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Калягина, Нина Анатольевна

Организационные подразделения

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Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

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Калягина

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Нина Анатольевна

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Только метаданные
Spectroscopic Verification of Contrast Enhancement Methods in Fluorescence Diagnostics of Basal Cell Carcinoma with Scar Tissue
(2024) Febenchukova, A. A.; Udeneev, A. M.; Kulichenko, A. M.; Kalyagina, N. A.; Efendiev, K. T.; Loschenov, V. B.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович
Только метаданные
Dual channel video platform for fluorescence diagnostics in augmented reality
(2024) Loshchenov, M. V.; Udeneev, A. M.; Kalyagina, N. A.; Лощенов, Максим Викторович; Калягина, Нина Анатольевна
Только метаданные
Bilayer Adsorption of Lysozyme on Nanodiamonds in Aqueous Suspensions
(2020) Vervald, A. M.; Vervald, E. N.; Burikov, S. A.; Patsaeva, S. V.; Kalyagina, N. A.; Калягина, Нина Анатольевна
Copyright © 2020 American Chemical Society.The interactions of one of the most famous enzymes, lysozyme, with carboxylated nanodiamonds in water were studied. It was found that stable complexes are formed as a result of lysozyme adsorption on the surface of nanodiamonds. Based on the obtained adsorption isotherms and change in the fluorescence of nanodiamonds during the adsorption of lysozyme on them, it is concluded that lysozyme is adsorbed on carboxylated nanodiamonds in two layers. Numerical estimates and IR absorption spectroscopy data showed that the lysozyme has different adsorption orientations in the first and second layers, with preferential side-on and end-on orientations, correspondingly. Moreover, in the first layer, lysozyme undergoes significant conformational changes. The enzymatic activity of adsorbed lysozyme in both layers is discussed.
Только метаданные
Optical biopsy: Fundamentals and applications in neurosurgery
(2021) Romanishkin, I. D.; Maklygina, Yu. S.; Borodkin, A. V.; Tsarukaev, B. A.; Savelieva, T. A.; Grachev, P. V.; Kalyagina, N. A.; Orlov, A. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович
© 2021 Institute of Physics Publishing. All rights reserved.Currently, there is a significant increase in the incidence of cancer of the central nervous system. Determination of the boundaries of intracerebral and intramedullary tumors is especially difficult. The urgency of the problem of determining the boundaries of astrocytic tumors is due to the peculiarities of their growth along myelinated nerve fibers and vessels, leading to the infiltration of healthy white matter by tumor cells, which affects the high frequency of postoperative relapses. The complexity of surgery for intramedullary tumors of the spinal cord is that the tumor does not always have a clear border and the risk of injury is high due to the smaller size of the operated area compared to the brain. Reliable information regarding the volume of the resected tumor should be obtained by intraoperative imaging. The solution to this problem is implemented mainly in three directions: the use of intraoperative computed tomography, magnetic resonance imaging and ultrasound scanning, and various combinations of these methods. Unfortunately, all these methods of intraoperative diagnostics do not allow real-time examination of tissues in an operating wound and/or do not provide a simultaneous analysis of both structural and metabolic changes. The limitations of intraoperative navigation methods in neurosurgery have led to the relevance of the development of an accurate spectroscopic method for in vivo determination of the content of specific metabolic markers and structural changes accompanying the development of the tumor process in the nervous tissue. Various approaches to intraoperative navigation based on optical spectroscopy are called optical biopsy. In this article, we present the methods and tools developed in recent years for spectroscopic guidance in neurooncology. First of all, this, of course, concerns the analysis of spectral dependences recorded before, during and after tumor removal. We have used such modalities of optical spectroscopy as fluorescence, diffuse reflectance spectroscopy and spontaneous Raman scattering. An equally important issue on the way to increasing the efficiency of tumor resection is the development of new instrumentation; therefore, we have developed a number of new devices, which are a combination of well-known neurosurgical instruments and laser and fiber-optic technologies. Last but not least is the issue of rapid classification of the studied tissues based on the recorded signals, which was solved by us using machine learning methods.
