Персона: Калягина, Нина Анатольевна
Загружается...
Email Address
Birth Date
Научные группы
Организационные подразделения
Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
Статус
Фамилия
Калягина
Имя
Нина Анатольевна
Имя
7 results
Результаты поиска
Теперь показываю 1 - 7 из 7
- ПубликацияТолько метаданныеSpectroscopic Verification of Contrast Enhancement Methods in Fluorescence Diagnostics of Basal Cell Carcinoma with Scar Tissue(2024) Febenchukova, A. A.; Udeneev, A. M.; Kulichenko, A. M.; Kalyagina, N. A.; Efendiev, K. T.; Loschenov, V. B.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович
- ПубликацияОткрытый доступPhotodynamic Therapy under Diagnostic Control of Wounds with Antibiotic-Resistant Microflora(2024) Shiryaev, A.; Kalyagina, N.; Ivankov, M.; Efendiev, K.; Loschenov, V.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор БорисовичBackground: Difficulties in the treatment of purulent wounds are caused by bacterial biofilms, which results in decontamination limitations. Infected wounds are not sufficiently susceptible to existing antibiotics, necessitating the search for alternative approaches to reduce the concentration of pathogenic microflora. Methods: This study describes an approach to the effective treatment of wounds by photodynamic inactivation or therapy (PDI/PDT) of antibiotic-resistant microflora under fluorescence control. For this purpose, laser and LED light (660ў??680 nm) and different groups of photosensitizers (PS) (1% solutions of methylene blue, aluminum phthalocyanine, chlorine e6 and nanocomposites containing these groups of PS) were used. The study included 90 patients with various wounds. Some patients were subjected to fluorescence diagnosis by laser spectral analysis before the PDT. Results: Positive results were achieved in 76 patients (84%, p andlt; 0.05). After the first PDT session, a decrease in the concentration of microflora was noticeable. By the third and seventh days, a significant to complete inactivation of bacteria was obtained. In all patients who were photo-diagnosed before PDT, a significant PS concentration decrease of more than 75% after PDT was obtained. Conclusion: PDT is an effective method for the inactivation of antibiotic-resistant pathogens, including in long non-healing wounds, contributing also to early tissue regeneration.
- ПубликацияТолько метаданныеLaser-induced fluorescence diagnosis of stomach tumor(2020) Loshchenov, M.; Levkin, V.; Linkov, K.; Kharnas, S.; Kalyagina, N.; Efendiev, K.; Loschenov, V.; Калягина, Нина Анатольевна; Эфендиев, Канамат Темботович; Лощенов, Виктор Борисович© 2020, Springer-Verlag London Ltd., part of Springer Nature.The purpose of this study is to demonstrate the capabilities of laser spectral and video fluorescence diagnosis used for stomach tumors using 5-ALA photosensitizer. The spectroscopic method is presented with an example of a characteristic fluorescence spectrum from stomach with 5-ALA and quantitative statistics. The laser excitation wavelength was 632.8 nm. The analysis of the video system is presented with clinical statistics. The penetration depth of 3–4 mm of the He–Ne laser during the spectroscopic study allowed for scanning the mucous and submucous layers of the stomach and for detecting tumorous growths in these layers. Registration of fluorescence using the spectral system enabled surgeons to conduct express estimation of dubious stomach tissues, to make biopsy from doubtful areas to reveal precancer and early cancer states. The video fluorescence analysis with the application of 5-ALA-induced PPIX may be recommended for the use as an express method of diagnosis including early diagnosis of malignant stomach diseases as well as for intraoperative assessment of tumor extension and detection of canceromatous foci during laparoscopy. The optimal time interval for the diagnosis (regardless of the nature of the study – endoscopic, laparoscopic, or intraoperative) is 2–4 h from the administration of photosensitizer. The optimal dose of the photosensitizer is 20 mg per 1 kg of the body weight.
- ПубликацияТолько метаданныеTwo diagnostic criteria of optical spectroscopy for bladder tumor detection: Clinical study using 5-ALA induced fluorescence and mathematical modeling(2020) Loshchenov, M.; Amouroux, M.; Daul, C.; Kudashev, B.; Kalyagina, N.; Loschenov, V.; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович© 2020 Elsevier B.V.Background: The study proposes to improve bladder cancer diagnosis by photodynamic diagnosis (PDD) using red-light excitation (632.8 nm) of 5-ALA induced-protoporphyrin IX. Employing 9 patients’ bladders, two types of signals were used to improve diagnostic accuracy for malignancy and we also present numerical modeling of the scattering coefficient to provide biological explanation of the results obtained. Methods: Two modalities of bladder cancer spectral diagnosis are presented: conventional PDD and intensity assessment of the diffusely reflected laser light by fiber-optic spectroscopy. Experiments are done in clinical conditions and as a series of numerical simulations. Results: High-grade cancerous bladder tissues display twice a higher relative fluorescence intensity (mean value 1, n = 9) than healthy (0.39, n = 9), dysplastic (0.44, n = 5) tissues and CIS (0.39, n = 2). The laser back-scattering signal allows to discriminate most effectively high-grade cancerous and dysplastic tissues from normal. Numerical modeling of diffuse reflectance spectra reveals that spectral behavior of the back-scattered light depends on both, nuclear size and nuclear density of tumoral cells. Conclusions: Unlike the fluorescence signal, where its value is higher in the case of pathological tissues, the tendency of the laser signal to, both, decrease or increase in comparison with the signal from normal urothelium, should be perceived as a sign towards neoplasm. Numerical simulation reveals that such a double-analysis at a multiwavelength mode potentially may be used to provide diagnostic accuracy.
