Персона: Лощенов, Виктор Борисович
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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- ПубликацияОткрытый доступEffect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro(2024) Makarov, V. I.; Skobeltsin, A. S.; Averchuk, A. S.; Loschenov, V. B.; Макаров, Владимир Игоревич; Скобельцин, Алексей Сергеевич; Лощенов, Виктор БорисовичBackground: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. Methods: The study was performed on the primary culture of CECs. The cells underwent photodynamic treatment through 660 nm laser irradiation at a power density of 300 mW/cm2. The MTT, TMRE, and TUNEL assays were used to assess the survival, redox metabolism, mitochondrial activity, and apoptosis of CECs. Additionally, the metabolic activity of cells was evaluated using FLIM by measuring the fluorescence lifetime of NADH and FAD. Results: When CECs were incubated with MB, there was an increase in mitochondrial activity that was dependent on the concentration of MB. Additionally, mitochondrial activity increased when the CECs were exposed to 660 nm laser irradiation at an energy dose of up to 5 J/cm2. Following PDT, a slight shift towards oxidative phosphorylation was observed. Conclusions: In vitro application of MB accumulation or laser irradiation causes a shift in the redox status of CECs towards increased reducing activity, without causing any cell damage. However, the combined action of PS and laser radiation has the opposite effect on the redox status of cells, resulting in an increase in the oxidized form of FAD.
- ПубликацияТолько метаданныеPHOTO AND SPECTRAL FLUORESCENCE ANALYSIS OF THE SPINAL CORD INJURY AREA IN ANIMAL MODELS ФОТО И СПЕКТРАЛЬНЫИ ФЛУОРЕСЦЕНТНЫИ АНАЛИЗ ОБЛАСТИ ТРАВМЫ СПИННОГО МОЗГА НА ЖИВОТНЫХ МОДЕЛЯХ(2023) Udeneev, A. M.; Kalyagina, N. A.; Skobeltsin, A. S.; Loschenov, V. B.; Калягина, Нина Анатольевна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор БорисовичThe purpose of the work is to follow the dynamics of changes in fluorescent signals in the near-surface layers of tissue of injured areas of the back of laboratory animals, which will allow, by indirect evidence, to evaluate the information content of fluorescence diagnosis for subsequent possible diagnostic monitoring of photodynamic therapy of the spinal cord. The model animals were Wistar rats. Two types of contusions were modeled: pneumo-contusion and contusion by a falling load. Methylene blue and indocyanine green were used as photosensitizers. Fluorescence measurements were carried out by imaging and spectrometric methods. A stroboscopic fluorescence imager with an excitation wavelength of 630 nm was used to acquire fluorescence images. The LESA-01-BIOSPEC spectrometer with a He-Ne laser excitation allowed to obtain spectra. It was shown that both methods make it possible to estimate the fluorescence value of methylene blue and indocyanine green in the tissues under study. Moreover, the photographic method also allows to obtain the spatial distribution of fluorescence. The general trend found in the data is a more intense and uniform fluorescence of the dorsal region of rats with methylene blue and a less intense, but more contrasting distribution of indocyanine green. The presented methods are non-invasive, which makes them attractive for diagnostic use. However, due to the shallow depth of signal reception, the condition of the spine can be determined only indirectly, by the condition of the near-surface layers of tissue that accumulate the photosensitizer.
- ПубликацияТолько метаданныеMethylene blue in anticancer photodynamic therapy: systematic review of preclinical studies(2023) Taldaev, A.; Terekhov, R.; Nikitin, I.; Melnik, E.; Loschenov, V.; Лощенов, Виктор БорисовичBackground: Methylene blue has a long history of clinical application. Thanks to phenothiazine chromophore, it has potential in photodynamic anticancer therapy. In spite of the growing body of literature that has evaluated the action of this dye on different types of cancer, the systematic understanding of this problem is still lacking. Therefore, this systematic review was performed to study the efficacy of methylene blue in photodynamic anticancer therapy. Methods: This systematic review was carried out in accordance with the PRISMA guidelines, and the study protocol was registered in PROSPERO (CRD42022368738). Articles for the systematic review were identified through the PubMed database. SYRCLE s risk of bias tool was used to assess the studies. The results of systematic analysis are presented as narrative synthesis. Results: Ten studies met the inclusion criteria and these full texts were reviewed. In the selected articles, the dosage of dye infusion ranged from 0.04 to 24.12 mg/kg. The effectiveness of photodynamic therapy with methylene blue against different types of cancer was confirmed by a decrease in tumor sizes in seven articles. Conclusion: The results of the systematic review support the suggestions that photodynamic therapy with methylene blue helps against different types of cancer, including colorectal tumor, carcinoma, and melanoma. In cases of nanopharmaceutics use, a considerable increase of anticancer therapy effectiveness was observed. The further research into methylene blue in photodynamic anticancer therapy is needed. Systematic Review Registration: (https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=368738), identifier (CRD42022368738).
