Publication:
Barnase*Barstar-guided two-step targeting approach for drug delivery to tumor cells in vivo

Дата
2021
Авторы
Shramova, E. I.
Shilova, M. V.
Ryabova, A. V.
Dzhalilova, D. S.
Deyev, S.
Journal Title
Journal ISSN
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Научные группы
Организационные подразделения
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Организационная единица
Инженерно-физический институт биомедицины
Цель ИФИБ и стратегия развития – это подготовка высококвалифицированных кадров на базе передовых исследований и разработок новых перспективных методов и материалов в области инженерно-физической биомедицины. Занятие лидерских позиций в биомедицинских технологиях XXI века и внедрение их в образовательный процесс, что отвечает решению практикоориентированной задачи мирового уровня – диагностике и терапии на клеточном уровне социально-значимых заболеваний человека.
Выпуск журнала
Аннотация
© 2021 Elsevier B.V.For precise ligation of a targeting and cytotoxic moiety, the use of Barnase-Barstar pair as a molecular glue is proposed for the first time. Targeting was mediated through the use of a scaffold protein DARPin_9–29 specific for the human epidermal receptor 2 (HER2) antigen that is highly expressed on some types of cancer and Barnase*Barstar native bacterial proteins interacted with each other with Kd 10−14 M. The approach proposed consists of prelabeling a target tumor with hybrid protein DARPin-Barnase prior to administration of cytotoxic component-loaded liposomes that have Barstar covalently attached to their surface. Based on in vivo bioimaging we have proven that DARPin-based Barnase*Barstar-mediated pretargeting possesses precise tumor-targeting capability as well as antitumor activity leading to apparent tumor-growth inhibition of primary tumors and distant metastases in experimental animals. The results obtained indicate that the new system combining DARPin and Barnase*Barstar can be useful both for the drug development and for monitoring the response to treatment in vivo in preclinical studies.
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Цитирование
Barnase*Barstar-guided two-step targeting approach for drug delivery to tumor cells in vivo / Shramova, E.I. [et al.] // Journal of Controlled Release. - 2021. - 340. - P. 200-208. - 10.1016/j.jconrel.2021.11.001
URI
https://www.doi.org/10.1016/j.jconrel.2021.11.001
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85118600747&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000719074200007
 https://openrepository.mephi.ru/handle/123456789/24873
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