Персона: Лощенов, Виктор Борисович
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Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
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- ПубликацияОткрытый доступEffect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro(2024) Makarov, V. I.; Skobeltsin, A. S.; Averchuk, A. S.; Loschenov, V. B.; Макаров, Владимир Игоревич; Скобельцин, Алексей Сергеевич; Лощенов, Виктор БорисовичBackground: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. Methods: The study was performed on the primary culture of CECs. The cells underwent photodynamic treatment through 660 nm laser irradiation at a power density of 300 mW/cm2. The MTT, TMRE, and TUNEL assays were used to assess the survival, redox metabolism, mitochondrial activity, and apoptosis of CECs. Additionally, the metabolic activity of cells was evaluated using FLIM by measuring the fluorescence lifetime of NADH and FAD. Results: When CECs were incubated with MB, there was an increase in mitochondrial activity that was dependent on the concentration of MB. Additionally, mitochondrial activity increased when the CECs were exposed to 660 nm laser irradiation at an energy dose of up to 5 J/cm2. Following PDT, a slight shift towards oxidative phosphorylation was observed. Conclusions: In vitro application of MB accumulation or laser irradiation causes a shift in the redox status of CECs towards increased reducing activity, without causing any cell damage. However, the combined action of PS and laser radiation has the opposite effect on the redox status of cells, resulting in an increase in the oxidized form of FAD.
- ПубликацияТолько метаданныеPHOTO AND SPECTRAL FLUORESCENCE ANALYSIS OF THE SPINAL CORD INJURY AREA IN ANIMAL MODELS ФОТО И СПЕКТРАЛЬНЫИ ФЛУОРЕСЦЕНТНЫИ АНАЛИЗ ОБЛАСТИ ТРАВМЫ СПИННОГО МОЗГА НА ЖИВОТНЫХ МОДЕЛЯХ(2023) Udeneev, A. M.; Kalyagina, N. A.; Skobeltsin, A. S.; Loschenov, V. B.; Калягина, Нина Анатольевна; Скобельцин, Алексей Сергеевич; Лощенов, Виктор БорисовичThe purpose of the work is to follow the dynamics of changes in fluorescent signals in the near-surface layers of tissue of injured areas of the back of laboratory animals, which will allow, by indirect evidence, to evaluate the information content of fluorescence diagnosis for subsequent possible diagnostic monitoring of photodynamic therapy of the spinal cord. The model animals were Wistar rats. Two types of contusions were modeled: pneumo-contusion and contusion by a falling load. Methylene blue and indocyanine green were used as photosensitizers. Fluorescence measurements were carried out by imaging and spectrometric methods. A stroboscopic fluorescence imager with an excitation wavelength of 630 nm was used to acquire fluorescence images. The LESA-01-BIOSPEC spectrometer with a He-Ne laser excitation allowed to obtain spectra. It was shown that both methods make it possible to estimate the fluorescence value of methylene blue and indocyanine green in the tissues under study. Moreover, the photographic method also allows to obtain the spatial distribution of fluorescence. The general trend found in the data is a more intense and uniform fluorescence of the dorsal region of rats with methylene blue and a less intense, but more contrasting distribution of indocyanine green. The presented methods are non-invasive, which makes them attractive for diagnostic use. However, due to the shallow depth of signal reception, the condition of the spine can be determined only indirectly, by the condition of the near-surface layers of tissue that accumulate the photosensitizer.
- ПубликацияТолько метаданныеSPECTROSCOPIC STUDY OF METHYLENE BLUE IN VIVO: EFFECTS ON TISSUE OXYGENATION AND TUMOR METABOLISM СПЕКТРОСКОПИЧЕСКОЕ ИССЛЕДОВАНИЕ МЕТИЛЕНОВОГО СИНЕГО IN VIVO: ВЛИЯНИЕ НА ОКСИГЕНАЦИЮ ТКАНЕИ И ОПУХОЛЕВЫИ МЕТАБОЛИЗМ(2023) Pominova, D. V.; Ryabova, A. V.; Skobeltsin, A. S.; Markova, I. V.; Loschenov, V. B.; Поминова, Дарья Вячеславовна; Рябова, Анастасия Владимировна; Лощенов, Виктор Борисович