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A robust method for adjustment of laser speckle contrast imaging during transcranial mouse brain visualization

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dc.contributor.authorKalchenko, V.
dc.contributor.authorSdobnov, A.
dc.contributor.authorKuznetsov, Y.
dc.contributor.authorMolodij, G.
dc.contributor.authorMeglinski, I.
dc.date.accessioned2024-11-21T11:36:20Z
dc.date.available2024-11-21T11:36:20Z
dc.date.issued2019
dc.description.abstract© 2019 by the authors.Laser speckle imaging (LSI) is a well-known and useful approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues (such as brain vasculature, skin capillaries etc.). Despite an extensive use of LSI for brain imaging, the LSI technique has several critical limitations. One of them is associated with inability to resolve a functionality of vessels. This limitation also leads to the systematic error in the quantitative interpretation of values of speckle contrast obtained for different vessel types, such as sagittal sinus, arteries, and veins. Here, utilizing a combined use of LSI and fluorescent intravital microscopy (FIM), we present a simple and robust method to overcome the limitations mentioned above for the LSI approach. The proposed technique provides more relevant, abundant, and valuable information regarding perfusion rate ration between different types of vessels that makes this method highly useful for in vivo brain surgical operations.
dc.identifier.citationA robust method for adjustment of laser speckle contrast imaging during transcranial mouse brain visualization / Kalchenko, V. [et al.] // Photonics. - 2019. - 6. - № 3. - 10.3390/photonics6030080
dc.identifier.doi10.3390/photonics6030080
dc.identifier.urihttps://www.doi.org/10.3390/photonics6030080
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85071565090&origin=resultslist
dc.identifier.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000488009300024
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/18638
dc.relation.ispartofPhotonics
dc.titleA robust method for adjustment of laser speckle contrast imaging during transcranial mouse brain visualization
dc.typeLetter
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.volume6
relation.isOrgUnitOfPublicationc8407a6f-7272-450d-8d99-032352c76b55
relation.isOrgUnitOfPublication.latestForDiscoveryc8407a6f-7272-450d-8d99-032352c76b55
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