- ПубликацияТолько метаданныеOptical biopsy: Fundamentals and applications in neurosurgery(2021) Romanishkin, I. D.; Maklygina, Yu. S.; Borodkin, A. V.; Tsarukaev, B. A.; Savelieva, T. A.; Grachev, P. V.; Kalyagina, N. A.; Orlov, A. V.; Loschenov, V. B.; Савельева, Татьяна Александровна; Калягина, Нина Анатольевна; Лощенов, Виктор Борисович© 2021 Institute of Physics Publishing. All rights reserved.Currently, there is a significant increase in the incidence of cancer of the central nervous system. Determination of the boundaries of intracerebral and intramedullary tumors is especially difficult. The urgency of the problem of determining the boundaries of astrocytic tumors is due to the peculiarities of their growth along myelinated nerve fibers and vessels, leading to the infiltration of healthy white matter by tumor cells, which affects the high frequency of postoperative relapses. The complexity of surgery for intramedullary tumors of the spinal cord is that the tumor does not always have a clear border and the risk of injury is high due to the smaller size of the operated area compared to the brain. Reliable information regarding the volume of the resected tumor should be obtained by intraoperative imaging. The solution to this problem is implemented mainly in three directions: the use of intraoperative computed tomography, magnetic resonance imaging and ultrasound scanning, and various combinations of these methods. Unfortunately, all these methods of intraoperative diagnostics do not allow real-time examination of tissues in an operating wound and/or do not provide a simultaneous analysis of both structural and metabolic changes. The limitations of intraoperative navigation methods in neurosurgery have led to the relevance of the development of an accurate spectroscopic method for in vivo determination of the content of specific metabolic markers and structural changes accompanying the development of the tumor process in the nervous tissue. Various approaches to intraoperative navigation based on optical spectroscopy are called optical biopsy. In this article, we present the methods and tools developed in recent years for spectroscopic guidance in neurooncology. First of all, this, of course, concerns the analysis of spectral dependences recorded before, during and after tumor removal. We have used such modalities of optical spectroscopy as fluorescence, diffuse reflectance spectroscopy and spontaneous Raman scattering. An equally important issue on the way to increasing the efficiency of tumor resection is the development of new instrumentation; therefore, we have developed a number of new devices, which are a combination of well-known neurosurgical instruments and laser and fiber-optic technologies. Last but not least is the issue of rapid classification of the studied tissues based on the recorded signals, which was solved by us using machine learning methods.
- ПубликацияТолько метаданныеIntraoperative video-fluorescence navigation by PpIX and tissue saturation measurement during surgical resection of gastric malignant tumor(2022) Kustov, D. M.; Yakovlev, D. V.; Moskalev, A. S.; Kozlikina, E. I.; Loshchenov, M. V.; Kalyagina, N. A.; Loschenov, V. B.; Лощенов, Максим Викторович; Калягина, Нина Анатольевна; Лощенов, Виктор БорисовичFluorescence visualization of pathologies is a popular form of endoscopic diagnostics in medicine. Interest in fluorescence optical visualization consists in providing information on the fluorescent signal spatial distribution by the photosensitizer fluorescence, which has a selective accumulation in tumor. Together with this, the determination of tissue saturation makes possible to assess the adequate of blood supply to anastomosis during surgical operation. © 2022 IEEE.
- ПубликацияТолько метаданныеPHOTO AND SPECTRAL FLUORESCENCE ANALYSIS OF THE SPINAL CORD INJURY AREA IN ANIMAL MODELS ФОТО И СПЕКТРАЛЬНЫИ ФЛУОРЕСЦЕНТНЫИ АНАЛИЗ ОБЛАСТИ ТРАВМЫ СПИННОГО МОЗГА НА ЖИВОТНЫХ МОДЕЛЯХ(2023) Udeneev, A. M.; Kalyagina, N. A.; Skobeltsin, A. S.; Loschenov, V. B.; Калягина, Нина Анатольевна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор БорисовичThe purpose of the work is to follow the dynamics of changes in fluorescent signals in the near-surface layers of tissue of injured areas of the back of laboratory animals, which will allow, by indirect evidence, to evaluate the information content of fluorescence diagnosis for subsequent possible diagnostic monitoring of photodynamic therapy of the spinal cord. The model animals were Wistar rats. Two types of contusions were modeled: pneumo-contusion and contusion by a falling load. Methylene blue and indocyanine green were used as photosensitizers. Fluorescence measurements were carried out by imaging and spectrometric methods. A stroboscopic fluorescence imager with an excitation wavelength of 630 nm was used to acquire fluorescence images. The LESA-01-BIOSPEC spectrometer with a He-Ne laser excitation allowed to obtain spectra. It was shown that both methods make it possible to estimate the fluorescence value of methylene blue and indocyanine green in the tissues under study. Moreover, the photographic method also allows to obtain the spatial distribution of fluorescence. The general trend found in the data is a more intense and uniform fluorescence of the dorsal region of rats with methylene blue and a less intense, but more contrasting distribution of indocyanine green. The presented methods are non-invasive, which makes them attractive for diagnostic use. However, due to the shallow depth of signal reception, the condition of the spine can be determined only indirectly, by the condition of the near-surface layers of tissue that accumulate the photosensitizer.