Только метаданные
Intraoperative video-fluorescence navigation by PpIX and tissue saturation measurement during surgical resection of gastric malignant tumor
(2022) Kustov, D. M.; Yakovlev, D. V.; Moskalev, A. S.; Kozlikina, E. I.; Loshchenov, M. V.; Kalyagina, N. A.; Loschenov, V. B.; Лощенов, Максим Викторович; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович
Fluorescence visualization of pathologies is a popular form of endoscopic diagnostics in medicine. Interest in fluorescence optical visualization consists in providing information on the fluorescent signal spatial distribution by the photosensitizer fluorescence, which has a selective accumulation in tumor. Together with this, the determination of tissue saturation makes possible to assess the adequate of blood supply to anastomosis during surgical operation. © 2022 IEEE.
Только метаданные
PHOTO AND SPECTRAL FLUORESCENCE ANALYSIS OF THE SPINAL CORD INJURY AREA IN ANIMAL MODELS ФОТО И СПЕКТРАЛЬНЫИ ФЛУОРЕСЦЕНТНЫИ АНАЛИЗ ОБЛАСТИ ТРАВМЫ СПИННОГО МОЗГА НА ЖИВОТНЫХ МОДЕЛЯХ
(2023) Udeneev, A. M.; Kalyagina, N. A.; Skobeltsin, A. S.; Loschenov, V. B.; Калягина, Нина Анатольевна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор Борисович
The purpose of the work is to follow the dynamics of changes in fluorescent signals in the near-surface layers of tissue of injured areas of the back of laboratory animals, which will allow, by indirect evidence, to evaluate the information content of fluorescence diagnosis for subsequent possible diagnostic monitoring of photodynamic therapy of the spinal cord. The model animals were Wistar rats. Two types of contusions were modeled: pneumo-contusion and contusion by a falling load. Methylene blue and indocyanine green were used as photosensitizers. Fluorescence measurements were carried out by imaging and spectrometric methods. A stroboscopic fluorescence imager with an excitation wavelength of 630 nm was used to acquire fluorescence images. The LESA-01-BIOSPEC spectrometer with a He-Ne laser excitation allowed to obtain spectra. It was shown that both methods make it possible to estimate the fluorescence value of methylene blue and indocyanine green in the tissues under study. Moreover, the photographic method also allows to obtain the spatial distribution of fluorescence. The general trend found in the data is a more intense and uniform fluorescence of the dorsal region of rats with methylene blue and a less intense, but more contrasting distribution of indocyanine green. The presented methods are non-invasive, which makes them attractive for diagnostic use. However, due to the shallow depth of signal reception, the condition of the spine can be determined only indirectly, by the condition of the near-surface layers of tissue that accumulate the photosensitizer.
Только метаданные
Cost-effective device for locating and circumscribing superficial tumors with contrast enhancement and fluorescence quantification
(2024) Udeneev, A. M.; Kalyagina, N. A.; Efendiev, K. T.; Febenchukova, A. A.; Kulichenko, A. M.; Loshchenov, M. V.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Максим Викторович
Two Bispectral contrast enhancement approaches for the fluorescence diagnosis with chlorine-e6 and a wide field-of-view imaging system with fluorescence excitation at 405 nm and time-resolved background suppression were analyzed and compared. Two techniques for the contrast enhancement of a fluorescent video system (Red/Green (R/G) ratio and Red-Green (R-G)) with time-resolved background suppression for fluorescent diagnosis (FD) were tested in four patients with basal cell carcinoma (BCC). The results of both contrast enhancement methods were compared for the diagnostic efficiency for FD of BCC. Both techniques successfully determined the boundaries of the lesions and the fluorescence intensity. Both contrast enhancement modes have proven effective in identifying tumor borders in cases of low contrast in BCC FD with Ce6. While the Red/Green (R/G) mode provides sharper lesion borders, the Red minus Green (R-G) mode visualizes more fluorescent features and makes it easier to assess the lesion margins.
Только метаданные
Comparison of Ce6 photobleaching rate on the surface and in the depth of basalioma during photodynamic therapy at a wavelength of 660 nm using registration of fluorescence excited in the red and violet ranges
(2024) Udeneev, A. M.; Febenchukova, A. A.; Kalyagina, N. A.; Калягина, Нина Анатольевна