Publication:
Myelin basic protein dynamics from out-of-equilibrium functional state to degraded state in myelin

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W3010432675.pdf(2.35 MB)
Дата
2020
Авторы
Di, Gioacchino, M.
Burghammer, M.
Ciasca, G.
Bruni, F.
Bianconi, A.
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Научные группы
Организационные подразделения
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Организационная единица
Институт лазерных и плазменных технологий
Стратегическая цель Института ЛаПлаз – стать ведущей научной школой и ядром развития инноваций по лазерным, плазменным, радиационным и ускорительным технологиям, с уникальными образовательными программами, востребованными на российском и мировом рынке образовательных услуг.
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Аннотация
© 2020 Elsevier B.V.Living matter is a quasi-stationary out-of-equilibrium system; in this physical condition, structural fluctuations at nano- and meso-scales are needed to understand the physics behind its biological functionality. Myelin has a simple ultrastructure whose fluctuations show correlated disorder in its functional out-of-equilibrium state. However, there is no information on the relationship between this correlated disorder and the dynamics of the intrinsically disordered Myelin Basic Protein (MBP) which is expected to influence the membrane structure and overall functionality. In this work, we have investigated the role of this protein structural dynamics in the myelin ultrastructure fluctuations in various conditions, by using synchrotron Scanning micro X Ray Diffraction and Small Angle X ray Scattering. We have induced the crossover from out-of-equilibrium functional state to in-equilibrium degeneration changing the pH to values far from physiological condition. The observed compression of the cytosolic layer thickness probes that the intrinsic large MBP fluctuations preserve the cytosol structure also in the degraded state. Thus, the transition of myelin ultrastructure from correlated to uncorrelated disordered state, is principally affected by the deformation of the membrane and extracellular domain.
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Цитирование
Myelin basic protein dynamics from out-of-equilibrium functional state to degraded state in myelin / Di, Gioacchino, M. [et al.] // Biochimica et Biophysica Acta - Biomembranes. - 2020. - 1862. - № 6. - 10.1016/j.bbamem.2020.183256
URI
https://www.doi.org/10.1016/j.bbamem.2020.183256
 https://www.scopus.com/record/display.uri?eid=2-s2.0-85081251675&origin=resultslist
 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS_CPL&DestLinkType=FullRecord&UT=WOS:000528195500021
 https://openrepository.mephi.ru/handle/123456789/20376
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