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The use of methylene blue to control the tumor oxygenation level

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dc.contributor.authorPominova, D.
dc.contributor.authorRyabova, A.
dc.contributor.authorSkobeltsin, A.
dc.contributor.authorMarkova, I.
dc.contributor.authorПоминова, Дарья Вячеславовна
dc.contributor.authorРябова, Анастасия Владимировна
dc.contributor.authorСкобельцин, Алексей Сергеевич
dc.date.accessioned2024-12-04T10:29:56Z
dc.date.available2024-12-04T10:29:56Z
dc.date.issued2024
dc.description.abstractHypoxia is a characteristic feature of many tumors. It promotes tumor proliferation, metastasis, and invasion and can reduce the effectiveness of many types of cancer treatment. The aim of this study was to investigate the pharmacokinetics of methylene blue (MB) and its impact on the tumor oxygenation level at mouse Lewis lung carcinoma (LLC) model using spectroscopic methods. The pharmacokinetics of MB were studied qualitatively and quantitatively using video fluorescence imaging and fluorescence spectroscopy. The degree of hemoglobin oxygenation in vivo was examined by calculating hemoglobin optical absorption from the measured diffuse reflectance spectra. The distribution of MB fluorescence and the lifetime of NADH were analyzed using laser scanning microscopy and fluorescence lifetime imaging microscopy (FLIM) to assess cellular metabolism. After intravenous administration of MB at 10ў??20 mg/kg, it quickly transitioned in the tumor to a colorless leucomethylene blue, with maximum accumulation in the tumor occurring after 5ў??10 minutes. A concentration of 10 mg/kg resulted in a relative increase of the tumor oxygenation level for small tumors (volume 50ў??75 mm3) and normal tissue 120 minutes after the introduction of MB. A shift in tumor metabolism towards oxidative phosphorylation (according to the lifetime of the NADH coenzyme) was measured using FLIM method after intravenous administration of 10 mg/kg of MB. Intravenous administration of MB at 20 mg/kg results in a long-term decrease in oxygenation, which persisted for at least 120 minutes after the administration and did not return to its initial level. Administration of MB at 10 mg/kg shown to increase tumor oxygenation level, potentially leading to more effective antitumor therapy. However, at higher doses (20 mg/kg), MB may cause long-term decrease in oxygenation.
dc.identifier.citationThe use of methylene blue to control the tumor oxygenation level / Pominova, D. [et al.] // Photodiagnosis and Photodynamic Therapy. - 2024. - 46. - 10.1016/j.pdpdt.2024.104047
dc.identifier.doi10.1016/j.pdpdt.2024.104047
dc.identifier.urihttps://www.doi.org/10.1016/j.pdpdt.2024.104047
dc.identifier.urihttps://www.scopus.com/record/display.uri?eid=2-s2.0-85189526004&origin=resultslist
dc.identifier.urihttps://openrepository.mephi.ru/handle/123456789/25921
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.subjectMethylene blue
dc.subjectTumor Imaging
dc.subjectTumor Hypoxia
dc.subjectTumor Microenvironment
dc.subjectCancer Cell Metabolism
dc.subjectTumor Targeting
dc.titleThe use of methylene blue to control the tumor oxygenation level
dc.typeArticle
dspace.entity.typePublication
oaire.citation.volume46
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