a Bimodal Bladder Wall Experimental Model
(2024) Moskalev, A.; Kalyagina, N.; Kozlikina, E.; Loshchenov, M.; Калягина, Нина Анатольевна; Лощенов, Максим Викторович
Background: Fluorescence visualization of pathologies, primarily neoplasms in human internal cavities, is one of the most popular forms of diagnostics during endoscopic examination in medical practice. Currently, visualization can be performed in the augmented reality mode, which allows to observe areas of increased fluorescence directly on top of a usual color image. Another no less informative form of endoscopic visualization in the future can be mapping (creating a mosaic) of the acquired image sequence into a single map covering the area under study. The originality of the present contribution lies in the development of a new 3D bimodal experimental bladder model and its validation as an appropriate phantom for testing the combination of bimodal cystoscopy and image mosaicking. Methods: An original 3D real bladder-based phantom (physical model) including cancer-like fluorescent foci was developed and used to validate the combination of (i) a simultaneous white light and fluorescence cystoscopy imager with augmented reality mode and (ii) an image mosaicking algorithm superimposing both information. Results: Simultaneous registration and real-time visualization of a color image as a reference and a black-and-white fluorescence image with an overlay of the two images was made possible. The panoramic image build allowed to precisely visualize the relative location of the five fluorescent foci along the trajectory of the endoscope tip. Conclusions: The method has broad prospects and opportunities for further developments in bimodal endoscopy instrumentation and automatic image mosaicking.
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Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions
(2020) Vervald, A. M.; Vervald, E. N.; Burikov, S. A.; Patsaeva, S. V.; Kalyagina, N. A.; Калягина, Нина Анатольевна
Copyright © 2020 American Chemical Society.The interactions of one of the most famous enzymes, lysozyme, with carboxylated nanodiamonds in water were studied. It was found that stable complexes are formed as a result of lysozyme adsorption on the surface of nanodiamonds. Based on the obtained adsorption isotherms and change in the fluorescence of nanodiamonds during the adsorption of lysozyme on them, it is concluded that lysozyme is adsorbed on carboxylated nanodiamonds in two layers. Numerical estimates and IR absorption spectroscopy data showed that the lysozyme has different adsorption orientations in the first and second layers, with preferential side-on and end-on orientations, correspondingly. Moreover, in the first layer, lysozyme undergoes significant conformational changes. The enzymatic activity of adsorbed lysozyme in both layers is discussed.
Только метаданные
Laser-induced fluorescence diagnosis of stomach tumor
(2020) Loshchenov, M.; Levkin, V.; Linkov, K.; Kharnas, S.; Kalyagina, N.; Efendiev, K.; Loschenov, V.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович
© 2020, Springer-Verlag London Ltd., part of Springer Nature.The purpose of this study is to demonstrate the capabilities of laser spectral and video fluorescence diagnosis used for stomach tumors using 5-ALA photosensitizer. The spectroscopic method is presented with an example of a characteristic fluorescence spectrum from stomach with 5-ALA and quantitative statistics. The laser excitation wavelength was 632.8 nm. The analysis of the video system is presented with clinical statistics. The penetration depth of 3–4 mm of the He–Ne laser during the spectroscopic study allowed for scanning the mucous and submucous layers of the stomach and for detecting tumorous growths in these layers. Registration of fluorescence using the spectral system enabled surgeons to conduct express estimation of dubious stomach tissues, to make biopsy from doubtful areas to reveal precancer and early cancer states. The video fluorescence analysis with the application of 5-ALA-induced PPIX may be recommended for the use as an express method of diagnosis including early diagnosis of malignant stomach diseases as well as for intraoperative assessment of tumor extension and detection of canceromatous foci during laparoscopy. The optimal time interval for the diagnosis (regardless of the nature of the study – endoscopic, laparoscopic, or intraoperative) is 2–4 h from the administration of photosensitizer. The optimal dose of the photosensitizer is 20 mg per 1 kg of the body weight.