- ПубликацияТолько метаданныеPhotodynamic inactivation of bacteria: Why it is not enough to excite a photosensitizer(2023) Meerovich, G. A.; Akhlyustina, E. V.; Romanishkin, I. D.; Loschenov, V. B.; Меерович, Геннадий Александрович; Ахлюстина, Екатерина Витальевна; Лощенов, Виктор Борисович
- ПубликацияТолько метаданныеSPECTROSCOPIC STUDY OF METHYLENE BLUE IN VIVO: EFFECTS ON TISSUE OXYGENATION AND TUMOR METABOLISM СПЕКТРОСКОПИЧЕСКОЕ ИССЛЕДОВАНИЕ МЕТИЛЕНОВОГО СИНЕГО IN VIVO: ВЛИЯНИЕ НА ОКСИГЕНАЦИЮ ТКАНЕИ И ОПУХОЛЕВЫИ МЕТАБОЛИЗМ(2023) Pominova, D. V.; Ryabova, A. V.; Skobeltsin, A. S.; Markova, I. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович
- ПубликацияОткрытый доступOptical spectral diagnostics of the oxygenation level in periodontal tissues and photodynamic therapy using methylene blue in children with cerebral palsy(2023) Morozova, N. S.; Kozlitina, Iu. A.; Makarov, V. I.; Loschenov, V. B.; Макаров, Владимир Игоревич; Лощенов, Виктор БорисовичDental diseases occur in children with cerebral palsy three times higher than in healthy children. Low values of the unstimulated salivation rate (andamp;lt;0.3 ml per minute), pH and buffer capacity, changes in enzyme activity and sialic acid concentration, as well as increased saliva osmolarity and total protein concentration, which indicates impaired hydration, are the factors in the development of a gingiva disease in case of cerebral palsy. This leads to increased bacterial agglutination and the formation of acquired pellicle and biofilm, leading to the formation of dental plaque. There is a tendency toward an increase in the concentration of hemoglobin and a decrease in the degree of hemoglobin oxygenation, as well as an increase in the generation of reactive oxygen and nitrogen species. Photodynamic therapy (PDT) with the use of photosensitizer methylene blue improves blood circulation and the degree of oxygenation in periodontal tissues, as well as eliminates a bacterial biofilm. Analysis of back diffuse reflection spectra makes it possible to conduct non-invasive monitoring determine tissue areas with a low level of hemoglobin oxygenation for precision photodynamic exposure. Aim To improve the effectiveness of phototheranostics methods using, namely PDT with simultaneous optical-spectral control, for the treatment of gingivitis in children with complex dental and somatic status (cerebral palsy). Methods The study involved 15 children (6-18 y.o.) with various forms of cerebral palsy, in particular, spastic diplegia and atonic-astatic form and with gingivitis. The degree of hemoglobin oxygenation was measured in tissues before PDT and on the 12th day. PDT was performed using laser radiation (О» = 660 nm) with a power density of 150 mW/cm 2 with a five-minute application of 0.01% MB. The total light dose was 45 В± 15 J/cm 2 . For statistical evaluation of the results, a paired Student s t-test was used. Results The paper presents the results of phototheranostics using methylene blue in children with cerebral palsy. An increase in the level of hemoglobin oxygenation from 50 to 67% ( p andamp;lt; 0.001) and a decrease in blood volume in the microcirculatory bed of periodontal tissues were shown. Conclusion Photodynamic therapy methods with application of methylene blue make it possible to assess the state of the gingival mucosa tissue diseases objectively in real time, and to provide effective targeted therapy for gingivitis in children with cerebral palsy. There is a prospect that they can become widely used clinical methods.