Персона:
Климентов, Сергей Михайлович

Научные группы

Научная группа

Организационные подразделения

Организационная единица

Инженерно-физический институт биомедицины

Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.

Статус

Руководитель научной группы "Центр нанобиомедицины"

Фамилия

Климентов

Имя

Сергей Михайлович

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Теперь показываю 1 - 10 из 43

Только метаданные
International symposium and international school for young scientists on "physics, engineering and technologies for biomedicine"
(2021) Kabashin, A. V.; Klimentov, S. M.; Timoshenko, V. Yu.; Fronya, A. A.; Кабашин, Андрей Викторович; Климентов, Сергей Михайлович; Тимошенко, Виктор Юрьевич; Фроня, Анастасия Андреевна
Abstract International Symposium and International School for Young Scientists on вЂњPhysics, Engineering and Technologies for BiomedicineвЂќ (PhysBioSymp) is an annually held event in National Research Nuclear University MEPhI (https://eng.mephi.ru/) (Moscow, Russia) since 2016. This symposium is conceived as the main conference of the Institute of Engineering Physics for Biomedicine (PhysBio, https://physbio.mephi.ru/), which is one of new strategic academic units founded in 2016 in the course of transformation of MEPhI from the technical to a global university in order to extend its portfolio toward life sciences, chemical and biomedical engineering. Following the chosen strategy, PhysBio aims at the advancement of its international reputation in biomedical sciences and technologies, as well as the integration of latest research achievements into the educational process in order to contribute to the solution of global problems, such as early diagnostics and efficient therapy of socially significant diseases, including cancer. Conceived as an essentially interdisciplinary institution, PhysBio is unique in combining powerful background of MEPhI in physics, mathematics, engineering, material sciences, nanotechnologies and expertise in new areas in chemistry and biology in order to develop breakthrough technologies for biomedical applications. List of Committees are available in this pdf.
Только метаданные
Cytotoxicity of Laser-Synthesized Nanoparticles of Elemental Bismuth
(2024) Shakhov, P. V.; Tikhonowski, G. V.; Popov, A. A.; Iliasov, A. R.; Lebedev, A. A.; Klimentov, S. M.; Шахов, Павел Владимирович; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Илясов, Артём Романович; Лебедев, Анатолий Алексеевич; Климентов, Сергей Михайлович
Только метаданные
Laser-Ablative Engineering of ZrN-Based Nanoparticles for Photothermal Therapy and SERS-Based Biological Imaging
(2024) Pastukhov, A. I.; Babkova, J. S.; Zelepukin, I. V.; Popov, A. A.; Klimentov, S. M.; Prasad, P. N.; Deyev, S. M.; Бабкова, Юлия Сергеевна; Попов, Антон Александрович; Климентов, Сергей Михайлович; Деев, Сергей Михайлович
Только метаданные
Boron Nanoparticle-Enhanced Proton Therapy: Molecular Mechanisms of Tumor Cell Sensitization
(2024) Popov, A. L.; Kolmanovich, D. D.; Chukavin, N. N.; Zelepukin, I. V.; Tikhonowski, G. V.; Popov, A. A.; Klimentov, S. M.; Deyev, S. M.; Zavestovskaya, I. N.; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Климентов, Сергей Михайлович; Деев, Сергей Михайлович; Завестовская, Ирина Николаевна
Boron-enhanced proton therapy has recently appeared as a promising approach to increase the efficiency of proton therapy on tumor cells, and this modality can further be improved by the use of boron nanoparticles (B NPs) as local sensitizers to achieve enhanced and targeted therapeutic outcomes. However, the mechanisms of tumor cell elimination under boron-enhanced proton therapy still require clarification. Here, we explore possible molecular mechanisms responsible for the enhancement of therapeutic outcomes under boron NP-enhanced proton therapy. Spherical B NPs with a mode size of 25 nm were prepared by methods of pulsed laser ablation in water, followed by their coating by polyethylene glycol to improve their colloidal stability in buffers. Then, we assessed the efficiency of B NPs as sensitizers of cancer cell killing under irradiation with a 160.5 MeV proton beam. Our experiments showed that the combined effect of