Только метаданные
Two diagnostic criteria of optical spectroscopy for bladder tumor detection: Clinical study using 5-ALA induced fluorescence and mathematical modeling
(2020) Loshchenov, M.; Amouroux, M.; Daul, C.; Kudashev, B.; Kalyagina, N.; Loschenov, V.; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович
© 2020 Elsevier B.V.Background: The study proposes to improve bladder cancer diagnosis by photodynamic diagnosis (PDD) using red-light excitation (632.8 nm) of 5-ALA induced-protoporphyrin IX. Employing 9 patients’ bladders, two types of signals were used to improve diagnostic accuracy for malignancy and we also present numerical modeling of the scattering coefficient to provide biological explanation of the results obtained. Methods: Two modalities of bladder cancer spectral diagnosis are presented: conventional PDD and intensity assessment of the diffusely reflected laser light by fiber-optic spectroscopy. Experiments are done in clinical conditions and as a series of numerical simulations. Results: High-grade cancerous bladder tissues display twice a higher relative fluorescence intensity (mean value 1, n = 9) than healthy (0.39, n = 9), dysplastic (0.44, n = 5) tissues and CIS (0.39, n = 2). The laser back-scattering signal allows to discriminate most effectively high-grade cancerous and dysplastic tissues from normal. Numerical modeling of diffuse reflectance spectra reveals that spectral behavior of the back-scattered light depends on both, nuclear size and nuclear density of tumoral cells. Conclusions: Unlike the fluorescence signal, where its value is higher in the case of pathological tissues, the tendency of the laser signal to, both, decrease or increase in comparison with the signal from normal urothelium, should be perceived as a sign towards neoplasm. Numerical simulation reveals that such a double-analysis at a multiwavelength mode potentially may be used to provide diagnostic accuracy.
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Optical biopsy: Fundamentals and applications in neurosurgery
(2021) Romanishkin, I. D.; Maklygina, Yu. S.; Borodkin, A. V.; Tsarukaev, B. A.; Savelieva, T. A.; Grachev, P. V.; Kalyagina, N. A.; Orlov, A. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович
© 2021 Institute of Physics Publishing. All rights reserved.Currently, there is a significant increase in the incidence of cancer of the central nervous system. Determination of the boundaries of intracerebral and intramedullary tumors is especially difficult. The urgency of the problem of determining the boundaries of astrocytic tumors is due to the peculiarities of their growth along myelinated nerve fibers and vessels, leading to the infiltration of healthy white matter by tumor cells, which affects the high frequency of postoperative relapses. The complexity of surgery for intramedullary tumors of the spinal cord is that the tumor does not always have a clear border and the risk of injury is high due to the smaller size of the operated area compared to the brain. Reliable information regarding the volume of the resected tumor should be obtained by intraoperative imaging. The solution to this problem is implemented mainly in three directions: the use of intraoperative computed tomography, magnetic resonance imaging and ultrasound scanning, and various combinations of these methods. Unfortunately, all these methods of intraoperative diagnostics do not allow real-time examination of tissues in an operating wound and/or do not provide a simultaneous analysis of both structural and metabolic changes. The limitations of intraoperative navigation methods in neurosurgery have led to the relevance of the development of an accurate spectroscopic method for in vivo determination of the content of specific metabolic markers and structural changes accompanying the development of the tumor process in the nervous tissue. Various approaches to intraoperative navigation based on optical spectroscopy are called optical biopsy. In this article, we present the methods and tools developed in recent years for spectroscopic guidance in neurooncology. First of all, this, of course, concerns the analysis of spectral dependences recorded before, during and after tumor removal. We have used such modalities of optical spectroscopy as fluorescence, diffuse reflectance spectroscopy and spontaneous Raman scattering. An equally important issue on the way to increasing the efficiency of tumor resection is the development of new instrumentation; therefore, we have developed a number of new devices, which are a combination of well-known neurosurgical instruments and laser and fiber-optic technologies. Last but not least is the issue of rapid classification of the studied tissues based on the recorded signals, which was solved by us using machine learning methods.
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Intraoperative video-fluorescence navigation by PpIX and tissue saturation measurement during surgical resection of gastric malignant tumor
(2022) Kustov, D. M.; Yakovlev, D. V.; Moskalev, A. S.; Kozlikina, E. I.; Loshchenov, M. V.; Kalyagina, N. A.; Loschenov, V. B.; Лощенов, Максим Викторович; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович
Fluorescence visualization of pathologies is a popular form of endoscopic diagnostics in medicine. Interest in fluorescence optical visualization consists in providing information on the fluorescent signal spatial distribution by the photosensitizer fluorescence, which has a selective accumulation in tumor. Together with this, the determination of tissue saturation makes possible to assess the adequate of blood supply to anastomosis during surgical operation. © 2022 IEEE.