B NPs and proton irradiation induces an increased level of superoxide anion radical generation, which leads to the depolarization of mitochondria, a drop in their membrane mitochondrial potential, and the development of apoptosis. A comprehensive gene expression analysis (via RT-PCR) confirmed increased overexpression of 52 genes (out of 87 studied) involved in the cell redox status and oxidative stress, compared to 12 genes in the cells irradiated without B NPs. Other possible mechanisms responsible for the B NPs-induced radiosensitizing effect, including one related to the generation of alpha particles, are discussed. The obtained results give a better insight into the processes involved in the boron-induced enhancement of proton therapy and enable one to optimize parameters of proton therapy in order to maximize therapeutic outcomes.
Только метаданные
Nanoparticles based on MIL-101 metal-organic frameworks as efficient carriers of therapeutic188Re radionuclide for nuclear medicine
(2024) Belyaev, I. B.; Zelepukin, I. V.; Petriev, V. M.; Klimentov, S. M.; Zavestovskaya, I. N.; Deyev, S. M.; Климентов, Сергей Михайлович; Завестовская, Ирина Николаевна; Деев, Сергей Михайлович
Только метаданные
Photoluminescent Si-based nanocrystals prepared by pulsed laser ablation in low-pressure helium-nitrogen mixtures for biomedical applications
(2020) Kharin, A. Y.; Fronya, A. A.; Antonenko, S. V.; Karpov, N. V.; Derzhavin, S. I.; Dombrovska, Y. I.; Garmash, A. A.; Kargin, N. I.; Klimentov, S. M.; Timoshenko, V. Y.; Kabashin, A. V.; Фроня, Анастасия Андреевна; Антоненко, Сергей Васильевич; Гармаш, Александр Александрович; Каргин, Николай Иванович; Климентов, Сергей Михайлович; Тимошенко, Виктор Юрьевич; Кабашин, Андрей Викторович
© 2020 SPIE.Nanocrystalline silicon (Si) films were synthesized by nanosecond laser ablation of crystalline Si targets in low-pressure helium (He) and nitrogen (N2) gas mixtures. Photoluminescence (PL) spectra of the prepared samples were found to depend on the He/N2 ratio in the gas mixture. The ablation pure He atmosphere allowed us to prepare Si nanocrystals (NCs) exhibiting a PL band in red-near-IR range, while samples prepared in the presence of N2 exhibited a strong PL band with maximum in the green-yellow region. Such a modification of PL properties can be explained by the presence of amorphous Si oxynitride (a-SiNxOy) on the surface of Si-NCs. Structural studies of the prepared samples by means of the scanning electron microscopy revealed different morphology for Si-NCs produced under different gas mixtures. After treating of the films by ultrasound and dispersing in water, Si-NCs can be used as novel biodegradable markers for bioimaging, while the observed spectral tailoring effect makes possible an adjustment of the PL emission of such markers to a concrete bioimaging task.
Только метаданные
Colloidal samarium oxide nanoparticles prepared by femtosecond laser ablation and fragmentation for nuclear nanomedicine
(2020) Duflot, V. R.; Popova-Kuznetsova, E.; Tikhonowski, G.; Popov, A. A.; Deyev, S. M.; Klimentov, S. M.; Zavestovskaya, I. N.; Prasad, P. N.; Kabashin, A. V.; Попова-Кузнецова, Елена Алефтиновна; Тихоновский, Глеб Валерьевич; Попов, Антон Александрович; Деев, Сергей Михайлович; Климентов, Сергей Михайлович; Завестовская, Ирина Николаевна; Кабашин, Андрей Викторович
© 2020 SPIE.Nanotechnology promises a major improvement of efficacy of nuclear medicine by targeted delivery of radioactive agents to tumors, but this approach still needs novel efficient nanoformulations to maximize diagnostic and therapeutic functions. Here, we present a two-step method of laser ablation and fragmentation in water to produce non-radioactive 152Sm-enriched samarium oxide nanoparticles (Sm NPs), which can be converted to radioactive form of 153Sm beta-emitters by neutron capture reaction. We found that laser ablation in deionized water leads to the formation of NPs having diverse morphology and broad size dispersion. To improve size characteristics of formed NPs, we applied additional femtosecond laser fragmentation step, which made possible a good control of mean NPs size under a drastic narrowing of size dispersion, and the spherical shape of formed NPs. Obtained colloidal solutions of Sm NPs were stable for several weeks after the synthesis. The formed NPs present a very promising object for nuclear nanomedicine.
Только метаданные
Interaction of SiFe Nanoparticles with Epithelial and Lymphoid Cells
(2020) Sharonova, N. V.; Svirshchevskaya, E. V.; Popov, A. A.; Karpov, N. V.; Tikhonovsky, G. V.; Zakharkiv, A. Y.; Timoshenko, V. Y.; Klimentov, S. M.; Oleinikov, V. A.; Попов, Антон Александрович; Тихоновский, Глеб Валерьевич; Захаркив, Анастасия Юрьевна; Тимошенко, Виктор Юрьевич; Климентов, Сергей Михайлович; Олейников, Владимир Александрович
Silicon and silicon-based nanoparticles (SiNP) attract scientific attention due to the biocompatibility and assimilation of silicon by body tissues. Iron-doped SiNP (SiFeNP) allow the use of ferromagnetic properties of iron for NP detection and the possibility of therapeutic application of SiFeNP. The purpose of this work was to analyze the interaction of SiFeNP with epithelial cells (EC) COLO357 and SW620 and human peripheral blood lymphocytes (PBL). SiFeNP were obtained by laser ablation and divided into the NP1 and NP2 fractions of 100 and 150 nm size, respectively. Cytotoxicity, apoptosis induction, reactive oxygen species (ROS) production, and lysosome metabolism were analyzed using in vitro methods. EC were found to efficiently incytosed both types of NPs, which resulted in the increase in the granularity of cells. NP did not cause apoptosis or EC necrosis, but accumulated in lysosomes, which led to a decrease in the membrane potential of lysosomes. In turn, a decrease in the level of EC metabolism led to a gradual (24 h) increase in ROS production by 10-15%. NP1 caused more ROS than NP2, and accumulated more in the EC, which may be the result of a difference in the particle size. SiFeNP did not interact with PBL. Thus, the total cytotoxicity of SiFeNP did not exceed 20%, which is associated with a decrease in lysosome metabolism and insignificant ROS production.
Только метаданные
Nonlinear photoacoustic response of suspensions of laser-synthesized plasmonic titanium nitride nanoparticles
(2020) Maldonado, M. E.; Das, A.; Gomes, A. S. L.; Popov, A. A.; Klimentov, S. M.; Kabashin, A. V.; Попов, Антон Александрович; Климентов, Сергей Михайлович; Кабашин, Андрей Викторович
© 2020 Optical Society of AmericaA nonlinear photoacoustic (PA) response from solutions of 40 nm plasmonic titanium nitride nanoparticles (NPs) synthesized by laser ablation in a liquid environment (acetone) is reported. Using a photoacoustic Z-scan with 5 ns pumping pulses, values of effective nonlinear absorption (NLA) coefficients βPA,eff were measured and found to be 3.27±0.17 × 10−8, 6.41±0.32 × 10−8, and 3.22±0.16 × 10−8 for 600, 700, and 800 nm pumping wavelengths, respectively. To take into account the influence of nonlinear scattering, absorption-dependent PA measurements were carried out together with the optical Z-scan, and the obtained data were compared. The origin of the effective absorptive nonlinearity is discussed based on combined NLA in NPs, nonlinear scattering, and bubble generation triggered by NP-mediated light absorption. Potential applications include biomedical diagnostics and therapy.
Только метаданные
Evolution of the properties of nanomaterials obtained by pulsed laser ablation
(2020) Faizullayeva, S.; Klimentov, S. M.; Popov, A. A.; Файзуллаева, Сабина Юсуповна; Климентов, Сергей Михайлович; Попов, Антон Александрович
© NANOCON 2019.All right reserved.The synthesis and application of metal and ceramic nanoparticle are significant subject in science and engineering. The metal nanoparticles such as gold, silver, and copper nanoparticles have more application in material science, nanomedicine, electronic, photonic, and art. One of the green method for preparation of metal nanoparticles is laser ablation technique that offers a unique tool for nanofabrication of nanoparticles. In this work, gold colloids were prepared by pulsed laser ablation of high purity gold plate immersed in deionized water and 10µM NaCl solution. After ablation process we study the change in the size distribution of nanoparticles after the ablation process for a month.